Análise fenotípica e genotípica de cepas de Escherichia coli Uropatogênicas (UPEC) isoladas de pacientes da nefrologia do Hospital Universitário Pedro Ernesto (HUPE)

• Autor(a) principal: Souza, Lucieny de Faria
• Data de Publicação: 2018
• Outros Autores: [e-mail]
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/21038
• topic: Virulence factors; Resistance factors; Fosfomycin; Adhesion; Invasion; Phylogeny; Escherichia coli Uropatogênica – Patogenicidade; Fatores de virulência – Genética; Resistência microbiana a medicamentos – Genética; Filogenia; Doenças urológicas – Microbiologia; UPEC; Fatores de resistência; Fosfomicina; Aderência; Invasão; CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA::MICROBIOLOGIA MEDICA

Invasion of bacteria capable of causing cystitis and / or pyelonephritis characterizes UTI. Escherichia coli is the main bacterial species associated with this infection, due to its importance in causing UTI, and some of this species are called UPEC (Uropathogenic Escherichia coli). In the present study, 11 UPEC strains isolated from urine of patients from the HUPE nephrology clinic with symptomatic or asymptomatic infections were studied. The presence of 5 virulence factors was investigated through simple and multiplex PCR techniques to verify the genes encoding adhesins P (pap), fímbria S (sfa), sideroforo (aerobactinin) and alpha-hemolysins (hly); islands of pathogenicity (virulence) through the PAI marker. The antimicrobial resistance profiles and presence of the main resistance genes such as blaNDM-1, blaVIM-1, blaIMP-1, blaKPC (genes encoding the production of metalobetalactamase enzymes) were evaluated; blaCTX (encoding ESBL) and oxa-48 gene (which encodes the production of OXA-carbapenemase). To investigate the pathogenesis of these infections, we used the adhesion and invasion tests in VERO cells where we had 100% adherence and invasion, as well as the use of scanning electron microscopy (SEM), as a methodological alternative for the registration of adhesion and invasion of E in VERO cells. The strains of UPECs tested did not present virulence factors, or had at least one of these factors. We used to characterize the clonal relationship of the strains studied in the study of pulsed field electrophoresis (PFGE) and the phylogenetic relationship by determining the groups A, B1, B2 and D of these microorganisms, which presented as a prevalence of groups D followed by B2. The joint analysis of these data contributed to determine, together with the formation of biofilm and the resistance, that there is no relationship between each other, all these analyzed parameters