Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL

Detalhes bibliográficos
Autor(a) principal: Souza, Aryanna Kelly Pinheiro
Data de Publicação: 2009
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da Universidade Federal de Alagoas (UFAL)
Texto Completo: http://repositorio.ufal.br/handle/riufal/920
Resumo: The fungi are being opportunistic, with wide distribution in nature, including the hospital environment, where it is frequent the presence of microorganisms that cause hospital infection, especially in immunocompromised patients. To identify and quantify the fungal microbiota in air, the filters of air-conditioners and samples of newborns from the neonatal ICU at University Hospital / HUPA, Maceió, Alagoas. Were held 20 exhibitions of collections totaling 400 disposable Petri dishes containing Sabouraud agar medium plus antibiotic. The plates were placed randomly inside and open the two neonatal ICUs (A and B) for 20 minutes. Also, there were 20 samples of the filters of air-conditioners of neonatal ICUs and conducted the direct examination and culture of clinical samples from newborns. CFU were isolated in 1209, these 675 (55.8%) were isolated CFU before cleaning to be done in the neonatal ICU and 534 (44.2%) CFU obtained after cleaning. Of 697 CFU of the environment of the NICU, 372 were isolated before and 325 after cleaning. Environment B, were obtained of 512 CFU and 303 CFU were obtained before cleaning and 209 after UFC. During the study species of Cladosporium was the most representative in the air and in the filters of air-conditioners. The clinical sample was more representative urine with 79.5%. Among the identified yeast Candida parapsilosis was present in 38.9% of environmental isolates of yeast and Candida albicans was isolated in 20% of clinical samples of newborns. The presence of pathogenic fungi in the two environments of the neonatal ICU and clinical samples of infants, demonstrating the need for constant monitoring to take control of microorganisms in hospital environments, especially in intensive care units where there is the presence of immunocompromised patients prone to develop fungal infections.
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spelling Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, ALMycoflora the environment and neonatal ICU clinical samples from newborns admitted to the University Hospital in Maceió, ALFungiHospital infectionNeonatal ICUCleaningFungosInfecção hospitalarUTI neonatalLimpezaCNPQ::CIENCIAS DA SAUDEThe fungi are being opportunistic, with wide distribution in nature, including the hospital environment, where it is frequent the presence of microorganisms that cause hospital infection, especially in immunocompromised patients. To identify and quantify the fungal microbiota in air, the filters of air-conditioners and samples of newborns from the neonatal ICU at University Hospital / HUPA, Maceió, Alagoas. Were held 20 exhibitions of collections totaling 400 disposable Petri dishes containing Sabouraud agar medium plus antibiotic. The plates were placed randomly inside and open the two neonatal ICUs (A and B) for 20 minutes. Also, there were 20 samples of the filters of air-conditioners of neonatal ICUs and conducted the direct examination and culture of clinical samples from newborns. CFU were isolated in 1209, these 675 (55.8%) were isolated CFU before cleaning to be done in the neonatal ICU and 534 (44.2%) CFU obtained after cleaning. Of 697 CFU of the environment of the NICU, 372 were isolated before and 325 after cleaning. Environment B, were obtained of 512 CFU and 303 CFU were obtained before cleaning and 209 after UFC. During the study species of Cladosporium was the most representative in the air and in the filters of air-conditioners. The clinical sample was more representative urine with 79.5%. Among the identified yeast Candida parapsilosis was present in 38.9% of environmental isolates of yeast and Candida albicans was isolated in 20% of clinical samples of newborns. The presence of pathogenic fungi in the two environments of the neonatal ICU and clinical samples of infants, demonstrating the need for constant monitoring