An improved method to obtain antigen-excreting Toxocara canis larvae

Detalhes bibliográficos
Autor(a) principal: Alcântara-Neves, Neuza Maria
Data de Publicação: 2008
Outros Autores: Santos, Alex Barbosa dos, Mendonça, Lívia Ribeiro, Figueiredo, Camila A. V., Carvalho, Lain Carlos Pontes de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6117
Resumo: Acesso restrito: Texto completo. p. 349-351
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spelling Alcântara-Neves, Neuza MariaSantos, Alex Barbosa dosMendonça, Lívia RibeiroFigueiredo, Camila A. V.Carvalho, Lain Carlos Pontes deAlcântara-Neves, Neuza MariaSantos, Alex Barbosa dosMendonça, Lívia RibeiroFigueiredo, Camila A. V.Carvalho, Lain Carlos Pontes de2012-06-13T17:44:41Z20080014-4894http://www.repositorio.ufba.br/ri/handle/ri/6117v. 119, n. 3Acesso restrito: Texto completo. p. 349-351Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory–secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stools, the separation of eggs from dissected uteri, and the in vitro growing of egg-derived larvae, following the time-consuming and laborious protocol described by De Savigny [De Savigny, D.H., 1975. In vitro maintenance of T. canis larvae and a simple method for the production of Toxocara ES antigen for the uses in serodiagnostic tests for visceral larva migrans. Journal of Parasitology 61, 781–782]. In this work, an improved protocol for obtaining T. canis larvae is described. The modifications proposed improved the efficiency of the original De Savigny method in three ways: (i) increasing the parasite yield up to five fold, (ii) improving the larval purity, and (iii) markedly reducing the execution time of the protocol.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-06-13T17:44:41Z No. of bitstreams: 1 __ac.els-cdn.com_S001448...7b9d492db8ce9d4d4d5e6453e9e7d.pdf: 136487 bytes, checksum: b9c474178d45ca30a81eae62875dc46f (MD5)Made available in DSpace on 2012-06-13T17:44:41Z (GMT). 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dc.title.pt_BR.fl_str_mv An improved method to obtain antigen-excreting Toxocara canis larvae
dc.title.alternative.pt_BR.fl_str_mv Experimental Parasitology
title An improved method to obtain antigen-excreting Toxocara canis larvae
spellingShingle An improved method to obtain antigen-excreting Toxocara canis larvae
Alcântara-Neves, Neuza Maria
title_short An improved method to obtain antigen-excreting Toxocara canis larvae
title_full An improved method to obtain antigen-excreting Toxocara canis larvae
title_fullStr An improved method to obtain antigen-excreting Toxocara canis larvae
title_full_unstemmed An improved method to obtain antigen-excreting Toxocara canis larvae
title_sort An improved method to obtain antigen-excreting Toxocara canis larvae
author Alcântara-Neves, Neuza Maria
author_facet Alcântara-Neves, Neuza Maria
Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila A. V.
Carvalho, Lain Carlos Pontes de
author_role author
author2 Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila A. V.
Carvalho, Lain Carlos Pontes de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Alcântara-Neves, Neuza Maria
Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila A. V.
Carvalho, Lain Carlos Pontes de
Alcântara-Neves, Neuza Maria
Santos, Alex Barbosa dos
Mendonça, Lívia Ribeiro
Figueiredo, Camila A. V.
Carvalho, Lain Carlos Pontes de
description Acesso restrito: Texto completo. p. 349-351
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-06-13T17:44:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6117
dc.identifier.issn.none.fl_str_mv 0014-4894
dc.identifier.number.pt_BR.fl_str_mv v. 119, n. 3
identifier_str_mv 0014-4894
v. 119, n. 3
url http://www.repositorio.ufba.br/ri/handle/ri/6117
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.exppara.2008.03.006
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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