Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano

Detalhes bibliográficos
Autor(a) principal: Silva, Warleson Parente da
Data de Publicação: 2022
Tipo de documento: Trabalho de conclusão de curso
Idioma: por
Título da fonte: Repositório Institucional da UFAM
Texto Completo: http://riu.ufam.edu.br/handle/prefix/6422
Resumo: Water is an indispensable and essential chemical element for the maintenance of life, which makes it an important component in public health. On the other hand, water can be considered as a route for the transmission of diseases, especially by pathogenic microorganisms that may or may not be resistant to antibiotics. Currently, it is known that microorganisms, especially antibiotics, are considered a major threat to modern treatments, claiming the lives of millions of people around the world. In this scenario, the search for new alternatives with the potential to alleviate this problem has been increasing. Thus, the components from natural products are shown to be potent and promising in combating the spread of bacterial strains to humans, among which we can highlight the essential oil of Syzigium aromaticum, which has been shown to be an antimicrobial agent. Therefore, the present work aims to evaluate the antimicrobial activity of Syzigium aromaticum against bacterial strains from drinking water. Bacterial strains were isolated from samples of water for human consumption or from the larger project entitled “IMPACT OF CLIMATE EVENTS (FLOOD AND DROUGHT) ON THE QUALITY OF WATER FOR CONSUMPTION OF INDIVIDUALS RESULTING IN FLOATING HOUSES IN THE AMAZON”. The effects of the essential oil were achieved through the Diffusion Disk Technique in Adaptation with the Antimicrobial Sensitivity Test. The results show that the maximum essential activity, in all oil sizes, are all antibacterials 2 with diameters 22 and 2 mm. (C13_Tefé), Vibrio spp. (C1_Coari) and Salmonella spp. (C5_Tefé). In addition, it showed remarkable activity to those who showed resistance to Chloramphenicol (30 µg), especially Vibrio spp. (C13_Tefé) and Vibrio spp (C4_Tefé) with diameters of 24 and 23 mm respectively. Our unique findings of such a component as a valid alternative for the treatment of recipients, specifically recognized by bacterial Vibrio spp. and Salmonella spp. present in water for human consumption. However, additional studies need to be performed to elucidate the essential oil cytotoxicity profile in experimental models. Keywords: Clove, antimicrobial effects, drinking water, pathogenic microorganisms.
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spelling Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humanoCravo da índiaefeitos antimicrobianoCIENCIAS BIOLOGICASCravo da IndiaWater is an indispensable and essential chemical element for the maintenance of life, which makes it an important component in public health. On the other hand, water can be considered as a route for the transmission of diseases, especially by pathogenic microorganisms that may or may not be resistant to antibiotics. Currently, it is known that microorganisms, especially antibiotics, are considered a major threat to modern treatments, claiming the lives of millions of people around the world. In this scenario, the search for new alternatives with the potential to alleviate this problem has been increasing. Thus, the components from natural products are shown to be potent and promising in combating the spread of bacterial strains to humans, among which we can highlight the essential oil of Syzigium aromaticum, which has been shown to be an antimicrobial