Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression

Detalhes bibliográficos
Autor(a) principal: Batista, Wagner L.
Data de Publicação: 2007
Outros Autores: Barros, Tânia Fraga, Goldman, Gustavo H., Morais, Flávia V., Puccia, Rosana
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14596
Resumo: Texto completo: acesso restrito. p. 347-356
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spelling Batista, Wagner L.Barros, Tânia FragaGoldman, Gustavo H.Morais, Flávia V.Puccia, RosanaBatista, Wagner L.Barros, Tânia FragaGoldman, Gustavo H.Morais, Flávia V.Puccia, Rosana2014-02-13T17:30:57Z20071087-1845http://repositorio.ufba.br/ri/handle/ri/14596v. 44, n. 5Texto completo: acesso restrito. p. 347-356The MDJ1/LON locus is conserved among pathogenic dimorphic fungi. We have mapped using DNase I footprinting and mobility shift assays three putative heat shock elements and one AP-1 binding domain (ARE) in the 5′ intergenic region shared by PbMDJ1and PbLON (ML) from Paracoccidioides brasiliensis. The region bearing an ARE-like towards PbLON also has an opposite skn-1-like element. We studied genetically and pathogenically distinct isolates Pb18 and Pb3, where ML is polymorphic and the number of elements detected was higher. The functionality of the elements was suggested by the stimulatory response of both genes to heat shock and oxidative stress. Co-regulation occurred upon heat shock from 36 to 42 °C and, only in Pb3, also during mycelium to yeast transformation (26–36 °C). In Pb18, PbMDJ1 seemed to be preferentially expressed in yeast. Our study might help understand regulation of genes involved in fungal adaptation to the host.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-28T16:21:24Z No. of bitstreams: 1 1-s2.0-S1087184506002076-main.pdf: 572302 bytes, checksum: c36965fdb01e708bb246dcf37b8e8fff (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-02-13T17:30:57Z (GMT) No. of bitstreams: 1 1-s2.0-S1087184506002076-main.pdf: 572302 bytes, checksum: c36965fdb01e708bb246dcf37b8e8fff (MD5)Made available in DSpace on 2014-02-13T17:30:57Z (GMT). No. of bitstreams: 1 1-s2.0-S1087184506002076-main.pdf: 572302 bytes, checksum: c36965fdb01e708bb246dcf37b8e8fff (MD5) Previous issue date: 2007http://dx.doi.org/10.1016/j.fgb.2006.11.002reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAParacoccidioides brasiliensisMDJ1LONTranscription elementsGene expressionHeat shockOxidative stressIdentification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expressionFungal Genetics and Biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessporORIGINAL1-s2.0-S1087184506002076-main.pdf1-s2.0-S1087184506002076-main.pdfapplication/pdf572302https://repositorio.ufba.br/bitstream/ri/14596/1/1-s2.0-S1087184506002076-main.pdfc36965fdb01e708bb246dcf37b8e8fffMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14596/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT1-s2.0-S1087184506002076-main.pdf.txt1-s2.0-S1087184506002076-main.pdf.txtExtracted texttext/plain48413https://repositorio.ufba.br/bitstream/ri/14596/3/1-s2.0-S1087184506002076-main.pdf.txt2b049b10a58d642f187b9953d98286e1MD53ri/145962022-08-08 13:17:37.373oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:37Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
dc.title.alternative.pt_BR.fl_str_mv Fungal Genetics and Biology
title Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
spellingShingle Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
Batista, Wagner L.
Paracoccidioides brasiliensis
MDJ1
LON
Transcription elements
Gene expression
Heat shock
Oxidative stress
title_short Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
title_full Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
title_fullStr Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
title_full_unstemmed Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
title_sort Identification of transcription elements in the 5′ intergenic region shared by LON and MDJ1 heat shock genes from the human pathogen Paracoccidioides brasiliensis. Evaluation of gene expression
author Batista, Wagner L.
author_facet Batista, Wagner L.
Barros, Tânia Fraga
Goldman, Gustavo H.
Morais, Flávia V.
Puccia, Rosana
author_role author
author2 Barros, Tânia Fraga
Goldman, Gustavo H.
Morais, Flávia V.
Puccia, Rosana
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Batista, Wagner L.
Barros, Tânia Fraga
Goldman, Gustavo H.
Morais, Flávia V.
Puccia, Rosana
Batista, Wagner L.
Barros, Tânia Fraga
Goldman, Gustavo H.
Morais, Flávia V.
Puccia, Rosana
dc.subject.por.fl_str_mv Paracoccidioides brasiliensis
MDJ1
LON
Transcription elements
Gene expression
Heat shock
Oxidative stress
topic Paracoccidioides brasiliensis
MDJ1
LON
Transcription elements
Gene expression
Heat shock
Oxidative stress
description Texto completo: acesso restrito. p. 347-356
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-02-13T17:30:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14596
dc.identifier.issn.none.fl_str_mv 1087-1845
dc.identifier.number.pt_BR.fl_str_mv v. 44, n. 5
identifier_str_mv 1087-1845
v. 44, n. 5
url http://repositorio.ufba.br/ri/handle/ri/14596
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.fgb.2006.11.002
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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