Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/16650 |
Resumo: | Texto completo: acesso restrito. p. 1575–1584 |
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Weinkopff, TiffanyMariotto, AnitaSimon, GregoireTorre, Yazmin Hauyon-LaAuderset, FlorianeSchuster, SteffenZangger, HarounFasel, NicolasBarral, Aldina Maria PradoCottier, Fabienne TacchiniWeinkopff, TiffanyMariotto, AnitaSimon, GregoireTorre, Yazmin Hauyon-LaAuderset, FlorianeSchuster, SteffenZangger, HarounFasel, NicolasBarral, Aldina Maria PradoCottier, Fabienne Tacchini2014-11-24T15:20:28Z20130019-9567http://repositorio.ufba.br/ri/handle/ri/16650v. 81, n. 5Texto completo: acesso restrito. p. 1575–1584Infection with Leishmania braziliensis causes cutaneous or mucocutaneous leismaniasis in humans. Toll-like receptor 9 (TLR9) expression has been found in granulomas of lesions in L. braziliensis-infected individuals. L. braziliensis inoculation in mice induces very small lesions that are self-healing, whereas deficiency in the TLR adaptor molecule, MyD88, renders mice susceptible to infection. The TLR involved has not been identified, prompting us to investigate if TLR9 triggering by the parasite contributes to the strong resistance to infection observed in L. braziliensis-inoculated mice. The parasites activated wild-type (WT) dendritic cells (DCs) in vitro but not DCs derived from TLR9−/− mice. TLR9−/− mice inoculated with L. braziliensis exhibited a transient susceptibility characterized by increased lesion size and parasite burden compared to those of WT mice. Surprisingly, elevated levels of gamma interferon (IFN-γ) were measured at the site of infection and in draining lymph node T cells of TLR9−/− mice at the peak of susceptibility, suggesting that unlike observations in vitro, the parasite could induce DC activation leading to the development of Th1 cells in the absence of TLR9 expression. Taken together, these data show that TLR9 signaling is important for the early control of lesion development and parasite burden but is dispensable for the differentiation of Th1 cells secreting IFN-γ, and the high levels of this cytokine are not sufficient to control early parasite replication following L. braziliensis infection.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-11-24T14:00:17Z No. of bitstreams: 1 Tiffany Weinkopff.pdf: 1537418 bytes, checksum: 51a9b9cea1b36b6c8fd1cde41e93be9b (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-11-24T15:20:28Z (GMT) No. of bitstreams: 1 Tiffany Weinkopff.pdf: 1537418 bytes, checksum: 51a9b9cea1b36b6c8fd1cde41e93be9b (MD5)Made available in DSpace on 2014-11-24T15:20:28Z (GMT). No. of bitstreams: 1 Tiffany Weinkopff.pdf: 1537418 bytes, checksum: 51a9b9cea1b36b6c8fd1cde41e93be9b (MD5) Previous issue date: 2013http://dx.doi.org/ 10.1128/IAI.01401-12reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania braziliensisLeishmaniasis MucocutaneousLeishmaniasis CutaneousTh1 CellsRole of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis InfectionInfection and Immunityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALTiffany Weinkopff.pdfTiffany Weinkopff.pdfapplication/pdf1537418https://repositorio.ufba.br/bitstream/ri/16650/1/Tiffany%20Weinkopff.pdf51a9b9cea1b36b6c8fd1cde41e93be9bMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16650/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTTiffany Weinkopff.pdf.txtTiffany Weinkopff.pdf.txtExtracted texttext/plain56871https://repositorio.ufba.br/bitstream/ri/16650/3/Tiffany%20Weinkopff.pdf.txte2e1f5f5d9bbdd87460481464bd43cfcMD53ri/166502022-08-08 13:20:41.485oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:20:41Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| dc.title.alternative.pt_BR.fl_str_mv |
Infection and Immunity |
| title |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| spellingShingle |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection Weinkopff, Tiffany Leishmania braziliensis Leishmaniasis Mucocutaneous Leishmaniasis Cutaneous Th1 Cells |
| title_short |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| title_full |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| title_fullStr |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| title_full_unstemmed |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| title_sort |
Role of Toll-Like Receptor 9 Signaling in Experimental Leishmania braziliensis Infection |
| author |
Weinkopff, Tiffany |
| author_facet |
Weinkopff, Tiffany Mariotto, Anita Simon, Gregoire Torre, Yazmin Hauyon-La Auderset, Floriane Schuster, Steffen Zangger, Haroun Fasel, Nicolas Barral, Aldina Maria Prado Cottier, Fabienne Tacchini |
| author_role |
author |
| author2 |
Mariotto, Anita Simon, Gregoire Torre, Yazmin Hauyon-La Auderset, Floriane Schuster, Steffen Zangger, Haroun Fasel, Nicolas Barral, Aldina Maria Prado Cottier, Fabienne Tacchini |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Weinkopff, Tiffany Mariotto, Anita Simon, Gregoire Torre, Yazmin Hauyon-La Auderset, Floriane Schuster, Steffen Zangger, Haroun Fasel, Nicolas Barral, Aldina Maria Prado Cottier, Fabienne Tacchini Weinkopff, Tiffany Mariotto, Anita Simon, Gregoire Torre, Yazmin Hauyon-La Auderset, Floriane Schuster, Steffen Zangger, Haroun Fasel, Nicolas Barral, Aldina Maria Prado Cottier, Fabienne Tacchini |
| dc.subject.por.fl_str_mv |
Leishmania braziliensis Leishmaniasis Mucocutaneous Leishmaniasis Cutaneous Th1 Cells |
| topic |
Leishmania braziliensis Leishmaniasis Mucocutaneous Leishmaniasis Cutaneous Th1 Cells |
| description |
Texto completo: acesso restrito. p. 1575–1584 |
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2013 |
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2013 |
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2014-11-24T15:20:28Z |
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0019-9567 |
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v. 81, n. 5 |
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0019-9567 v. 81, n. 5 |
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