Altered phenotype and function of dendritic cells in individuals with chronic periodontitis

Detalhes bibliográficos
Autor(a) principal: Cury, Patricia Ramos
Data de Publicação: 2013
Outros Autores: Carmo, J. P., Horewicz, V. V., Santos, Jean Nunes dos, Barbuto, J. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14956
Resumo: Texto completo: acesso restrito. p. 1208–1216
id UFBA-2_37ff0f52c5988b40641fb4090e50a849
oai_identifier_str oai:repositorio.ufba.br:ri/14956
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Cury, Patricia RamosCarmo, J. P.Horewicz, V. V.Santos, Jean Nunes dosBarbuto, J. A.Cury, Patricia RamosCarmo, J. P.Horewicz, V. V.Santos, Jean Nunes dosBarbuto, J. A.2014-05-14T11:26:16Z20130003-9969http://repositorio.ufba.br/ri/handle/ri/14956v. 58, n. 9Texto completo: acesso restrito. p. 1208–1216Objective: To investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-MDDCs) derived from individuals with chronic periodontitis (CP) or healthy periodontal tissue (HP). Design: m-MDDCs derived from peripheral blood monocytes, cultured for 7 days in presence of interleukin (IL)-4 and granulocyte-macrophage colony stimulating factor (GM-CSF), were stimulated with lysates of Streptococcus sanguinis, Prevotella intermedia, Porphyromonas gingivalis, or Treponema denticola on day 4, and were then phenotyped. IL-10, IL-12 and IFN-gamma concentration in the supernatant of cultures were measured. Results: Expression of HLA-DR was lower in bacterial-unstimulated mature m-MDDC from CP compared to HP (p = 0.04), while expression of CD1a and CD123 were higher in CP. The expression pattern of HLA-DR, CD11c, CD123, and CD1a did not change on bacterial stimulation, regardless of the bacteria. Stimulation with P. intermedia upregulated CD80 and CD86 in CP cells (p ≤ 0.05). Production of IL-12p70 by bacterial-unstimulated m-MDDCs was 5.8-fold greater in CP compared to HP. Bacterial stimulation further increased IL-12p70 production while decreasing IL-10. Significantly more IFN-gamma was produced in co-cultures of CP m-MDDCs than with HP m-MDDCs when cells were stimulated with P. intermedia (p = 0.009). Conclusions: Bacterial-unstimulated m-MDDC from CP exhibited a more immature phenotype but a cytokine profile biased towards proinflammatory response; this pattern was maintained/exacerbated after bacterial stimulation. P. intermedia upregulated co-stimulatory molecules and IFN-gamma expression in CP m-MDDC. These events might contribute to periodontitis pathogenesis.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-05-12T12:13:37Z No. of bitstreams: 1 1-s2.0-S0003996913001076-main.pdf: 790125 bytes, checksum: 1d674670a8e87aa1c34b5fb7105a3784 (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-05-14T11:26:16Z (GMT) No. of bitstreams: 1 1-s2.0-S0003996913001076-main.pdf: 790125 bytes, checksum: 1d674670a8e87aa1c34b5fb7105a3784 (MD5)Made available in DSpace on 2014-05-14T11:26:16Z (GMT). No. of bitstreams: 1 1-s2.0-S0003996913001076-main.pdf: 790125 bytes, checksum: 1d674670a8e87aa1c34b5fb7105a3784 (MD5) Previous issue date: 2013http://dx.doi.org/10.1016/j.archoralbio.2013.03.013reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAImmune responsePeriodontal diseasePeriodontal bacteriaAltered phenotype and function of dendritic cells in individuals with chronic periodontitisArchives of Oral Biologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S0003996913001076-main.pdf1-s2.0-S0003996913001076-main.pdfapplication/pdf790125https://repositorio.ufba.br/bitstream/ri/14956/1/1-s2.0-S0003996913001076-main.pdf1d674670a8e87aa1c34b5fb7105a3784MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14956/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT1-s2.0-S0003996913001076-main.pdf.txt1-s2.0-S0003996913001076-main.pdf.txtExtracted texttext/plain31756https://repositorio.ufba.br/bitstream/ri/14956/3/1-s2.0-S0003996913001076-main.pdf.txt926bc46af4e7c6bea18015d19c048db2MD53ri/149562022-07-05 14:02:59.958oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:59Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
dc.title.alternative.pt_BR.fl_str_mv Archives of Oral Biology
title Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
spellingShingle Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
Cury, Patricia Ramos
Immune response
Periodontal disease
Periodontal bacteria
title_short Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
title_full Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
title_fullStr Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
title_full_unstemmed Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
title_sort Altered phenotype and function of dendritic cells in individuals with chronic periodontitis
author Cury, Patricia Ramos
author_facet Cury, Patricia Ramos
Carmo, J. P.
Horewicz, V. V.
Santos, Jean Nunes dos
Barbuto, J. A.
author_role author
author2 Carmo, J. P.
Horewicz, V. V.
Santos, Jean Nunes dos
Barbuto, J. A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cury, Patricia Ramos
Carmo, J. P.
Horewicz, V. V.
Santos, Jean Nunes dos
Barbuto, J. A.
Cury, Patricia Ramos
Carmo, J. P.
Horewicz, V. V.
Santos, Jean Nunes dos
Barbuto, J. A.
dc.subject.por.fl_str_mv Immune response
Periodontal disease
Periodontal bacteria
topic Immune response
Periodontal disease
Periodontal bacteria
description Texto completo: acesso restrito. p. 1208–1216
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-05-14T11:26:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14956
dc.identifier.issn.none.fl_str_mv 0003-9969
dc.identifier.number.pt_BR.fl_str_mv v. 58, n. 9
identifier_str_mv 0003-9969
v. 58, n. 9
url http://repositorio.ufba.br/ri/handle/ri/14956
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2013.03.013
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/14956/1/1-s2.0-S0003996913001076-main.pdf
https://repositorio.ufba.br/bitstream/ri/14956/2/license.txt
https://repositorio.ufba.br/bitstream/ri/14956/3/1-s2.0-S0003996913001076-main.pdf.txt
bitstream.checksum.fl_str_mv 1d674670a8e87aa1c34b5fb7105a3784
0d4b811ef71182510d2015daa7c8a900
926bc46af4e7c6bea18015d19c048db2
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459477835317248

Registros relacionados