Comparison of methods for mycobacteria isolation from swine feces

Detalhes bibliográficos
Autor(a) principal: Oliveira, Eugênia Márcia de Deus
Data de Publicação: 2007
Outros Autores: Rodriguez, César Alejandro Rosales, Rocha, Vivianne Cambuí Mesquita, Ambrosio, Simone Rodriguez, Ohara, Patrícia Miyuki, Amaku, Marcos, Ferreira, Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7015
Resumo: p. 687-692
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spelling Oliveira, Eugênia Márcia de DeusRodriguez, César Alejandro RosalesRocha, Vivianne Cambuí MesquitaAmbrosio, Simone RodriguezOhara, Patrícia MiyukiAmaku, MarcosFerreira, FernandoOliveira, Eugênia Márcia de DeusRodriguez, César Alejandro RosalesRocha, Vivianne Cambuí MesquitaAmbrosio, Simone RodriguezOhara, Patrícia MiyukiAmaku, MarcosFerreira, Fernando2012-10-22T14:13:25Z2012-10-22T14:13:25Z20071517-8382http://www.repositorio.ufba.br/ri/handle/ri/7015v. 38, n. 4p. 687-692Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery).Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-10-22T14:13:25Z No. of bitstreams: 1 __www.scielo.br_pdf_bjm_v38n4_a19v38n4.pdf: 146191 bytes, checksum: a0d3383dcb6c5264b8d49fc5337ef540 (MD5)Made available in DSpace on 2012-10-22T14:13:25Z (GMT). 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dc.title.pt_BR.fl_str_mv Comparison of methods for mycobacteria isolation from swine feces
dc.title.alternative.pt_BR.fl_str_mv Brazilian Journal of Microbiology
title Comparison of methods for mycobacteria isolation from swine feces
spellingShingle Comparison of methods for mycobacteria isolation from swine feces
Oliveira, Eugênia Márcia de Deus
Mycobacterium avium
isolation
feces
swine
title_short Comparison of methods for mycobacteria isolation from swine feces
title_full Comparison of methods for mycobacteria isolation from swine feces
title_fullStr Comparison of methods for mycobacteria isolation from swine feces
title_full_unstemmed Comparison of methods for mycobacteria isolation from swine feces
title_sort Comparison of methods for mycobacteria isolation from swine feces
author Oliveira, Eugênia Márcia de Deus
author_facet Oliveira, Eugênia Márcia de Deus
Rodriguez, César Alejandro Rosales
Rocha, Vivianne Cambuí Mesquita
Ambrosio, Simone Rodriguez
Ohara, Patrícia Miyuki
Amaku, Marcos
Ferreira, Fernando
author_role author
author2 Rodriguez, César Alejandro Rosales
Rocha, Vivianne Cambuí Mesquita
Ambrosio, Simone Rodriguez
Ohara, Patrícia Miyuki
Amaku, Marcos
Ferreira, Fernando
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Eugênia Márcia de Deus
Rodriguez, César Alejandro Rosales
Rocha, Vivianne Cambuí Mesquita
Ambrosio, Simone Rodriguez
Ohara, Patrícia Miyuki
Amaku, Marcos
Ferreira, Fernando
Oliveira, Eugênia Márcia de Deus
Rodriguez, César Alejandro Rosales
Rocha, Vivianne Cambuí Mesquita
Ambrosio, Simone Rodriguez
Ohara, Patrícia Miyuki
Amaku, Marcos
Ferreira, Fernando
dc.subject.por.fl_str_mv Mycobacterium avium
isolation
feces
swine
topic Mycobacterium avium
isolation
feces
swine
description p. 687-692
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2012-10-22T14:13:25Z
dc.date.available.fl_str_mv 2012-10-22T14:13:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7015
dc.identifier.issn.none.fl_str_mv 1517-8382
dc.identifier.number.pt_BR.fl_str_mv v. 38, n. 4
identifier_str_mv 1517-8382
v. 38, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/7015
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S1517-83822007000400019
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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