agent. Therefore, the present work aims to evaluate the antimicrobial activity of Syzigium aromaticum against bacterial strains from drinking water. Bacterial strains were isolated from samples of water for human consumption or from the larger project entitled “IMPACT OF CLIMATE EVENTS (FLOOD AND DROUGHT) ON THE QUALITY OF WATER FOR CONSUMPTION OF INDIVIDUALS RESULTING IN FLOATING HOUSES IN THE AMAZON”. The effects of the essential oil were achieved through the Diffusion Disk Technique in Adaptation with the Antimicrobial Sensitivity Test. The results show that the maximum essential activity, in all oil sizes, are all antibacterials 2 with diameters 22 and 2 mm. (C13_Tefé), Vibrio spp. (C1_Coari) and Salmonella spp. (C5_Tefé). In addition, it showed remarkable activity to those who showed resistance to Chloramphenicol (30 µg), especially Vibrio spp. (C13_Tefé) and Vibrio spp (C4_Tefé) with diameters of 24 and 23 mm respectively. Our unique findings of such a component as a valid alternative for the treatment of recipients, specifically recognized by bacterial Vibrio spp. and Salmonella spp. present in water for human consumption. However, additional studies need to be performed to elucidate the essential oil cytotoxicity profile in experimental models. Keywords: Clove, antimicrobial effects, drinking water, pathogenic microorganisms.Á água é um elemento químico indispensável e essencial para a manutenção da vida, o que a torna um componente importante na saúde pública. Em contrapartida, a água pode ser considerada como uma via para a transmissão de doenças, especialmente, aquelas causadas por microrganismos patogênicos que podem ou não apresentar resistência a antibióticos. Atualmente, sabe-se que microrganismos, em especial, resistentes a antibióticos são considerados uma grande ameaça para os tratamentos modernos, ceifando a vida de milhões de pessoas ao redor do mundo. Nesse cenário, tem sido crescente a busca por novas alternativas com potencial de amenizar tal problemática. Dessa forma, os componentes provenientes dos produtos naturais, mostram-se promissores no combate a disseminação de cepas bacterinas patogênicas ao homem, dentre os quais podemos destacar, o óleo essencial de Syzigium aromaticum, que têm se demostrando como um potente agente antimicrobiano. Portanto, o presente trabalho tem como objetivo avaliar a atividade antimicrobiana de Syzigium aromaticum frente a cepas bacterianas oriundas de água para consumo humano. As cepas bacterianas foram isoladas a partir de amostras de água para consumo humano oriundas do projeto maior intitulado “IMPACTO DOS EVENTOS CLIMÁTICOS (INUNDAÇÕES E SECA) NA QUALIDADE DA ÁGUA DE CONSUMO DE INDIVÍDUOS MORADORES DE CASAS FLUTUANTES NO AMAZONAS”. Os efeitos do óleo essencial foram avaliados por meio da Técnica de Difusão em Disco em adaptação com o Teste de Sensibilidade aos antimicrobianos. Os resultados mostram que o óleo essencial, em todas as concentrações, apresentou atividade antibacteriana satisfatória com diâmetros máximo de 26, 22 e 21 mm para Salmonela spp. (C13_Tefé), Vibrio spp. (C1_Coari) e Salmonela spp. (C5_Tefé). Ademais, apresentou atividade notável aos que apresentaram resistência ao Cloranfenicol (30 µg), em especial, Vibrio spp. (C13_Tefé) e Vibrio spp (C4_Tefé) com diâmetro de 24 e 23 mm respectivamente. Nossos achados sugerem tal componente como uma possível alternativa válida para tratar infecções, especialmente, aquelas causadas por cepas bacterianas de Vibrio spp. e Salmonela spp. presentes na água para consumo humano. No entanto, estudos adicionais precisam ser realizados