Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética
Autor(a) principal: | |
---|---|
Data de Publicação: | 2014 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/12638 |
Resumo: | Nutritional changes during critical developmental periods are associated with chronic diseases in adulthood, a phenomenon known as developmental plasticity. Osteogenesis and adipogenesis have common mechanisms. In 3 different models of developmental plasticity, we observed programming for obesity. Thus, the aim of this study was to investigate the impact of these 3 models, mechanical early weaning (MEW), pharmacological early weaning (PEW), and early overnutrition (EO) upon offspring s bone tissue during development. Thus, the present study was divided into two experiments. In experiment 1, lactating rats were separated into 3 groups: control - pups had free access to milk; MEW - dams were involved with a bandage interrupting lactation in the last 3 days; PEW - dams were pharmacologically treated to block prolactin (0.5 mg bromocryptine/twice a day) 3 days before standard weaning. In experiment 2, litter size was adjusted to 3 male rats per litter (EO). Litter containing 10 pups per mother was control. Bone tissue was evaluated by dual-energy X-ray absorptiometry, computed tomography, microcomputed tomography, biomechanical tests and serum analyses. Data significant had P<0.05. In experiment 1, at weaning, MEW and PEW pups presented lower body weight, total body fat, total bone mineral density (BMD), total bone mineral content (BMC), bone area, serum osteocalcin and higher C-terminal cross-linked telopeptide of type I collagen (CTX-I). However, serum ionized calcium was lower only in MEW pups, 25-hydroxyvitamin D was higher and PTH was lower only in PEW pups. In adulthood, MEW and PEW groups presented higher body weight, visceral fat mass, 25-hydroxivitamin D, hyperleptinemia and lower CTX-I. Both groups presented higher total BMD, total BMC, spine BMD and bone area in 150 and 180 days. In individual bone evaluations, MEW and PEW offspring also showed higher femur BMD and fourth lumbar vertebra (L4) BMD, femoral head radiodensity and L4 vertebral body radiodensity, better bone trabecular microarchitecture and bone strength. In experiment 2, EO offspring showed higher body weight, fat mass, lean mass, BMC and bone area from weaning to adulthood. At 180 days, EO offspring also presented higher total BMD, femur BMD, L4 BMD, femoral head and vertebral body radiodensity, better bone trabecular microarchitecture and bone strength, higher osteocalcin and lower CTX-I. The three models lead to obesity and it seems that independent of the imprinting factors, there is a protective effect on bone tissue, which probably occurs as a result of different hormonal changes, that put together with the mechanical effect caused by excess of body fat mass, help us to explain the better bone health observed in these models of obesity. |
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Silva, Patrícia Cristina Lisbôa dahttp://lattes.cnpq.br/6704904748735082Moura, Egberto Gaspar dehttp://lattes.cnpq.br/9398848717949756Nunes, Maria Terezahttp://lattes.cnpq.br/4808814043079266Rocco, Patricia Rieken Macedohttp://lattes.cnpq.br/1800482670266921Farias, Maria Lucia Fleiuss dehttp://lattes.cnpq.br/9638149960657616Carvalho, Jorge José dehttp://lattes.cnpq.br/2608779267915272http://lattes.cnpq.br/3173462238559662Maia, Lígia de Albuquerque2021-01-06T20:53:39Z2015-02-052014-02-21MAIA, Lígia de Albuquerque. Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética. 2014. 101 f. Tese (Doutorado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2014.http://www.bdtd.uerj.br/handle/1/12638Nutritional changes during critical developmental periods are associated with chronic diseases in adulthood, a phenomenon known as developmental plasticity. Osteogenesis and adipogenesis have common mechanisms. In 3 different models of developmental plasticity, we observed programming for obesity. Thus, the aim of this study was to investigate the impact of these 3 models, mechanical early weaning (MEW), pharmacological early weaning (PEW), and early overnutrition (EO) upon offspring s bone tissue during development. Thus, the present study was divided into two experiments. In experiment 1, lactating rats were separated into 3 groups: control - pups had free access to milk; MEW - dams were involved with a bandage interrupting lactation in the last 3 days; PEW - dams were pharmacologically treated to