dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13359 |
Resumo: | Texto completo: acesso restrito. p.1746–1752 |
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Almeida, Luiz Alberto Luz deNeff, Helmut FranzBeeby, T.Lima, A. M. N.Borre, M.Thirstrup, C.Zong, W.Almeida, Luiz Alberto Luz deNeff, Helmut FranzBeeby, T.Lima, A. M. N.Borre, M.Thirstrup, C.Zong, W.2013-10-30T18:08:28Z20050956-5663http://repositorio.ufba.br/ri/handle/ri/13359v.21 n. 9Texto completo: acesso restrito. p.1746–1752The suitability of high resolution, in situ dc-sheet resistance monitoring (SRM) as a simplified and reliable sensing technique towards detection and tracking of protein immobilization has been explored. Non-specific adsorption of bovine serum albumin (BSA) onto a very thin gold film, acting as the sensing resistor, has been employed as a model system. For comparison, the novel sensing method was combined with surface plasmon resonance (SPR) spectroscopy, using the same flow cell and sensing surface. Two different, well known adsorption states, involving a composite layer of irreversibly and reversibly bound BSA, were clearly resolved by both methods. Clearly structured, pronounced and fully reproducible film resistance modulations have been resolved in the associated SRM data. The transition from reversibly bound BSA to the diluted protein phase is associated with an unusually large decrease in the dc-sheet resistance. The observed resistance modulation magnitude for an adsorbed BSA monolayer corresponds to approximately 1%, and up to 100 mΩ at a 10 Ω sensing resistor. The sheet resistance of irreversibly bound BSA was determined to 0.24 kΩ/cm2, and the associated specific resistivity estimated to 1–2 × 104 Ω cm.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-08-28T14:18:29Z No. of bitstreams: 1 1-s2.0-S095656630500254X-main.pdf: 254632 bytes, checksum: 8f179c52cfde1dc7241a3e4835bfccdc (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-30T18:08:28Z (GMT) No. of bitstreams: 1 1-s2.0-S095656630500254X-main.pdf: 254632 bytes, checksum: 8f179c52cfde1dc7241a3e4835bfccdc (MD5)Made available in DSpace on 2013-10-30T18:08:28Z (GMT). No. of bitstreams: 1 1-s2.0-S095656630500254X-main.pdf: 254632 bytes, checksum: 8f179c52cfde1dc7241a3e4835bfccdc (MD5) Previous issue date: 2006http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.bios.2005.08.009reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBASheet resistanceBiosensorThin film detectionProtein adsorptionImmobilizationHopping conductiondc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal filmsBiosensors and Bioelectronicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S095656630500254X-main.pdf1-s2.0-S095656630500254X-main.pdfapplication/pdf254632https://repositorio.ufba.br/bitstream/ri/13359/1/1-s2.0-S095656630500254X-main.pdf8f179c52cfde1dc7241a3e4835bfccdcMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13359/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S095656630500254X-main.pdf.txt1-s2.0-S095656630500254X-main.pdf.txtExtracted texttext/plain34679https://repositorio.ufba.br/bitstream/ri/13359/3/1-s2.0-S095656630500254X-main.pdf.txt161a87efa56ba1f255ba29d820caf6a5MD53ri/133592022-09-15 11:36:27.201oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-09-15T14:36:27Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| dc.title.alternative.pt_BR.fl_str_mv |
Biosensors and Bioelectronics |
| title |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| spellingShingle |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films Almeida, Luiz Alberto Luz de Sheet resistance Biosensor Thin film detection Protein adsorption Immobilization Hopping conduction |
| title_short |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| title_full |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| title_fullStr |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| title_full_unstemmed |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| title_sort |
dc-Sheet resistance as sensitive monitoring tool of protein immobilization on thin metal films |
| author |
Almeida, Luiz Alberto Luz de |
| author_facet |
Almeida, Luiz Alberto Luz de Neff, Helmut Franz Beeby, T. Lima, A. M. N. Borre, M. Thirstrup, C. Zong, W. |
| author_role |
author |
| author2 |
Neff, Helmut Franz Beeby, T. Lima, A. M. N. Borre, M. Thirstrup, C. Zong, W. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Almeida, Luiz Alberto Luz de Neff, Helmut Franz Beeby, T. Lima, A. M. N. Borre, M. Thirstrup, C. Zong, W. Almeida, Luiz Alberto Luz de Neff, Helmut Franz Beeby, T. Lima, A. M. N. Borre, M. Thirstrup, C. Zong, W. |
| dc.subject.por.fl_str_mv |
Sheet resistance Biosensor Thin film detection Protein adsorption Immobilization Hopping conduction |
| topic |
Sheet resistance Biosensor Thin film detection Protein adsorption Immobilization Hopping conduction |
| description |
Texto completo: acesso restrito. p.1746–1752 |
| publishDate |
2005 |
| dc.date.issued.fl_str_mv |
2005 |
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2013-10-30T18:08:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://repositorio.ufba.br/ri/handle/ri/13359 |
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0956-5663 |
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v.21 n. 9 |
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0956-5663 v.21 n. 9 |
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http://repositorio.ufba.br/ri/handle/ri/13359 |
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