para elucidar o perfil de citotoxicidade do óleo essencial em modelos experimentais. Palavras - chave: Cravo da índia, efeitos antimicrobiano, água para consumo humano, microrganismos patogênicos.1NãoBrasilISB - Instituto de Saúde e Biotecnologia (Coari)COARIBiotecnologia - Bacharelado - CoariChamy, Michel Nasser Correa Limahttp://lattes.cnpq.br/5334792132991651Nasser Correa Lima Chamy, MichelKazumy de Lima Yamaguchi, KlenicyRosana da Silva Souza, GiulianaLaboratório de MicrobiologiaSilva, Warleson Parente da2022-10-04T17:52:55Z2022-10-032022-10-04T17:52:55Z2022-09-22info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesishttp://riu.ufam.edu.br/handle/prefix/6422porAUSTIN. C, B. et al. Vibrio spp. Infections. Nature Reviews Disease Primers. 2018. Weymouth, v. 4, n. 1, p. 1-19, jun./dec. 2018. ASBAHANI. A. E. et al. Essential oils: From extraction to encapsulation. International Journal of Pharmaceutics. Lyon, v. 483, n.2, p. 220-243, abr. 2015. BAYOT. M, l.; BRAGG.; B, N. Antimicrobial Susceptibility Testing. StatPearls Publishing, Florida, oct. 2021. BAUER. A, W. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Patho. v. 45, n. 4, p. 493-6, 1966. BARTOLONI. A. et al. Antibiotic resistance in a very remote Amazonas community. Int J Antimicrob Agents. Firenza, v. 33, n. 2, p. 125-129, ver. 2008. BRASIL. PORTARIA GM/MS Nº 888, DE 4 DE MAIO DE 2021. Ministério da saúde. Gabinete do ministro. 2021. Clinical and Laboratory Standards Institute – CLSI, 2020. DEVI. K, P. et al. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J Ethnopharmacol. v. 130, n. 1, p. 107-115, jul. 2010. CASSINI. A. et al. Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Economic Area in 2015: a population-level modelling analysis. Lancet Infect Dis. Solna, v. 19, n.1, p. 56-66, p. 107-115, jan. 2018. DIB. J. et al. Isolation of bacteria from remote high altitude Andean lakes able to grow in the presence of antibiotics. Recent Pat Antiinfect Drug Discov, v. 4, n. 1, p. 66-76, jan. 2009. FRAZÃO. P.; PERES. M, A.; CURY. J, A. Qualidade da água para consumo humano e concentração de fluoreto. Rev Saúde Pública. São Paulo. v. 45, n. 5, p. 964-73, ago. 2011. FRIEDMAN. N. D.; TEMKIN. E.; CARMELI. Y. The negative impact of antibiotic resistance. Clin Microbiol Infect, Aviv, v. 22, n. 5, p. 416-422, mai. 2016. GOZÁLEZ. J. N. H. et al. Clove Essential Oil (Syzygium aromaticum L. Myrtaceae): Extraction, Chemical Composition, Food Applications, and Essential Bioactivity for Human Health. Molecules. Guadalajara, v. 26, n. 21, p. 2-25, ago./ oct., 2021. LARSON. A. et al. Antibiotic-Resistant Escherichia coli in Drinking Water Samples from Rural Andean Households in Cajamarca. The American Journal of Tropical Medicine and Hygiene. Lima, v. 100 n. 6, set./ ferv. 2019. LEE. S. et al. Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites. Frontiers of Agriculture in China. Kuala Terengganu. v. 3, n. 3, p.332-336, jan./Feb. 2009. MARQUEZI. M, C.; GALLO. C, R.; DIAS. C, T, S. Comparação entre métodos para a análise de coliformes totais e E. coli em amostras de água. Rev Inst Adolfo Lutz. São Paulo, v. 69, n. 3, p. 291-6, set. 2010. Organização Mundial de Saúde. (2022, 21 de março). Disponível em: https://www.who.int/news-room/fact-sheets/detail/drinking-water#:~:text=Contaminated%20water%20and%20poor%20sanitation,hepatitis%20A%2C%20typhoid%20and%20polio. Acesso em: 18 de agosto, 2022. POPA. G, L.; PAPA. M, L. Salmonella spp. infection - a continuous threat worldwide. GERMS. Bucharest, v. 12, n. 2, p.88-96, Ago./ Ferv. 2021. RATES. S, M, K. Plants as a source of drugs. Toxicon, Rio