to take control of microorganisms in hospital environments, especially in intensive care units where there is the presence of immunocompromised patients prone to develop fungal infections.Coordenação de Aperfeiçoamento de Pessoal de Nível SuperiorOs fungos que são dispersos pelo ar atmosférico são denominados de fungos anemófilos e são importantes por causar patologias em pacientes imunocomprometidos. Com o objetivo de monitorar a microbiota fúngica do ar, dos filtros dos condicionadores de ar e das amostras clínicas dos recémnascidos internados na UTI neonatal do Hospital Universitário de Maceió - AL foram realizadas coletas de amostras do ar através da técnica de exposição de placas de Petri contendo o meio ágar Sabouraud acrescido de antibiótico. O material sólido dos filtros dos condicionadores de ar foi coletado utilizando um swab esterilizado. E das amostras clínicas dos recém-nascidos foram realizados o exame direto e cultura. Para identificação dos fungos filamentos foram comparadas as características macro e microscópicas e as leveduras foram identificados através da técnica de PCR. Entre as 400 exposições de placas realizadas foram isoladas 1029 UFC, sendo 675 (55,8%) UFC obtidas antes e 534 (44,2%) após a limpeza. Das 40 amostras analisadas dos filtros dos condicionadores de ar foram obtidas 303 UFC, sendo o fungo Cladosporium cladosporioides com maior frequência tanto no ar com 186 (15,4%) UFC como nos filtros dos condicionadores de ar com 77 (37,7%). Não foram isolados fungos filamentosos das amostras clínicas dos recém-nascidos. Entretanto as leveduras Candida albicans, C. tropicalis e C. parapsilosis foram obtidas tanto das amostras do ar como dos recém-nascidos com exceção de C. guilliermondii que foi isolada apenas das amostras do ar.Universidade Federal de AlagoasBrasilPrograma de Pós-Graduação em Ciências da SaúdeUFALSilva Filho, Eurípedes Alves daSILVA FILHO, E. A.Araújo, Maria Anilda dos SantosARAÚJO, M. A. S.Magalhães, Oliane Maria CorreiaMAGALHÃES, O. M. C.Herrmann, Délia Maria de Moura LimaHERRMANN, D. M. M. L.Silva, Luiz Antonio Ferreira daSILVA, L. A. F.Souza, Aryanna Kelly Pinheiro2015-08-25T18:53:04Z2010-07-062015-08-25T18:53:04Z2009-07-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfSOUZA, Aryanna Kelly Pinheiro. Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL. 2009. 111 f. Dissertação (Mestrado em Ciências da Saúde) - Instituto de Ciências Biológicas e da Saúde, Programa de Pós Graduação em Ciências da Saúde, Universidade Federal de Alagoas, Maceió, 2009.http://repositorio.ufal.br/handle/riufal/920porinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da Universidade Federal de Alagoas (UFAL)instname:Universidade Federal de Alagoas (UFAL)instacron:UFAL2018-05-11T12:19:07Zoai:www.repositorio.ufal.br:riufal/920Repositório InstitucionalPUBhttp://www.repositorio.ufal.br/oai/requestri@sibi.ufal.bropendoar:2018-05-11T12:19:07Repositório Institucional da Universidade Federal de Alagoas (UFAL) - Universidade Federal de Alagoas (UFAL)false
dc.title.none.fl_str_mv Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
Mycoflora the environment and neonatal ICU clinical samples from newborns admitted to the University Hospital in Maceió, AL
title Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
spellingShingle Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
Souza, Aryanna Kelly Pinheiro
Fungi
Hospital infection
Neonatal ICU
Cleaning
Fungos
Infecção hospitalar
UTI neonatal
Limpeza
CNPQ::CIENCIAS DA SAUDE
title_short Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
title_full Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
title_fullStr Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
title_full_unstemmed Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
title_sort Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL
author Souza, Aryanna Kelly Pinheiro
author_facet Souza, Aryanna Kelly Pinheiro
author_role author
dc.contributor.none.fl_str_mv Silva Filho, Eurípedes Alves da
SILVA FILHO, E. A.
Araújo, Maria Anilda dos Santos
ARAÚJO, M. A. S.
Magalhães, Oliane Maria Correia
MAGALHÃES, O. M. C.
Herrmann, Délia Maria de Moura Lima
HERRMANN, D. M. M. L.