block prolactin (0.5 mg bromocryptine/twice a day) 3 days before standard weaning. In experiment 2, litter size was adjusted to 3 male rats per litter (EO). Litter containing 10 pups per mother was control. Bone tissue was evaluated by dual-energy X-ray absorptiometry, computed tomography, microcomputed tomography, biomechanical tests and serum analyses. Data significant had P<0.05. In experiment 1, at weaning, MEW and PEW pups presented lower body weight, total body fat, total bone mineral density (BMD), total bone mineral content (BMC), bone area, serum osteocalcin and higher C-terminal cross-linked telopeptide of type I collagen (CTX-I). However, serum ionized calcium was lower only in MEW pups, 25-hydroxyvitamin D was higher and PTH was lower only in PEW pups. In adulthood, MEW and PEW groups presented higher body weight, visceral fat mass, 25-hydroxivitamin D, hyperleptinemia and lower CTX-I. Both groups presented higher total BMD, total BMC, spine BMD and bone area in 150 and 180 days. In individual bone evaluations, MEW and PEW offspring also showed higher femur BMD and fourth lumbar vertebra (L4) BMD, femoral head radiodensity and L4 vertebral body radiodensity, better bone trabecular microarchitecture and bone strength. In experiment 2, EO offspring showed higher body weight, fat mass, lean mass, BMC and bone area from weaning to adulthood. At 180 days, EO offspring also presented higher total BMD, femur BMD, L4 BMD, femoral head and vertebral body radiodensity, better bone trabecular microarchitecture and bone strength, higher osteocalcin and lower CTX-I. The three models lead to obesity and it seems that independent of the imprinting factors, there is a protective effect on bone tissue, which probably occurs as a result of different hormonal changes, that put together with the mechanical effect caused by excess of body fat mass, help us to explain the better bone health observed in these models of obesity.O aumento da prevalência da obesidade e osteoporose, bem como a identificação de mecanismos comuns que ligam a osteogênese e a adipogênese, sugerem que a obesidade e osteoporose podem ser distúrbios relacionados, e além disso, ambos podem ter suas origens no início da vida. Em 3 modelos diferentes de plasticidade ontogenética foi observado obesidade na vida adulta. Sendo assim, o objetivo deste trabalho foi investigar o impacto desses 3 modelos, o desmame precoce mecânico (DPM) e o farmacológico (DPF), e a supernutrição neonatal (SN) no tecido ósseo da prole durante o desenvolvimento. Para tanto, 2 experimentos foram realizados. No experimento 1, ratas lactantes foram divididas em 3 grupos: controle - os filhotes tiveram livre acesso ao leite durante toda a lactação; DPM - as mães foram envolvidas com uma atadura nos últimos 3 dias de lactação; DPF - as mães foram tratadas com bromocriptina (0,5 mg/duas vezes/dia) 3 dias antes do desmame padrão. No experimento 2, o tamanho da ninhada foi reduzido para 3 filhotes machos no 3o dia de lactação até o desmame (SN); o grupo controle permaneceu com 10 filhotes durante toda a lactação. Realizou-se absorciometria de raios-x de dupla energia, tomografia computadorizada, microtomografia computadorizada, teste biomecânico e análises séricas. Os dados foram considerados significativos quando P<0,05. No experimento 1, ao desmame, os filhotes DPM e DPF apresentaram menor massa corporal, massa gorda, densidade mineral óssea total (DMO), conteúdo mineral ósseo total (CMO), área óssea e osteocalcina sérica, e maior telopeptídeo carboxi-terminal do colágeno tipo I (CTX-I). O cálcio ionizado sérico foi menor apenas na prole DPM, a 25-hidroxivitamina D (25(OH)D) foi maior e o PTH menor apenas na prole DPF. Aos 180 dias, as proles DPM e DPF apresentaram maior massa corporal, maior massa de gordura visceral, hiperleptinemia, maior 25(OH)D e menor CTX-I. Ambos os grupos apresentaram aumento da DMO total, do CMO, da DMO da coluna vertebral e da área óssea aos 150 e 180 dias de idade. Nas avaliações ósseas individuais, as proles DPM e DPF também apresentaram aumento da DMO do fêmur e da vértebra lombar, da radiodensidade da cabeça femoral e do corpo vertebral; melhora da microarquitetura trabecular óssea e da resistência óssea. No experimento 2, observamos aumento da massa corporal, da massa gorda e da massa magra, do CMO e da área óssea no grupo SN desde o desmame até a idade adulta. Aos 180 dias, a prole SN também apresentou aumento da DMO total, da DMO do fêmur e da vértebra lombar, da radiodensidade da cabeça femoral e do corpo vertebral; melhora da microarquitetura trabecular óssea e da resistência óssea, maior osteocalcina e menor CTX-I. Demonstramos que, apesar de fatores de imprinting opostos, ambos os modelos causam melhora da massa, do metabolismo, da qualidade e da resistência óssea. Porém, parece que este efeito protetor sobre o tecido ósseo não é um resultado direto da programação deste tecido, mas sim consequência das alterações fisiopatológicas da obesidade programada pelos três modelos.