Comparison of methods for mycobacteria isolation from swine feces
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UNIFESP |
| Texto Completo: | http://dx.doi.org/10.1590/S1517-83822007000400019 http://repositorio.unifesp.br/handle/11600/4056 |
Resumo: | Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery). |
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Comparison of methods for mycobacteria isolation from swine fecesComparação de métodos para isolamento de micobactérias a partir de fezes suínasMycobacterium aviumisolationfecesswineMycobacterium aviumisolamentofezessuínosSwine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery).As micobacterioses suínas são responsáveis por condenações de carcaças em abatedouro e uma das melhores formas de se conhecer os agentes envolvidos nos animais vivos é o isolamento a partir das fezes, pois em 94% das vezes, as lesões localizam-se no trato digestivo. Assim sendo, o presente estudo teve por objetivo comparar o desempenho de quatro métodos de descontaminação com semeadura em três diferentes meios de cultura, totalizando doze procedimentos na pesquisa de micobactérias a partir de amostras de fezes de suínos contaminadas experimentalmente. Amostras de fezes de suínos foram contaminadas artificialmente com 0,02g de Mycobacterium avium, estirpe de PIG-B, e submetidas à tentativa de isolamento de micobactérias, utilizando-se os seguintes protocolos de descontaminação: 1) Petroff modificado ou método básico; 2) Lowenstein-Jensen modificado ou método ácido; 3) Petroff modificado ou método básico e ressuspensão com anfotericina B; 4) Lowenstein-Jensen modificado ou método ácido e ressuspensão com anfotericina B; com subseqüente semeadura em meios de Petragnani, Lowenstein-Jensen e Lowenstein-Jensen com antibióticos (Penicilina G e Ácido nalidíxico). Houve diferença entre os percentuais de recuperação de micobactérias a partir das fezes de suínos (p<0,05) e o método ácido com ressuspensão em solução de anfotericina B e semeadura em meio de Lowenstein-Jensen com antibióticos apresentou os melhores resultados (87% de recuperação de micobactérias).Universidade Federal da Bahia Escola de Medicina Veterinária Departamento de Medicina Veterinária PreventivaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de MicrobiologiaUNIFESP, EPM, Depto. de MicrobiologiaSciELOSociedade Brasileira de MicrobiologiaUniversidade Federal da Bahia Escola de Medicina Veterinária Departamento de Medicina Veterinária PreventivaUniversidade Federal de São Paulo (UNIFESP)Oliveira, Eugenia Márcia de DeusRodriguez, César Alejandro RosalesRocha, Vivianne Cambuí MesquitaAmbrosio, Simone RodriguezOhara, Patrícia MiyukiAmaku, MarcosFerreira, FernandoDias, Ricardo AugustoLeao, Sylvia Cardoso [UNIFESP]Ferreira Neto, José Soares2015-06-14T13:37:15Z2015-06-14T13:37:15Z2007-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion687-692application/pdfhttp://dx.doi.org/10.1590/S1517-83822007000400019Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 38, n. 4, p. 687-692, 2007.10.1590/S1517-83822007000400019S1517-83822007000400019.pdf1517-8382S1517-83822007000400019http://repositorio.unifesp.br/handle/11600/4056WOS:000252898500019engBrazilian Journal of Microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UNIFESPinstname:Universidade Federal de São Paulo (UNIFESP)instacron:UNIFESP2024-08-06T07:57:44Zoai:repositorio.unifesp.br/:11600/4056Repositório InstitucionalPUBhttp://www.repositorio.unifesp.br/oai/requestbiblioteca.csp@unifesp.bropendoar:34652024-08-06T07:57:44Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP)false |
| dc.title.none.fl_str_mv |
Comparison of methods for mycobacteria isolation from swine feces Comparação de métodos para isolamento de micobactérias a partir de fezes suínas |
| title |
Comparison of methods for mycobacteria isolation from swine feces |
| spellingShingle |
Comparison of methods for mycobacteria isolation from swine feces Oliveira, Eugenia Márcia de Deus Mycobacterium avium isolation feces swine Mycobacterium avium isolamento fezes suínos |
| title_short |
Comparison of methods for mycobacteria isolation from swine feces |
| title_full |
Comparison of methods for mycobacteria isolation from swine feces |
| title_fullStr |
Comparison of methods for mycobacteria isolation from swine feces |
| title_full_unstemmed |
Comparison of methods for mycobacteria isolation from swine feces |
| title_sort |
Comparison of methods for mycobacteria isolation from swine feces |
| author |
Oliveira, Eugenia Márcia de Deus |
| author_facet |
