A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum

Detalhes bibliográficos
Autor(a) principal: Marques, Lucas M.
Data de Publicação: 2013
Outros Autores: Amorim, Aline T., Martins, Hellen Braga, Rezende, Izadora Souza, Barbosa, Maysa Santos, Lobão, Tassia Neves, Campos, Guilherme B., Timenetsky, Jorge
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14758
Resumo: Texto completo: acesso restrito. p. 670–674
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spelling Marques, Lucas M.Amorim, Aline T.Martins, Hellen BragaRezende, Izadora SouzaBarbosa, Maysa SantosLobão, Tassia NevesCampos, Guilherme B.Timenetsky, JorgeMarques, Lucas M.Amorim, Aline T.Martins, Hellen BragaRezende, Izadora SouzaBarbosa, Maysa SantosLobão, Tassia NevesCampos, Guilherme B.Timenetsky, Jorge2014-03-18T14:37:41Z20130378-1135http://repositorio.ufba.br/ri/handle/ri/14758v. 167, n. 3-4Texto completo: acesso restrito. p. 670–674Ureaplasma diversum in veterinary studies is an undesirable microbe, which may cause infection in bulls and may result in seminal vesiculitis, balanopostitis, and alterations in spermatozoids, whereas in cows, it may cause placentitis, fetal alveolitis, abortion, and birth of weak calves. U. diversum is released through organic secretions, especially semen, preputial and vaginal mucus, conjunctival secretion, and milk. The aim of the present study was to develop a TaqMan probe, highly sensitive and specific quantitative PCR (qPCR) assay for the detection and quantification of U. diversum from genital swabs of bovines. Primers and probes specific to U. diversum 16S rRNA gene were designed. The specificity, detection limit, intra- and inter-assay variability of qPCR to detect this ureaplasma was compared with the results of the conventional PCR assay (cPCR). Swabs of vaginal mucus from 169 cows were tested. The qPCR assay detected as few as 10 copies of U. diversum and was 100-fold more sensitive than the cPCR. No cross-reactivity with other Mollicutes or eubacteria was observed. U. diversum was detected in 79 swabs (46.42%) by qPCR, while using cPCR it was detected in 42 (25%) samples. The difference in cPCR and qPCR ureaplasma detection between healthy and sick animals was not statistically significant. But the U. diversum load in samples from animals with genital disorders was higher than in healthy animals. The qPCR assay developed herein is highly sensitive and specific for the detection and quantification of U. diversum in vaginal bovine samples.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-03-18T14:37:41Z No. of bitstreams: 1 1-s2.0-S0378113513003945-main.pdf: 658692 bytes, checksum: 054544deac50918909725a93f1af6e08 (MD5)Made available in DSpace on 2014-03-18T14:37:41Z (GMT). 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dc.title.pt_BR.fl_str_mv A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
dc.title.alternative.pt_BR.fl_str_mv Veterinary Microbiology
title A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
spellingShingle A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
Marques, Lucas M.
Ureaplasma diversum
qPCR
Bovines
title_short A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
title_full A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
title_fullStr A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
title_full_unstemmed A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
title_sort A quantitative TaqMan PCR assay for the detection of Ureaplasma diversum
author Marques, Lucas M.
author_facet Marques, Lucas M.
Amorim, Aline T.
Martins, Hellen Braga
Rezende, Izadora Souza
Barbosa, Maysa Santos
Lobão, Tassia Neves
Campos, Guilherme B.
Timenetsky, Jorge
author_role author
author2 Amorim, Aline T.
Martins, Hellen Braga
Rezende, Izadora Souza
Barbosa, Maysa Santos
Lobão, Tassia Neves
Campos, Guilherme B.
Timenetsky, Jorge
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Lucas M.
Amorim, Aline T.
Martins, Hellen Braga
Rezende, Izadora Souza
Barbosa, Maysa Santos
Lobão, Tassia Neves
Campos, Guilherme B.
Timenetsky, Jorge
Marques, Lucas M.
Amorim, Aline T.
Martins, Hellen Braga
Rezende, Izadora Souza
Barbosa, Maysa Santos
Lobão, Tassia Neves
Campos, Guilherme B.
Timenetsky, Jorge
dc.subject.por.fl_str_mv Ureaplasma diversum
qPCR
Bovines
topic Ureaplasma diversum
qPCR
Bovines
description Texto completo: acesso restrito. p. 670–674
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-03-18T14:37:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14758
dc.identifier.issn.none.fl_str_mv 0378-1135
dc.identifier.number.pt_BR.fl_str_mv v. 167, n. 3-4
identifier_str_mv 0378-1135
v. 167, n. 3-4
url http://repositorio.ufba.br/ri/handle/ri/14758
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetmic.2013.07.031
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