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:53:39Z No. of bitstreams: 1 Ligia de Albuquerque Maia Tese completa.pdf: 2606882 bytes, checksum: b0915e433de0988b8c0e47db9d719491 (MD5)Made available in DSpace on 2021-01-06T20:53:39Z (GMT). No. of bitstreams: 1 Ligia de Albuquerque Maia Tese completa.pdf: 2606882 bytes, checksum: b0915e433de0988b8c0e47db9d719491 (MD5) Previous issue date: 2014-02-21Fundação Carlos Chagas Filho de Amparo a Pesquisa do Estado do Rio de Janeiroapplication/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Fisiopatologia Clínica e ExperimentalUERJBRCentro Biomédico::Faculdade de Ciências MédicasBone metabolismOntogenetic plasticityEarly weaningNeonatal overnutritionLeptinMetabolismo ósseoPlasticidade ontogenéticaDesmame precoceSupernutrição neonatalLeptinaOssos Aspectos nutricionaisLactação ControleHipernutriçãoOsteogênese FisiologiaCNPQ::CIENCIAS DA SAUDE::NUTRICAOAvaliação do metabolismo ósseo em modelos de plasticidade ontogenéticaEvaluation of bone metabolism in models of developmental plasticityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALLigia de Albuquerque Maia Tese completa.pdfapplication/pdf2606882http://www.bdtd.uerj.br/bitstream/1/12638/1/Ligia+de+Albuquerque+Maia+Tese+completa.pdfb0915e433de0988b8c0e47db9d719491MD511/126382024-02-26 16:36:29.075oai:www.bdtd.uerj.br:1/12638Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T19:36:29Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
dc.title.alternative.eng.fl_str_mv |
Evaluation of bone metabolism in models of developmental plasticity |
title |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
spellingShingle |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética Maia, Lígia de Albuquerque Bone metabolism Ontogenetic plasticity Early weaning Neonatal overnutrition Leptin Metabolismo ósseo Plasticidade ontogenética Desmame precoce Supernutrição neonatal Leptina Ossos Aspectos nutricionais Lactação Controle Hipernutrição Osteogênese Fisiologia CNPQ::CIENCIAS DA SAUDE::NUTRICAO |
title_short |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
title_full |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
title_fullStr |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
title_full_unstemmed |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
title_sort |
Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética |
author |
Maia, Lígia de Albuquerque |
author_facet |
Maia, Lígia de Albuquerque |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Silva, Patrícia Cristina Lisbôa da |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6704904748735082 |
dc.contributor.advisor-co1.fl_str_mv |
Moura, Egberto Gaspar de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9398848717949756 |
dc.contributor.referee1.fl_str_mv |
Nunes, Maria Tereza |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/4808814043079266 |
dc.contributor.referee2.fl_str_mv |
Rocco, Patricia Rieken Macedo |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1800482670266921 |
dc.contributor.referee3.fl_str_mv |
Farias, Maria Lucia Fleiuss de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9638149960657616 |
dc.contributor.referee4.fl_str_mv |
Carvalho, Jorge José de |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/2608779267915272 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3173462238559662 |
dc.contributor.author.fl_str_mv |
Maia, Lígia de Albuquerque |
contributor_str_mv |
Silva, Patrícia Cristina Lisbôa da Moura, Egberto Gaspar de Nunes, Maria Tereza Rocco, Patricia Rieken Macedo Farias, Maria Lucia Fleiuss de Carvalho, Jorge José de |
dc.subject.eng.fl_str_mv |
Bone metabolism Ontogenetic plasticity Early weaning Neonatal overnutrition Leptin |
topic |
Bone metabolism Ontogenetic plasticity Early weaning Neonatal overnutrition Leptin Metabolismo ósseo Plasticidade ontogenética Desmame precoce Supernutrição neonatal Leptina Ossos Aspectos nutricionais Lactação Controle Hipernutrição Osteogênese Fisiologia CNPQ::CIENCIAS DA SAUDE::NUTRICAO |
