Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography

Detalhes bibliográficos
Autor(a) principal: Bispo, Márcia Sousa
Data de Publicação: 2002
Outros Autores: Oliveira, Rodolfo F. S. de, Reis, José Oscar N., Andrade, Jailson Bittencourt de, Veloso, Márcia Cristina da Cunha, Pinheiro, Heloísa Lúcia C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14493
Resumo: Texto completo: acesso restrito. p. 45-48
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spelling Bispo, Márcia SousaOliveira, Rodolfo F. S. deReis, José Oscar N.Andrade, Jailson Bittencourt deVeloso, Márcia Cristina da CunhaPinheiro, Heloísa Lúcia C.Bispo, Márcia SousaOliveira, Rodolfo F. S. deReis, José Oscar N.Andrade, Jailson Bittencourt deVeloso, Márcia Cristina da CunhaPinheiro, Heloísa Lúcia C.2014-01-28T16:03:32Z20020021-9665http://repositorio.ufba.br/ri/handle/ri/14493v. 40, n. 1Texto completo: acesso restrito. p. 45-48This work relates the development of an analytical methodology to simultaneously determine three methylxanthines (caffeine, theobromine, and theophylline) in beverages and urine samples based on reversed-phase high-performance liquid chromatography. Separation is made with a Bondesil C18 column using methanol-water-acetic acid or ethanol-water-acetic acid (20:75:5, v/v/v) as the mobile phase at 0.7 mL/min. Identification is made by absorbance detection at 273 nm. Under optimized conditions, the detection limit of the HPLC method is 0.1 pg/mL for all three methylxanthines. This method is applied to urine and to 25 different beverage samples, which included coffee, tea, chocolate, and coconut water. The concentration ranges determined in the beverages and urine are: < 0.1 pg/mL to 350 µg/mL and 3.21 µg/mL to 71.2 µg/mL for caffeine; < 0.1pg/mL to 32 µg mL and < 0.1 pg/mL to 13.2 µg/mL for theobromine; < 0.1 pg/mL to 47 µg/mL and < 0.1 pg/mL to 66.3 µg/mL for theophylline. The method proposed in this study is rapid and suitable for the simultaneous quantitation of methylxanthines in beverages and human urine samples and requires no extraction step or derivatization.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-01-28T16:03:32Z No. of bitstreams: 1 Marcia S. Bispo.pdf: 148925 bytes, checksum: 072542462e4ae0153637b6c746117d11 (MD5)Made available in DSpace on 2014-01-28T16:03:32Z (GMT). No. of bitstreams: 1 Marcia S. Bispo.pdf: 148925 bytes, checksum: 072542462e4ae0153637b6c746117d11 (MD5) Previous issue date: 2002http://dx.doi.org/ 10.1093/chromsci/40.1.45reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBASimultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid ChromatographyJournal of Chromatographic Scienceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALMarcia S. Bispo.pdfMarcia S. Bispo.pdfapplication/pdf148925https://repositorio.ufba.br/bitstream/ri/14493/1/Marcia%20S.%20Bispo.pdf072542462e4ae0153637b6c746117d11MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14493/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTMarcia S. Bispo.pdf.txtMarcia S. 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dc.title.pt_BR.fl_str_mv Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
dc.title.alternative.pt_BR.fl_str_mv Journal of Chromatographic Science
title Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
spellingShingle Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
Bispo, Márcia Sousa
title_short Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
title_full Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
title_fullStr Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
title_full_unstemmed Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
title_sort Simultaneous Determination of Caffeine, Theobromine, and Theophylline by High-Performance Liquid Chromatography
author Bispo, Márcia Sousa
author_facet Bispo, Márcia Sousa
Oliveira, Rodolfo F. S. de
Reis, José Oscar N.
Andrade, Jailson Bittencourt de
Veloso, Márcia Cristina da Cunha
Pinheiro, Heloísa Lúcia C.
author_role author
author2 Oliveira, Rodolfo F. S. de
Reis, José Oscar N.
Andrade, Jailson Bittencourt de
Veloso, Márcia Cristina da Cunha
Pinheiro, Heloísa Lúcia C.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Bispo, Márcia Sousa
Oliveira, Rodolfo F. S. de
Reis, José Oscar N.
Andrade, Jailson Bittencourt de
Veloso, Márcia Cristina da Cunha
Pinheiro, Heloísa Lúcia C.
Bispo, Márcia Sousa
Oliveira, Rodolfo F. S. de
Reis, José Oscar N.
Andrade, Jailson Bittencourt de
Veloso, Márcia Cristina da Cunha
Pinheiro, Heloísa Lúcia C.
description Texto completo: acesso restrito. p. 45-48
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2014-01-28T16:03:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14493
dc.identifier.issn.none.fl_str_mv 0021-9665
dc.identifier.number.pt_BR.fl_str_mv v. 40, n. 1
identifier_str_mv 0021-9665
v. 40, n. 1
url http://repositorio.ufba.br/ri/handle/ri/14493
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1093/chromsci/40.1.45
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