Grande do Sul, v. 39, n. 5, p. 603-613, mai, 2001. RADÜNZ. M. et al. Antimicrobial and antioxidant activity of unencapsulated and encapsulated clove (Syzygium aromaticum, L.) essential oil. Food Chemistry. Pelotas, v. 276, p. 180-186, mar. 2019. ROSSI. F. The Challenges of Antimicrobial Resistance in Brazil. Clinical Infectious Diseases. São Paulo, v. 52, n. 9, p.1138-1143, mai. 2011. THOROSKI. J.; BLANK. B.; BILIADERIS. C. Eugenol Induced Inhibition of Extracellular Enzyme Production by Bacillus subtilis. J Food Pro. Manitoba, v. 52, n. 6, p. 399-403, jun. 1989. KAKOULLIS. L. et al. Mechanisms of Antibiotic Resistance in Important Gram-Positive and Gram-Negative Pathogens and Novel Antibiotic Solutions. Antibiotics, Patras, v. 10, n. 4, p. 415, Apr. 2021. VEERESHAM. S. Natural products derived from plants as a source of drugs. Journal of Advanced Pharmaceutical Technology & Research. Andhra Pradesh, v. 3, n. 4, p. 200-201, dez. 2011. XU. J, G. et al. Chemical Composition, Antibacterial Properties and Mechanism of Action of Essential Oil from Clove Buds against Staphylococcus aureus. Molecules. Linfen, v. 21, n. 9, p. 1-194, 2016. WINSKA. K. et al. settings Open AccessReview Essential Oils as Antimicrobial Agents—Myth or Real Alternative?. Molecules. Norwida, v. 24, n. 11, ab./ jun. 2019. WONGSAWAN. K. et al. Bactericidal Effect of Clove Oil against Multidrug-Resistant Streptococcus suis Isolated from Human Patients and Slaughtered Pigs. Pathogens, Chiang, v. 9, n. 1, p.2-12, dec. 2020. WENDAKOON. C.; SAKAGUCHI. C. Combined Effect of Sodium Chloride and Clove on Growth and Biogenic Amine Formation of Enterobacter aerogenes in Mackerel Muscle Extract. J Food Prot. Kyoto, v.56, n. 5, p. 410-413, mai. 1993.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFAMinstname:Universidade Federal do Amazonas (UFAM)instacron:UFAM2022-10-04T17:53:38Zoai:localhost:prefix/6422Repositório InstitucionalPUBhttp://riu.ufam.edu.br/oai/requestopendoar:2022-10-04T17:53:38Repositório Institucional da UFAM - Universidade Federal do Amazonas (UFAM)false
dc.title.none.fl_str_mv Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
title Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
spellingShingle Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
Silva, Warleson Parente da
Cravo da índia
efeitos antimicrobiano
CIENCIAS BIOLOGICAS
Cravo da India
title_short Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
title_full Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
title_fullStr Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
title_full_unstemmed Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
title_sort Avaliação da atividade antimicrobiana do óleo essencial do cravo da índia (Syzigium aromaticum) frente a cepas bacterianas oriundas da água para consumo humano
author Silva, Warleson Parente da
author_facet Silva, Warleson Parente da
author_role author
dc.contributor.none.fl_str_mv Chamy, Michel Nasser Correa Lima
http://lattes.cnpq.br/5334792132991651
Nasser Correa Lima Chamy, Michel
Kazumy de Lima Yamaguchi, Klenicy
Rosana da Silva Souza, Giuliana
Laboratório de Microbiologia
dc.contributor.author.fl_str_mv Silva, Warleson Parente da
dc.subject.por.fl_str_mv Cravo da índia
efeitos antimicrobiano
CIENCIAS BIOLOGICAS
Cravo da India
topic Cravo da índia
efeitos antimicrobiano
CIENCIAS BIOLOGICAS
Cravo da India
