Phenotype characterization of pericytes during tissue repair following low-level laser therapy

Detalhes bibliográficos
Autor(a) principal: Medrado, Alena Ribeiro Alves Peixoto
Data de Publicação: 2010
Outros Autores: Costa, Tila, Prado, Thiago, Reis, Sílvia, Andrade, Zilton de Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5678
Resumo: Acesso restrito: Texto completo. p. 192-197
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spelling Medrado, Alena Ribeiro Alves PeixotoCosta, TilaPrado, ThiagoReis, SílviaAndrade, Zilton de AraújoMedrado, Alena Ribeiro Alves PeixotoCosta, TilaPrado, ThiagoReis, SílviaAndrade, Zilton de Araújo2012-04-20T11:28:58Z20100905-4383http://www.repositorio.ufba.br/ri/handle/ri/5678v. 26, n. 4Acesso restrito: Texto completo. p. 192-197Background/purpose: The action of low-level laser therapy (LLLT) on pericytes during wound healing is not well established. The objective of this study was to identify the effect of laser treatment on pericytes during tissue repair. Methods: Punch biopsies were performed on 40 Wistar rats. Twenty animals had their wounds treated with a dose of 4 J/cm2 using a 670 nm diode laser (9 mW output, 0.031 W/cm2) every other day, while the controls received sham irradiation. Animals were sacrificed 3, 7, 10 and 14 days after punch biopsy. Immunohistochemistry staining with anti-desmin, anti-smooth muscle α-actin and anti-NG2 antibodies was used to characterize and count pericytes around blood vessels and myofibroblasts dispersed in the extracellular matrix (ECM). The morphology of pericytes was confirmed by transmission electronic microscopy. Results: The laser group exhibited significantly more smooth muscle α-actin-positive staining cells at day 7 and more desmin-positive staining cells at day 10 around blood vessels. Laser treatment was also associated with higher numbers of NG2-positive staining cells, especially on days 3 and 7 post-biopsy (P<0.05). Ultrastructural findings confirmed the presence of pericytes sharing the basal membrane with endothelial cells. Conclusion: LLLT stimulated the proliferation and migration of pericytes to the ECM and their phenotypic modulation to myofibroblasts.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-04-20T11:28:58Z No. of bitstreams: 1 __onlinelibrary.wiley.co...586d443196f4bce44624c35362e2b.pdf: 193061 bytes, checksum: 6f737f09c4d46d65638735ab4481e4f0 (MD5)Made available in DSpace on 2012-04-20T11:28:58Z (GMT). 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dc.title.pt_BR.fl_str_mv Phenotype characterization of pericytes during tissue repair following low-level laser therapy
dc.title.alternative.pt_BR.fl_str_mv Photodermatology Photoimmunology & Photomedicine
title Phenotype characterization of pericytes during tissue repair following low-level laser therapy
spellingShingle Phenotype characterization of pericytes during tissue repair following low-level laser therapy
Medrado, Alena Ribeiro Alves Peixoto
Extracellular matrix
Fhotobiostimulation
Wound repair
title_short Phenotype characterization of pericytes during tissue repair following low-level laser therapy
title_full Phenotype characterization of pericytes during tissue repair following low-level laser therapy
title_fullStr Phenotype characterization of pericytes during tissue repair following low-level laser therapy
title_full_unstemmed Phenotype characterization of pericytes during tissue repair following low-level laser therapy
title_sort Phenotype characterization of pericytes during tissue repair following low-level laser therapy
author Medrado, Alena Ribeiro Alves Peixoto
author_facet Medrado, Alena Ribeiro Alves Peixoto
Costa, Tila
Prado, Thiago
Reis, Sílvia
Andrade, Zilton de Araújo
author_role author
author2 Costa, Tila
Prado, Thiago
Reis, Sílvia
Andrade, Zilton de Araújo
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Medrado, Alena Ribeiro Alves Peixoto
Costa, Tila
Prado, Thiago
Reis, Sílvia
Andrade, Zilton de Araújo
Medrado, Alena Ribeiro Alves Peixoto
Costa, Tila
Prado, Thiago
Reis, Sílvia
Andrade, Zilton de Araújo
dc.subject.por.fl_str_mv Extracellular matrix
Fhotobiostimulation
Wound repair
topic Extracellular matrix
Fhotobiostimulation
Wound repair
description Acesso restrito: Texto completo. p. 192-197
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-04-20T11:28:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5678
dc.identifier.issn.none.fl_str_mv 0905-4383
dc.identifier.number.pt_BR.fl_str_mv v. 26, n. 4
identifier_str_mv 0905-4383
v. 26, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/5678
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv DOI: 10.1111/j.1600-0781.2010.00521.x
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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