AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL

Detalhes bibliográficos
Autor(a) principal: Vieira, Jessica
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações do UNIOESTE
Texto Completo: https://tede.unioeste.br/handle/tede/6436
Resumo: Fungi can colonize different substrates and habitats. Atmospheric air is the most used and most successful dispersion environment for these organisms. Airborne fungi, with the ability to establish themselves and contaminate the air, reduce the life quality of the organisms that circulate there. Invasive fungal nosocomial infections have progressively emerged as a relevant source of morbidity and mortality in patients, especially the immunosuppressed patients. The broad spectrum of antimicrobial activity and the great stability led to the use of Wickerhamomyces anomalus as a biocontrol agent, since it could be classified as a low-risk microorganism. In addition, the antimicrobial action of mycocins produced from this yeast in eukaryotic and prokaryotic microorganisms is already proven. Based on this, the aim of the present study was to evaluate the inhibition of airborne fungi isolated in a Neonatal ICU by mycocins produced by W. anomalus. The mycocin containing W. anomalus culture supernatant showed β-glucanase activity of 4.2 U/mg. For fungal monitoring and inhibition test in solid environment, the passive sedimentation technique was used in Petri dishes exposed in the Neonatal ICU, for 15 minutes at 1 m high from the floor and away from the walls. The test consisted of a control plate containing Sabouraud Dextrose Agar, and another composed of culture medium and W. anomalus mycocin supernatant. Then, after incubation, the growth and/or inhibition of the collected fungi was observed. The growth of 15 genera was observed on the control plates: Aureobasidium spp., Curvularia spp., Emmonsia spp., Geotrichun spp., Fusarium spp., Rhizopus spp., Rhodotorula spp., Scedosporium spp., Chrysosporium spp., Trichoderma spp., Alternaria spp., Aspergillus spp., Acremonium spp., Penicillium spp. and Cladosporium spp. Cladosporium spp. was the most incident in the analyzed period, 40% (86 UFC). Meanwhile, on the test plates there was no growth of microorganisms. As a result, we can see the potential of W. anomalus mycocins in the development of new antimicrobial substances.
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spelling Gandra, Rinaldo Ferreirahttp://lattes.cnpq.br/1550489676841150Menezes, Luciana da Silva Ruizhttp://lattes.cnpq.br/6547073557892525Ayala, Thais Sopranihttp://lattes.cnpq.br/5576750114963342http://lattes.cnpq.br/7278005905648317Vieira, Jessica2023-02-09T17:55:33Z2022-11-24Vieira, Jessica. AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL. 2022. 43 f. Dissertação( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, 2022.https://tede.unioeste.br/handle/tede/6436Fungi can colonize different substrates and habitats. Atmospheric air is the most used and most successful dispersion environment for these organisms. Airborne fungi, with the ability to establish themselves and contaminate the air, reduce the life quality of the organisms that circulate there. Invasive fungal nosocomial infections have progressively emerged as a relevant source of morbidity and mortality in patients, especially the immunosuppressed patients. The broad spectrum of antimicrobial activity and the great stability led to the use of Wickerhamomyces anomalus as a biocontrol agent, since it could be classified as a low-risk microorganism. In addition, the antimicrobial action of mycocins produced from this yeast in eukaryotic and prokaryotic microorganisms is already proven. Based on this, the aim of the present study was to evaluate the inhibition of airborne fungi isolated in a Neonatal ICU by mycocins produced by W. anomalus. The mycocin containing W. anomalus culture supernatant showed β-glucanase activity of 4.2 U/mg. For fungal monitoring and inhibition test in solid environment, the passive sedimentation technique was used in Petri dishes exposed in the Neonatal ICU, for 15 minutes at 1 m high from the floor and away from the walls. The test consisted of a control plate containing Sabouraud Dextrose Agar, and another composed of culture medium and W. anomalus mycocin supernatant. Then, after incubation, the growth and/or inhibition of the collected fungi was observed. The growth of 15 genera was observed on the control plates: Aureobasidium spp., Curvularia spp., Emmonsia spp., Geotrichun spp., Fusarium