Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Oliveira, Fabiano
Data de Publicação: 2006
Outros Autores: Anderson, Jennifer M., Kamhawi, Shaden, Mans, Ben J., Reynoso, David, Seitz, Amy E., Lawyer, Phillip, Garfield, Mark, Pham, MyVan, Valenzuela, Jesus G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13405
Resumo: p.1-23
id UFBA-2_15fd8a8f3fdb69b42ed122e6e692308d
oai_identifier_str oai:repositorio.ufba.br:ri/13405
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Oliveira, FabianoAnderson, Jennifer M.Kamhawi, ShadenMans, Ben J.Reynoso, DavidSeitz, Amy E.Lawyer, PhillipGarfield, MarkPham, MyVanValenzuela, Jesus G.Oliveira, FabianoAnderson, Jennifer M.Kamhawi, ShadenMans, Ben J.Reynoso, DavidSeitz, Amy E.Lawyer, PhillipGarfield, MarkPham, MyVanValenzuela, Jesus G.2013-10-31T17:44:53Z2013-10-31T17:44:53Z20061471-2164http://repositorio.ufba.br/ri/handle/ri/13405v.7 n. 52p.1-23Background: Immune responses to sandfly saliva have been shown to protect animals against Leishmania infection. Yet very little is known about the molecular characteristics of salivary proteins from different sandflies, particularly from vectors transmitting visceral leishmaniasis, the fatal form of the disease. Further knowledge of the repertoire of these salivary proteins will give us insights into the molecular evolution of these proteins and will help us select relevant antigens for the development of a vector based anti-Leishmania vaccine. Results: Two salivary gland cDNA libraries from female sandflies Phlebotomus argentipes and P. perniciosus were constructed, sequenced and proteomic analysis of the salivary proteins was performed. The majority of the sequenced transcripts from the two cDNA libraries coded for secreted proteins. In this analysis we identified transcripts coding for protein families not previously described in sandflies. A comparative sandfly salivary transcriptome analysis was performed by using these two cDNA libraries and two other sandfly salivary gland cDNA libraries from P. ariasi and Lutzomyia longipalpis, also vectors of visceral leishmaniasis. Full-length secreted proteins from each sandfly library were compared using a stand-alone version of BLAST, creating formatted protein databases of each sandfly library. Related groups of proteins from each sandfly species were combined into defined families of proteins. With this comparison, we identified families of salivary proteins common among all of the sandflies studied, proteins to be genus specific and proteins that appear to be species specific. The common proteins included apyrase, yellow-related protein, antigen-5, PpSP15 and PpSP32-related protein, a 33-kDa protein, D7-related protein, a 39- and a 16.1- kDa protein and an endonuclease-like protein. Some of these families contained multiple members, including PPSP15-like, yellow proteins and D7-related proteins suggesting gene expansion in these proteins. Conclusion: This comprehensive analysis allows us the identification of genus- specific proteins, species-specific proteins and, more importantly, proteins common among these different sandflies. These results give us insights into the repertoire of salivary proteins that are potential candidates for a vector-based vaccine.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-08-28T14:08:47Z No. of bitstreams: 1 1471-2164-7-52.pdf: 1278771 bytes, checksum: bce26a4c87a4bb79671064aad19cb512 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-31T17:44:53Z (GMT) No. of bitstreams: 1 1471-2164-7-52.pdf: 1278771 bytes, checksum: bce26a4c87a4bb79671064aad19cb512 (MD5)Made available in DSpace on 2013-10-31T17:44:53Z (GMT). No. of bitstreams: 1 1471-2164-7-52.pdf: 1278771 bytes, checksum: bce26a4c87a4bb79671064aad19cb512 (MD5) Previous issue date: 2006http://dx.doi.org/ 10.1186/1471-2164-7-52reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAComparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasisBMC Genomicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL1471-2164-7-52.pdf1471-2164-7-52.pdfapplication/pdf1278771https://repositorio.ufba.br/bitstream/ri/13405/1/1471-2164-7-52.pdfbce26a4c87a4bb79671064aad19cb512MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13405/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1471-2164-7-52.pdf.txt1471-2164-7-52.pdf.txtExtracted texttext/plain109952https://repositorio.ufba.br/bitstream/ri/13405/3/1471-2164-7-52.pdf.txt488b0e740c82cba2edeb7b096a61f850MD53ri/134052022-07-05 14:02:55.415oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:55Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
dc.title.alternative.pt_BR.fl_str_mv BMC Genomics
title Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
spellingShingle Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
Oliveira, Fabiano
title_short Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
title_full Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
title_fullStr Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
title_full_unstemmed Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
title_sort Comparative salivary gland transcriptomics of sandfly vectors of visceral leishmaniasis
author Oliveira, Fabiano
author_facet Oliveira, Fabiano
Anderson, Jennifer M.
Kamhawi, Shaden
Mans, Ben J.
Reynoso, David
Seitz, Amy E.
Lawyer, Phillip
Garfield, Mark
Pham, MyVan
Valenzuela, Jesus G.
author_role author
author2 Anderson, Jennifer M.
Kamhawi, Shaden
Mans, Ben J.
Reynoso, David
Seitz, Amy E.
Lawyer, Phillip
Garfield, Mark
Pham, MyVan
Valenzuela, Jesus G.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Fabiano
Anderson, Jennifer M.
Kamhawi, Shaden
Mans, Ben J.
Reynoso, David
Seitz, Amy E.
Lawyer, Phillip
Garfield, Mark
Pham, MyVan
Valenzuela, Jesus G.
Oliveira, Fabiano
Anderson, Jennifer M.
Kamhawi, Shaden
Mans, Ben J.
Reynoso, David
Seitz, Amy E.
Lawyer, Phillip
Garfield, Mark
Pham, MyVan
Valenzuela, Jesus G.
description p.1-23
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2013-10-31T17:44:53Z
dc.date.available.fl_str_mv 2013-10-31T17:44:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13405
dc.identifier.issn.none.fl_str_mv 1471-2164
dc.identifier.number.pt_BR.fl_str_mv v.7 n. 52
identifier_str_mv 1471-2164
v.7 n. 52
url http://repositorio.ufba.br/ri/handle/ri/13405
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1186/1471-2164-7-52
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/13405/1/1471-2164-7-52.pdf
https://repositorio.ufba.br/bitstream/ri/13405/2/license.txt
https://repositorio.ufba.br/bitstream/ri/13405/3/1471-2164-7-52.pdf.txt
bitstream.checksum.fl_str_mv bce26a4c87a4bb79671064aad19cb512
1b89a9a0548218172d7c829f87a0eab9
488b0e740c82cba2edeb7b096a61f850
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1801502515165921280

Registros relacionados