Hidatidose policística no norte do Brasil: abordagem parasitológica e histopatológica de pacas e casos humanos

• Autor(a) principal: Cunha, Fernanda Barbosa de Almeida da
• Data de Publicação: 2012
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14354
• topic: Polycystic hydatidosis; Echinococcus vogeli; Morphology; Morphometry; Histopathology; Agouti paca; Human cases; Equinococose; Fígado; Echinococcus; Equinococose Hepática; Parasitologia; Zoonoses; Animais Selvagens; Roedores; Hidatidose policística; Echinococcus vogeli; Morfologia; Morfometria; Histopatologia; Agouti paca; Casos humanos; CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA::HELMINTOLOGIA DE PARASITOS

Polycystic hydatidosis is a zoonotic disease caused by the cestode Echinococcus vogeli, largely distributed in northern Brazil. The definitive hosts are Speothos venaticus (bush dogs) and Canis familiaris (domestic dogs), while Agouti paca (paca) is the intermediate host. Both the pacas and man (accidental host) develop the larval form (metacestodes), mainly on the surface and inside the liver. This thesis aims to study histopathological and parasitological characteristics of metacestodes of E. vogeli, from pacas and humans of the north region of Brazil, seen the insufficient knowledge or lack of it. The mesentery and livers were recovered from eight patients with polycystic hydatidosis during surgery in the Acre State Hospital Foundation. Pacas were captured in the municipality of Bujari, Antimary State Forest, Acre. During the pacas necropsy, macroscopic lesions were observed (whitish or yellowish masses, similar to blisters on the surface of the liver). Identification analyses were applied to light microscopy, interferential contrast microscopy of Normaski (DIC) and confocal laser scanning microscopy. Morphometric analysis was performed by Image Pro Plus Media Cybernetics program. The pacas and human organs were subjected to histopathological analysis. The small and large rostellar hooks presented morphological polymorphism while protoscoleces organisation accompanied the pattern described in Echinococcus sp. All pacas presented liver cysts, however in only two found proven by the presence of hydatid fluid, hooks and protoscoleces. Histopathological analysis of pacas hepatic tissues confirmed polycystic hydatidosis and evidenced by the presence of eggs clusters the Calodium hepaticum coinfection. The morphological characteristics of human cases rostellar hooks did not differ from described to the pacas; however, the hooks were greater than the mesenteric ones. Already in relation to protoscoleces, the mesenteric ones were larger than the liver. Mesenteric and liver cysts presented protoscoleces at different stages of development, with rostellar pad formed by large and small hooks, and two pairs of suckers, in addition to the calcareous corpuscles. Liver cysts showed three characteristics layers (adventitia, laminated and germinative), while mesenteric cysts did not present the adventitia, being the laminated layer the most evidenced in this organ. In the mesentery, mononuclear cells were the principal constituents of leukocyte infiltrated, whose intensity was related to the proliferative capacity of germinative layer. In addition to typical liver cysts of the chronic phase, depending on the inflammatory response, have been observed cysts in acute and subacute phase. It was found a case of co-infection with virus (HIV, HCV and HCB) and another with gallbladder involvement. In short, it is confirmed polycystic hydatidosis in pacas from Acre and is presented new cases of human infection in Acre and Amazonas. For the first time, it was demonstrated polymorphism, different development pattern of cysts depending on the organ, co-infection and gallbladder involvement