spp., Rhizopus spp., Rhodotorula spp., Scedosporium spp., Chrysosporium spp., Trichoderma spp., Alternaria spp., Aspergillus spp., Acremonium spp., Penicillium spp. and Cladosporium spp. Cladosporium spp. was the most incident in the analyzed period, 40% (86 UFC). Meanwhile, on the test plates there was no growth of microorganisms. As a result, we can see the potential of W. anomalus mycocins in the development of new antimicrobial substances.Os fungos são capazes de colonizar diferentes substratos e habitats, sendo o ar atmosférico o meio de dispersão mais utilizado e mais bem sucedido dentre estes organismos. Os fungos anemófilos, com sua capacidade de se estabelecer e contaminar o ar, diminuem a qualidade de vida dos organismos que ali circulam. As infecções hospitalares fúngicas invasivas têm surgido progressivamente como uma fonte relevante nos quesitos morbidade e mortalidade em pacientes, principalmente aqueles imunossuprimidos. O amplo espectro de atividade antimicrobiana e grande estabilidade levaram o uso de Wickerhamomyces anomalus como um agente de biocontrole, uma vez que poderia ser classificado como um microrganismo de baixo risco. Além disso, já é comprovada a ação antimicrobiana de micocinas produzidas a partir desta levedura em microrganismos eucariotos e procariotos. Partindo disso, o objetivo do presente estudo foi avaliar a inibição de fungos anemófilos isolados em UTI Neonatal por micocinas produzidas por W. anomalus. O sobrenadante da cultura de W. anomalus contendo micocinas mostrou atividade de β-glucanases de 4,2 U/mg. Para o monitoramento fúngico e o teste de inibição em meio sólido, foi utilizada a técnica de sedimentação passiva em placas de Petri expostas na UTI Neonatal, por 15 minutos, a 1 m do chão e distantes das paredes. O teste foi constituído por uma placa controle contendo Ágar Sabouraud Dextrose, e outra composta pelo meio de cultura e sobrenadante de micocinas de W. anomalus. Em seguida, após a incubação, foi observado o crescimento e/ou inibição dos fungos coletados. Observou-se nas placas controle o crescimento de 15 gêneros: Aureobasidium spp., Curvularia spp., Emmonsia spp., Geotrichun spp., Fusarium spp., Rhizopus spp., Rhodotorula spp., Scedosporium spp., Chrysosporium spp., Trichoderma spp., Alternaria spp., Aspergillus spp., Acremonium spp., Penicillium spp. e Cladosporium spp, sendo o Cladosporium spp. o mais incidente no período analisado, 40% (86 UFC), enquanto nas placas teste não houve o crescimento de microrganismos. Sendo assim, podemos notar o potencial das micocinas de W. anomalus no desenvolvimento de novas substâncias antimicrobianasSubmitted by Edineia Teixeira (edineia.teixeira@unioeste.br) on 2023-02-09T17:55:33Z No. of bitstreams: 2 JESSICA_ VIEIRA.2022.pdf: 520853 bytes, checksum: d4e873a4cd617c0a9c2f07f8473d0ab0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2023-02-09T17:55:33Z (GMT). No. of bitstreams: 2 JESSICA_ VIEIRA.2022.pdf: 520853 bytes, checksum: d4e873a4cd617c0a9c2f07f8473d0ab0 (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2022-11-24application/pdfpor6588633818200016417500Universidade Estadual do Oeste do ParanáCascavelPrograma de Pós-Graduação em Ciências FarmacêuticasUNIOESTEBrasilCentro de Ciências Médicas e Farmacêuticashttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessWickerhamomyces anomalusMicocinasFungos anemófilosUTI neonatalWickerhamomyces anomalusMycocinsAirborne fungiNeonatal ICUCIÊNCIAS FARMACEUTICASAÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATALINHIBITORY ACTION OF MYCOCINS ON AIRBORNE FUNGI ISOLATED FROM NEONATAL ICUinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesis7878055067573953101600600-8940439713387849267reponame:Biblioteca Digital de Teses e Dissertações do UNIOESTEinstname:Universidade Estadual do Oeste do Paraná (UNIOESTE)instacron:UNIOESTEORIGINALJESSICA_ VIEIRA.2022.pdfJESSICA_ VIEIRA.2022.pdfapplication/pdf520853http://tede.unioeste.br:8080/tede/bitstream/tede/6436/5/JESSICA_+VIEIRA.2022.pdfd4e873a4cd617c0a9c2f07f8473d0ab0MD55CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
dc.title.alternative.eng.fl_str_mv INHIBITORY ACTION OF MYCOCINS ON AIRBORNE FUNGI ISOLATED FROM NEONATAL ICU
title AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
spellingShingle AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
Vieira, Jessica
Wickerhamomyces anomalus
Micocinas
Fungos anemófilos
UTI neonatal
Wickerhamomyces anomalus
Mycocins
Airborne fungi
Neonatal ICU
CIÊNCIAS FARMACEUTICAS
title_short AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