description Water is an indispensable and essential chemical element for the maintenance of life, which makes it an important component in public health. On the other hand, water can be considered as a route for the transmission of diseases, especially by pathogenic microorganisms that may or may not be resistant to antibiotics. Currently, it is known that microorganisms, especially antibiotics, are considered a major threat to modern treatments, claiming the lives of millions of people around the world. In this scenario, the search for new alternatives with the potential to alleviate this problem has been increasing. Thus, the components from natural products are shown to be potent and promising in combating the spread of bacterial strains to humans, among which we can highlight the essential oil of Syzigium aromaticum, which has been shown to be an antimicrobial agent. Therefore, the present work aims to evaluate the antimicrobial activity of Syzigium aromaticum against bacterial strains from drinking water. Bacterial strains were isolated from samples of water for human consumption or from the larger project entitled “IMPACT OF CLIMATE EVENTS (FLOOD AND DROUGHT) ON THE QUALITY OF WATER FOR CONSUMPTION OF INDIVIDUALS RESULTING IN FLOATING HOUSES IN THE AMAZON”. The effects of the essential oil were achieved through the Diffusion Disk Technique in Adaptation with the Antimicrobial Sensitivity Test. The results show that the maximum essential activity, in all oil sizes, are all antibacterials 2 with diameters 22 and 2 mm. (C13_Tefé), Vibrio spp. (C1_Coari) and Salmonella spp. (C5_Tefé). In addition, it showed remarkable activity to those who showed resistance to Chloramphenicol (30 µg), especially Vibrio spp. (C13_Tefé) and Vibrio spp (C4_Tefé) with diameters of 24 and 23 mm respectively. Our unique findings of such a component as a valid alternative for the treatment of recipients, specifically recognized by bacterial Vibrio spp. and Salmonella spp. present in water for human consumption. However, additional studies need to be performed to elucidate the essential oil cytotoxicity profile in experimental models. Keywords: Clove, antimicrobial effects, drinking water, pathogenic microorganisms.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-04T17:52:55Z
2022-10-03
2022-10-04T17:52:55Z
2022-09-22
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dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv AUSTIN. C, B. et al. Vibrio spp. Infections. Nature Reviews Disease Primers. 2018. Weymouth, v. 4, n. 1, p. 1-19, jun./dec. 2018. ASBAHANI. A. E. et al. Essential oils: From extraction to encapsulation. International Journal of Pharmaceutics. Lyon, v. 483, n.2, p. 220-243, abr. 2015. BAYOT. M, l.; BRAGG.; B, N. Antimicrobial Susceptibility Testing. StatPearls Publishing, Florida, oct. 2021. BAUER. A, W. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Patho. v. 45, n. 4, p. 493-6, 1966. BARTOLONI. A. et al. Antibiotic resistance in a very remote Amazonas community. Int J Antimicrob Agents. Firenza, v. 33, n. 2, p. 125-129, ver. 2008. BRASIL. PORTARIA GM/MS Nº 888, DE 4 DE MAIO DE 2021. Ministério da saúde. Gabinete do ministro. 2021. Clinical and Laboratory Standards Institute – CLSI, 2020. DEVI. K, P. et al. Eugenol (an essential oil of clove) acts as an antibacterial agent against Salmonella typhi by disrupting the cellular membrane. J Ethnopharmacol. v. 130, n. 1, p. 107-115, jul. 2010. CASSINI. A. et al. Attributable deaths and disability-adjusted life-years caused by infections with antibiotic-resistant bacteria in the EU and the European Economic Area in 2015: a population-level modelling analysis. Lancet Infect Dis. Solna, v. 19, n.1, p. 56-66, p. 107-115, jan. 2018. DIB. J. et al. Isolation of bacteria from remote high altitude Andean lakes able to grow in