Silva, Luiz Antonio Ferreira da
SILVA, L. A. F.
dc.contributor.author.fl_str_mv Souza, Aryanna Kelly Pinheiro
dc.subject.por.fl_str_mv Fungi
Hospital infection
Neonatal ICU
Cleaning
Fungos
Infecção hospitalar
UTI neonatal
Limpeza
CNPQ::CIENCIAS DA SAUDE
topic Fungi
Hospital infection
Neonatal ICU
Cleaning
Fungos
Infecção hospitalar
UTI neonatal
Limpeza
CNPQ::CIENCIAS DA SAUDE
description The fungi are being opportunistic, with wide distribution in nature, including the hospital environment, where it is frequent the presence of microorganisms that cause hospital infection, especially in immunocompromised patients. To identify and quantify the fungal microbiota in air, the filters of air-conditioners and samples of newborns from the neonatal ICU at University Hospital / HUPA, Maceió, Alagoas. Were held 20 exhibitions of collections totaling 400 disposable Petri dishes containing Sabouraud agar medium plus antibiotic. The plates were placed randomly inside and open the two neonatal ICUs (A and B) for 20 minutes. Also, there were 20 samples of the filters of air-conditioners of neonatal ICUs and conducted the direct examination and culture of clinical samples from newborns. CFU were isolated in 1209, these 675 (55.8%) were isolated CFU before cleaning to be done in the neonatal ICU and 534 (44.2%) CFU obtained after cleaning. Of 697 CFU of the environment of the NICU, 372 were isolated before and 325 after cleaning. Environment B, were obtained of 512 CFU and 303 CFU were obtained before cleaning and 209 after UFC. During the study species of Cladosporium was the most representative in the air and in the filters of air-conditioners. The clinical sample was more representative urine with 79.5%. Among the identified yeast Candida parapsilosis was present in 38.9% of environmental isolates of yeast and Candida albicans was isolated in 20% of clinical samples of newborns. The presence of pathogenic fungi in the two environments of the neonatal ICU and clinical samples of infants, demonstrating the need for constant monitoring to take control of microorganisms in hospital environments, especially in intensive care units where there is the presence of immunocompromised patients prone to develop fungal infections.
publishDate 2009
dc.date.none.fl_str_mv 2009-07-17
2010-07-06
2015-08-25T18:53:04Z
2015-08-25T18:53:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SOUZA, Aryanna Kelly Pinheiro. Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL. 2009. 111 f. Dissertação (Mestrado em Ciências da Saúde) - Instituto de Ciências Biológicas e da Saúde, Programa de Pós Graduação em Ciências da Saúde, Universidade Federal de Alagoas, Maceió, 2009.
http://repositorio.ufal.br/handle/riufal/920
identifier_str_mv SOUZA, Aryanna Kelly Pinheiro. Microbiota fúngica do ambiente da UTI neonatal e de amostras clínicas dos recém-nascidos internados no Hospital Universitário de Maceió, AL. 2009. 111 f. Dissertação (Mestrado em Ciências da Saúde) - Instituto de Ciências Biológicas e da Saúde, Programa de Pós Graduação em Ciências da Saúde, Universidade Federal de Alagoas, Maceió, 2009.
url http://repositorio.ufal.br/handle/riufal/920
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Universidade Federal de Alagoas
Brasil
Programa de Pós-Graduação em Ciências da Saúde
UFAL
publisher.none.fl_str_mv Universidade Federal de Alagoas
Brasil
Programa de Pós-Graduação em Ciências da Saúde
UFAL
dc.source.none.fl_str_mv reponame:Repositório Institucional da Universidade Federal de Alagoas (UFAL)
instname:Universidade Federal de Alagoas (UFAL)
instacron:UFAL
instname_str Universidade Federal de Alagoas (UFAL)
instacron_str UFAL
institution UFAL
reponame_str Repositório Institucional da Universidade Federal de Alagoas (UFAL)
collection Repositório Institucional da Universidade Federal de Alagoas (UFAL)
repository.name.fl_str_mv Repositório Institucional da Universidade Federal de Alagoas (UFAL) - Universidade Federal de Alagoas (UFAL)
repository.mail.fl_str_mv ri@sibi.ufal.br
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