title_full AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
title_fullStr AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
title_full_unstemmed AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
title_sort AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL
author Vieira, Jessica
author_facet Vieira, Jessica
author_role author
dc.contributor.advisor1.fl_str_mv Gandra, Rinaldo Ferreira
dc.contributor.advisor1Lattes.fl_str_mv http://lattes.cnpq.br/1550489676841150
dc.contributor.referee1.fl_str_mv Menezes, Luciana da Silva Ruiz
dc.contributor.referee1Lattes.fl_str_mv http://lattes.cnpq.br/6547073557892525
dc.contributor.referee2.fl_str_mv Ayala, Thais Soprani
dc.contributor.referee2Lattes.fl_str_mv http://lattes.cnpq.br/5576750114963342
dc.contributor.authorLattes.fl_str_mv http://lattes.cnpq.br/7278005905648317
dc.contributor.author.fl_str_mv Vieira, Jessica
contributor_str_mv Gandra, Rinaldo Ferreira
Menezes, Luciana da Silva Ruiz
Ayala, Thais Soprani
dc.subject.por.fl_str_mv Wickerhamomyces anomalus
Micocinas
Fungos anemófilos
UTI neonatal
topic Wickerhamomyces anomalus
Micocinas
Fungos anemófilos
UTI neonatal
Wickerhamomyces anomalus
Mycocins
Airborne fungi
Neonatal ICU
CIÊNCIAS FARMACEUTICAS
dc.subject.eng.fl_str_mv Wickerhamomyces anomalus
Mycocins
Airborne fungi
Neonatal ICU
dc.subject.cnpq.fl_str_mv CIÊNCIAS FARMACEUTICAS
description Fungi can colonize different substrates and habitats. Atmospheric air is the most used and most successful dispersion environment for these organisms. Airborne fungi, with the ability to establish themselves and contaminate the air, reduce the life quality of the organisms that circulate there. Invasive fungal nosocomial infections have progressively emerged as a relevant source of morbidity and mortality in patients, especially the immunosuppressed patients. The broad spectrum of antimicrobial activity and the great stability led to the use of Wickerhamomyces anomalus as a biocontrol agent, since it could be classified as a low-risk microorganism. In addition, the antimicrobial action of mycocins produced from this yeast in eukaryotic and prokaryotic microorganisms is already proven. Based on this, the aim of the present study was to evaluate the inhibition of airborne fungi isolated in a Neonatal ICU by mycocins produced by W. anomalus. The mycocin containing W. anomalus culture supernatant showed β-glucanase activity of 4.2 U/mg. For fungal monitoring and inhibition test in solid environment, the passive sedimentation technique was used in Petri dishes exposed in the Neonatal ICU, for 15 minutes at 1 m high from the floor and away from the walls. The test consisted of a control plate containing Sabouraud Dextrose Agar, and another composed of culture medium and W. anomalus mycocin supernatant. Then, after incubation, the growth and/or inhibition of the collected fungi was observed. The growth of 15 genera was observed on the control plates: Aureobasidium spp., Curvularia spp., Emmonsia spp., Geotrichun spp., Fusarium spp., Rhizopus spp., Rhodotorula spp., Scedosporium spp., Chrysosporium spp., Trichoderma spp., Alternaria spp., Aspergillus spp., Acremonium spp., Penicillium spp. and Cladosporium spp. Cladosporium spp. was the most incident in the analyzed period, 40% (86 UFC). Meanwhile, on the test plates there was no growth of microorganisms. As a result, we can see the potential of W. anomalus mycocins in the development of new antimicrobial substances.
publishDate 2022
dc.date.issued.fl_str_mv 2022-11-24
dc.date.accessioned.fl_str_mv 2023-02-09T17:55:33Z
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dc.identifier.citation.fl_str_mv Vieira, Jessica. AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL. 2022. 43 f. Dissertação( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, 2022.
dc.identifier.uri.fl_str_mv https://tede.unioeste.br/handle/tede/6436
identifier_str_mv Vieira, Jessica. AÇÃO INIBITÓRIA DE MICOCINAS SOBRE FUNGOS ANEMÓFILOS ISOLADOS DE UTI NEONATAL. 2022. 43 f. Dissertação( Mestrado em Ciências Farmacêuticas) - Universidade Estadual do Oeste do Paraná, 2022.
url https://tede.unioeste.br/handle/tede/6436
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dc.relation.confidence.fl_str_mv 600
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dc.publisher.department.fl_str_mv Centro de Ciências Médicas e Farmacêuticas
publisher.none.fl_str_mv Universidade Estadual do Oeste do Paraná
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