Monoclonal antibodies against an outer membrane protein from pathogenic Leptospira

• Autor(a) principal: Lüdtke,Charli Beatriz
• Data de Publicação: 2003
• Outros Autores: Coutinho,Mariana Loner, Jouglard,Sandra Denise Dorneles, Moreira,Cecília Nunes, Fernandes,Cláudia Hartleben Pinho, Brod,Claudiomar Soares, Haake,David A., Ko,Albert Icksang, Dellagostin,Odir Antonio, Aleixo,José Antonio Guimarães
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500001
• topic: leptospirosis; monoclonal antibodies; LipL32

Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT medium and screened in an indirect ELISA. One MAb produced was of the IgG2b isotype and the other was an IgM. MAbs specificity was confirmed by indirect ELISA and immunoblotting using purified rLipL32 and whole-cell antigen preparations from Escherichia coli (E. coli) expressing LipL32 and from pathogenic and non-pathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted with non-pathogenic Leptospira. The MAbs described have potential for use in diagnostic tests for leptospirosis.