the presence of antibiotics. Recent Pat Antiinfect Drug Discov, v. 4, n. 1, p. 66-76, jan. 2009. FRAZÃO. P.; PERES. M, A.; CURY. J, A. Qualidade da água para consumo humano e concentração de fluoreto. Rev Saúde Pública. São Paulo. v. 45, n. 5, p. 964-73, ago. 2011. FRIEDMAN. N. D.; TEMKIN. E.; CARMELI. Y. The negative impact of antibiotic resistance. Clin Microbiol Infect, Aviv, v. 22, n. 5, p. 416-422, mai. 2016. GOZÁLEZ. J. N. H. et al. Clove Essential Oil (Syzygium aromaticum L. Myrtaceae): Extraction, Chemical Composition, Food Applications, and Essential Bioactivity for Human Health. Molecules. Guadalajara, v. 26, n. 21, p. 2-25, ago./ oct., 2021. LARSON. A. et al. Antibiotic-Resistant Escherichia coli in Drinking Water Samples from Rural Andean Households in Cajamarca. The American Journal of Tropical Medicine and Hygiene. Lima, v. 100 n. 6, set./ ferv. 2019. LEE. S. et al. Chemical composition and antimicrobial activity of the essential oil of Syzygium aromaticum flower bud (Clove) against fish systemic bacteria isolated from aquaculture sites. Frontiers of Agriculture in China. Kuala Terengganu. v. 3, n. 3, p.332-336, jan./Feb. 2009. MARQUEZI. M, C.; GALLO. C, R.; DIAS. C, T, S. Comparação entre métodos para a análise de coliformes totais e E. coli em amostras de água. Rev Inst Adolfo Lutz. São Paulo, v. 69, n. 3, p. 291-6, set. 2010. Organização Mundial de Saúde. (2022, 21 de março). Disponível em: https://www.who.int/news-room/fact-sheets/detail/drinking-water#:~:text=Contaminated%20water%20and%20poor%20sanitation,hepatitis%20A%2C%20typhoid%20and%20polio. Acesso em: 18 de agosto, 2022. POPA. G, L.; PAPA. M, L. Salmonella spp. infection - a continuous threat worldwide. GERMS. Bucharest, v. 12, n. 2, p.88-96, Ago./ Ferv. 2021. RATES. S, M, K. Plants as a source of drugs. Toxicon, Rio Grande do Sul, v. 39, n. 5, p. 603-613, mai, 2001. RADÜNZ. M. et al. Antimicrobial and antioxidant activity of unencapsulated and encapsulated clove (Syzygium aromaticum, L.) essential oil. Food Chemistry. Pelotas, v. 276, p. 180-186, mar. 2019. ROSSI. F. The Challenges of Antimicrobial Resistance in Brazil. Clinical Infectious Diseases. São Paulo, v. 52, n. 9, p.1138-1143, mai. 2011. THOROSKI. J.; BLANK. B.; BILIADERIS. C. Eugenol Induced Inhibition of Extracellular Enzyme Production by Bacillus subtilis. J Food Pro. Manitoba, v. 52, n. 6, p. 399-403, jun. 1989. KAKOULLIS. L. et al. Mechanisms of Antibiotic Resistance in Important Gram-Positive and Gram-Negative Pathogens and Novel Antibiotic Solutions. Antibiotics, Patras, v. 10, n. 4, p. 415, Apr. 2021. VEERESHAM. S. Natural products derived from plants as a source of drugs. Journal of Advanced Pharmaceutical Technology & Research. Andhra Pradesh, v. 3, n. 4, p. 200-201, dez. 2011. XU. J, G. et al. Chemical Composition, Antibacterial Properties and Mechanism of Action of Essential Oil from Clove Buds against Staphylococcus aureus. Molecules. Linfen, v. 21, n. 9, p. 1-194, 2016. WINSKA. K. et al. settings Open AccessReview Essential Oils as Antimicrobial Agents—Myth or Real Alternative?. Molecules. Norwida, v. 24, n. 11, ab./ jun. 2019. WONGSAWAN. K. et al. Bactericidal Effect of Clove Oil against Multidrug-Resistant Streptococcus suis Isolated from Human Patients and Slaughtered Pigs. Pathogens, Chiang, v. 9, n. 1, p.2-12, dec. 2020. WENDAKOON. C.; SAKAGUCHI. C. Combined Effect of Sodium Chloride and Clove on Growth and Biogenic Amine Formation of Enterobacter aerogenes in Mackerel Muscle Extract. J Food Prot. Kyoto, v.56, n. 5, p. 410-413, mai. 1993.
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