Oliveira, Eugenia Márcia de Deus Rodriguez, César Alejandro Rosales Rocha, Vivianne Cambuí Mesquita Ambrosio, Simone Rodriguez Ohara, Patrícia Miyuki Amaku, Marcos Ferreira, Fernando Dias, Ricardo Augusto Leao, Sylvia Cardoso [UNIFESP] Ferreira Neto, José Soares |
| author_role |
author |
| author2 |
Rodriguez, César Alejandro Rosales Rocha, Vivianne Cambuí Mesquita Ambrosio, Simone Rodriguez Ohara, Patrícia Miyuki Amaku, Marcos Ferreira, Fernando Dias, Ricardo Augusto Leao, Sylvia Cardoso [UNIFESP] Ferreira Neto, José Soares |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade Federal da Bahia Escola de Medicina Veterinária Departamento de Medicina Veterinária Preventiva Universidade Federal de São Paulo (UNIFESP) |
| dc.contributor.author.fl_str_mv |
Oliveira, Eugenia Márcia de Deus Rodriguez, César Alejandro Rosales Rocha, Vivianne Cambuí Mesquita Ambrosio, Simone Rodriguez Ohara, Patrícia Miyuki Amaku, Marcos Ferreira, Fernando Dias, Ricardo Augusto Leao, Sylvia Cardoso [UNIFESP] Ferreira Neto, José Soares |
| dc.subject.por.fl_str_mv |
Mycobacterium avium isolation feces swine Mycobacterium avium isolamento fezes suínos |
| topic |
Mycobacterium avium isolation feces swine Mycobacterium avium isolamento fezes suínos |
| description |
Swine mycobacteriosis is an important cause of carcass condemnation at abattoirs. One of the best ways to recognize the etiologic agent involved, in live animals, is the fecal isolation, as 94% of the lesions are located in the digestive tract. Therefore, the goal of the present study was to compare the performance of four decontamination methods followed by inoculation in three different culture media, totalizing twelve procedures of mycobacteria search from swine fecal samples experimentally contaminated. The swine feces were artificially contaminated with 0.02 g of Mycobacterium avium, PIG-B strain, and subjected to mycobacteria isolation trial. The protocols used were: 1) modified Petroff or basic method; 2) modified Lowenstein-Jensen or acidic method; 3) modified Petroff or basic method with re-suspension in Amphotericin B; 4) modified Lowenstein-Jensen or acid method with re-suspension in Amphotericin B, followed by inoculation in Petragnani, Lowenstein-Jensen and Lowenstein-Jensen medium with antibiotics (Penicillin G and Nalidixic acid). There was a difference (p<0.05) between the mycobacterial recovery percentages from swine feces. The acid method with re-suspension in Amphotericin B solution and inoculation in Lowenstein-Jensen medium with antibiotics showed the best results (87% of mycobacteria recovery). |
| publishDate |
2007 |
| dc.date.none.fl_str_mv |
2007-12-01 2015-06-14T13:37:15Z 2015-06-14T13:37:15Z |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1517-83822007000400019 Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 38, n. 4, p. 687-692, 2007. 10.1590/S1517-83822007000400019 S1517-83822007000400019.pdf 1517-8382 S1517-83822007000400019 http://repositorio.unifesp.br/handle/11600/4056 WOS:000252898500019 |
| url |
http://dx.doi.org/10.1590/S1517-83822007000400019 http://repositorio.unifesp.br/handle/11600/4056 |
| identifier_str_mv |
Brazilian Journal of Microbiology. Sociedade Brasileira de Microbiologia, v. 38, n. 4, p. 687-692, 2007. 10.1590/S1517-83822007000400019 S1517-83822007000400019.pdf 1517-8382 S1517-83822007000400019 WOS:000252898500019 |
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eng |
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eng |
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Brazilian Journal of Microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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687-692 application/pdf |
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Sociedade Brasileira de Microbiologia |
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Sociedade Brasileira de Microbiologia |
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reponame:Repositório Institucional da UNIFESP instname:Universidade Federal de São Paulo (UNIFESP) instacron:UNIFESP |
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Universidade Federal de São Paulo (UNIFESP) |
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UNIFESP |
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UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP |
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Repositório Institucional da UNIFESP - Universidade Federal de São Paulo (UNIFESP) |
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biblioteca.csp@unifesp.br |
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1814268307382992896 |