dc.subject.por.fl_str_mv |
Metabolismo ósseo Plasticidade ontogenética Desmame precoce Supernutrição neonatal Leptina Ossos Aspectos nutricionais Lactação Controle Hipernutrição Osteogênese Fisiologia |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::NUTRICAO |
description |
Nutritional changes during critical developmental periods are associated with chronic diseases in adulthood, a phenomenon known as developmental plasticity. Osteogenesis and adipogenesis have common mechanisms. In 3 different models of developmental plasticity, we observed programming for obesity. Thus, the aim of this study was to investigate the impact of these 3 models, mechanical early weaning (MEW), pharmacological early weaning (PEW), and early overnutrition (EO) upon offspring s bone tissue during development. Thus, the present study was divided into two experiments. In experiment 1, lactating rats were separated into 3 groups: control - pups had free access to milk; MEW - dams were involved with a bandage interrupting lactation in the last 3 days; PEW - dams were pharmacologically treated to block prolactin (0.5 mg bromocryptine/twice a day) 3 days before standard weaning. In experiment 2, litter size was adjusted to 3 male rats per litter (EO). Litter containing 10 pups per mother was control. Bone tissue was evaluated by dual-energy X-ray absorptiometry, computed tomography, microcomputed tomography, biomechanical tests and serum analyses. Data significant had P<0.05. In experiment 1, at weaning, MEW and PEW pups presented lower body weight, total body fat, total bone mineral density (BMD), total bone mineral content (BMC), bone area, serum osteocalcin and higher C-terminal cross-linked telopeptide of type I collagen (CTX-I). However, serum ionized calcium was lower only in MEW pups, 25-hydroxyvitamin D was higher and PTH was lower only in PEW pups. In adulthood, MEW and PEW groups presented higher body weight, visceral fat mass, 25-hydroxivitamin D, hyperleptinemia and lower CTX-I. Both groups presented higher total BMD, total BMC, spine BMD and bone area in 150 and 180 days. In individual bone evaluations, MEW and PEW offspring also showed higher femur BMD and fourth lumbar vertebra (L4) BMD, femoral head radiodensity and L4 vertebral body radiodensity, better bone trabecular microarchitecture and bone strength. In experiment 2, EO offspring showed higher body weight, fat mass, lean mass, BMC and bone area from weaning to adulthood. At 180 days, EO offspring also presented higher total BMD, femur BMD, L4 BMD, femoral head and vertebral body radiodensity, better bone trabecular microarchitecture and bone strength, higher osteocalcin and lower CTX-I. The three models lead to obesity and it seems that independent of the imprinting factors, there is a protective effect on bone tissue, which probably occurs as a result of different hormonal changes, that put together with the mechanical effect caused by excess of body fat mass, help us to explain the better bone health observed in these models of obesity. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014-02-21 |
dc.date.available.fl_str_mv |
2015-02-05 |
dc.date.accessioned.fl_str_mv |
2021-01-06T20:53:39Z |
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info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
MAIA, Lígia de Albuquerque. Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética. 2014. 101 f. Tese (Doutorado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2014. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/12638 |
identifier_str_mv |
MAIA, Lígia de Albuquerque. Avaliação do metabolismo ósseo em modelos de plasticidade ontogenética. 2014. 101 f. Tese (Doutorado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2014. |
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http://www.bdtd.uerj.br/handle/1/12638 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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Universidade do Estado do Rio de Janeiro |
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Programa de Pós-Graduação em Fisiopatologia Clínica e Experimental |
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UERJ |
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BR |
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Centro Biomédico::Faculdade de Ciências Médicas |
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Universidade do Estado do Rio de Janeiro |
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