Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study

Detalhes bibliográficos
Autor(a) principal: Gois, Mariana M.
Data de Publicação: 2010
Outros Autores: Kurachi, Christina, Santana, E. J. B., Mima, E. G. O., Spolidório, D. M. P., Pelino, José Eduardo Pelizon, Bagnato, Vanderlei Salvador
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5809
Resumo: Acesso restrito: Texto completo. p. 391-395
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spelling Gois, Mariana M.Kurachi, ChristinaSantana, E. J. B.Mima, E. G. O.Spolidório, D. M. P.Pelino, José Eduardo PelizonBagnato, Vanderlei SalvadorGois, Mariana M.Kurachi, ChristinaSantana, E. J. B.Mima, E. G. O.Spolidório, D. M. P.Pelino, José Eduardo PelizonBagnato, Vanderlei Salvador2012-05-09T18:02:28Z20100268-8921http://www.repositorio.ufba.br/ri/handle/ri/5809v. 25, n. 3Acesso restrito: Texto completo. p. 391-395The ability of Staphylococcus aureus to develop multidrug resistance is well documented, and the antibiotic resistance showed by an increasing number of bacteria has shown the need for alternative therapies to treat infections, photodynamic therapy (PDT) being a potential candidate.The aim of this study was to determine the effect of photodynamic therapy as a light–based bactericidal modality to eliminate Staphylococcus aureus. The study investigated a technique based on a combination of light and a photosensitizer that is capable of producing oxidative species to induce a cytotoxic effect. A Staphylococcus aureus suspension was exposed to a light emitting diode (LED) emitting at 628 nm, 14.6 mW/cm2, and energy density of 20J/cm2, 40J/cm2, or 60 J/cm2 in the presence of different porphyrin concentrations (Photogem®). Three drug concentrations were employed: 12 μl/ml, 25 μl/ml, and 50 μl/ml.The treatment response was evaluated by the number of bacterial colony forming units (CFU) after light exposure.The results indicated that exposure to 60 J/cm2 eliminated 100% (10 log10 scales) of bacteria, on average. The best PDT response rate to eliminate Staphylococcus aureus was achieved with exposure to LED light in combination with the photosensitizer at concentrations ranging from 25 μl/ml to 50 μl/ml. These data suggest that PDT has the potential to eliminate Staphylococcus aureus in suspension and indicates the necessary drug concentration and light fluency.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-05-09T18:02:28Z No. of bitstreams: 1 __www.springerlink.com_c...40773340m5118130_fulltext.pdf: 121082 bytes, checksum: e0a4df8c17af683c4af14c13c3aefec9 (MD5)Made available in DSpace on 2012-05-09T18:02:28Z (GMT). No. of bitstreams: 1 __www.springerlink.com_c...40773340m5118130_fulltext.pdf: 121082 bytes, checksum: e0a4df8c17af683c4af14c13c3aefec9 (MD5) Previous issue date: 2010Springer VerlagDOI 10.1007/s10103-009-0705-0reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABacteriaBactericidal effectPhotodynamic therapySusceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro studyLASERS IN MEDICAL SCIENCEinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL__www.springerlink.com_c...40773340m5118130_fulltext.pdf__www.springerlink.com_c...40773340m5118130_fulltext.pdfapplication/pdf121082https://repositorio.ufba.br/bitstream/ri/5809/1/__www.springerlink.com_c...40773340m5118130_fulltext.pdfe0a4df8c17af683c4af14c13c3aefec9MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5809/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT__www.springerlink.com_c...40773340m5118130_fulltext.pdf.txt__www.springerlink.com_c...40773340m5118130_fulltext.pdf.txtExtracted texttext/plain22695https://repositorio.ufba.br/bitstream/ri/5809/3/__www.springerlink.com_c...40773340m5118130_fulltext.pdf.txt21c6b31cf4f8ea8493d1fed3a21673bfMD53ri/58092022-07-05 14:03:12.531oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:12Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
dc.title.alternative.pt_BR.fl_str_mv LASERS IN MEDICAL SCIENCE
title Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
spellingShingle Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
Gois, Mariana M.
Bacteria
Bactericidal effect
Photodynamic therapy
title_short Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
title_full Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
title_fullStr Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
title_full_unstemmed Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
title_sort Susceptibility of Staphylococcus aureus to porphyrin-mediated photodynamic antimicrobial chemotherapy: an in vitro study
author Gois, Mariana M.
author_facet Gois, Mariana M.
Kurachi, Christina
Santana, E. J. B.
Mima, E. G. O.
Spolidório, D. M. P.
Pelino, José Eduardo Pelizon
Bagnato, Vanderlei Salvador
author_role author
author2 Kurachi, Christina
Santana, E. J. B.
Mima, E. G. O.
Spolidório, D. M. P.
Pelino, José Eduardo Pelizon
Bagnato, Vanderlei Salvador
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gois, Mariana M.
Kurachi, Christina
Santana, E. J. B.
Mima, E. G. O.
Spolidório, D. M. P.
Pelino, José Eduardo Pelizon
Bagnato, Vanderlei Salvador
Gois, Mariana M.
Kurachi, Christina
Santana, E. J. B.
Mima, E. G. O.
Spolidório, D. M. P.
Pelino, José Eduardo Pelizon
Bagnato, Vanderlei Salvador
dc.subject.por.fl_str_mv Bacteria
Bactericidal effect
Photodynamic therapy
topic Bacteria
Bactericidal effect
Photodynamic therapy
description Acesso restrito: Texto completo. p. 391-395
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-05-09T18:02:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5809
dc.identifier.issn.none.fl_str_mv 0268-8921
dc.identifier.number.pt_BR.fl_str_mv v. 25, n. 3
identifier_str_mv 0268-8921
v. 25, n. 3
url http://www.repositorio.ufba.br/ri/handle/ri/5809
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.pt_BR.fl_str_mv DOI 10.1007/s10103-009-0705-0
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
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