A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5038 |
Resumo: | p.158–166 |
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Aguiar, Paulo Henrique PalisMelo, Stella Maria BarrouinFranke, Carlos RobertoSantos, Roberto Robson Borges dosSilva, Tânia Maria CorreiaMengel, José O.Santos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes deAguiar, Paulo Henrique PalisMelo, Stella Maria BarrouinFranke, Carlos RobertoSantos, Roberto Robson Borges dosSilva, Tânia Maria CorreiaMengel, José O.Santos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes de2012-01-06T11:19:44Z2012-01-06T11:19:44Z20071090-0233http://www.repositorio.ufba.br/ri/handle/ri/5038v. 137p.158–166This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of immune functions in dogs.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-06T11:19:44Z No. of bitstreams: 1 Aguiar PH A monoclonal antibody against....pdf: 959645 bytes, checksum: 6b7abb475f57d2b433e8582c23ace230 (MD5)Made available in DSpace on 2012-01-06T11:19:44Z (GMT). No. of bitstreams: 1 Aguiar PH A monoclonal antibody against....pdf: 959645 bytes, checksum: 6b7abb475f57d2b433e8582c23ace230 (MD5) Previous issue date: 2007CanineLeukocyteMonoclonal antibodiesCD45A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activityThe Veterinary Journalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALAguiar PH A monoclonal antibody against....pdfAguiar PH A monoclonal antibody against....pdfapplication/pdf959645https://repositorio.ufba.br/bitstream/ri/5038/1/Aguiar%20PH%20A%20monoclonal%20antibody%20against....pdf6b7abb475f57d2b433e8582c23ace230MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5038/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTAguiar PH A monoclonal antibody against....pdf.txtAguiar PH A monoclonal antibody against....pdf.txtExtracted texttext/plain32247https://repositorio.ufba.br/bitstream/ri/5038/3/Aguiar%20PH%20A%20monoclonal%20antibody%20against....pdf.txt9636c0b87a58fd620e5c0832ea852309MD53ri/50382022-08-08 13:17:25.318oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:25Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| dc.title.alternative.pt_BR.fl_str_mv |
The Veterinary Journal |
| title |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| spellingShingle |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity Aguiar, Paulo Henrique Palis Canine Leukocyte Monoclonal antibodies CD45 |
| title_short |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| title_full |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| title_fullStr |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| title_full_unstemmed |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| title_sort |
A monoclonal antibody against a canine CD45 homologue: Analysis of tissue distribution, biochemical properties and in vitro immunological activity |
| author |
Aguiar, Paulo Henrique Palis |
| author_facet |
Aguiar, Paulo Henrique Palis Melo, Stella Maria Barrouin Franke, Carlos Roberto Santos, Roberto Robson Borges dos Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author_role |
author |
| author2 |
Melo, Stella Maria Barrouin Franke, Carlos Roberto Santos, Roberto Robson Borges dos Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Aguiar, Paulo Henrique Palis Melo, Stella Maria Barrouin Franke, Carlos Roberto Santos, Roberto Robson Borges dos Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de Aguiar, Paulo Henrique Palis Melo, Stella Maria Barrouin Franke, Carlos Roberto Santos, Roberto Robson Borges dos Silva, Tânia Maria Correia Mengel, José O. Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| dc.subject.por.fl_str_mv |
Canine Leukocyte Monoclonal antibodies CD45 |
| topic |
Canine Leukocyte Monoclonal antibodies CD45 |
| description |
p.158–166 |
| publishDate |
2007 |
| dc.date.issued.fl_str_mv |
2007 |
| dc.date.accessioned.fl_str_mv |
2012-01-06T11:19:44Z |
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2012-01-06T11:19:44Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://www.repositorio.ufba.br/ri/handle/ri/5038 |
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1090-0233 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 137 |
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1090-0233 v. 137 |
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http://www.repositorio.ufba.br/ri/handle/ri/5038 |
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eng |
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