Comparison of three decontamination methods for Mycobacterium bovis isolation

Detalhes bibliográficos
Autor(a) principal: Ambrosio, Simone Rodriguez
Data de Publicação: 2008
Outros Autores: Oliveira, Eugênia Márcia de Deus, Rosales Rodriguez, Cesar Alejandro, Ferreira Neto, José Soares, Amaku, Marcos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6692
Resumo: p.241-244
id UFBA-2_44867a2ee3adc10e5be4109382f0473f
oai_identifier_str oai:repositorio.ufba.br:ri/6692
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Ambrosio, Simone RodriguezOliveira, Eugênia Márcia de DeusRosales Rodriguez, Cesar AlejandroFerreira Neto, José SoaresAmaku, MarcosAmbrosio, Simone RodriguezOliveira, Eugênia Márcia de DeusRosales Rodriguez, Cesar AlejandroFerreira Neto, José SoaresAmaku, Marcos2012-09-04T19:15:21Z2012-09-04T19:15:21Z2008-041517-8382http://www.repositorio.ufba.br/ri/handle/ri/6692v. 39, n. 2p.241-244Sixty samples of tissue fragments with lesions suggestive of tuberculosis from bovine abattoirs, kept in saturated solution of sodium borate, were subjected to four treatments: 4% NaOH (Petroff Method), 12 % H2SO4 and 1.5% HPC (1-Hexadecylpyridinium Chloride) decontamination, and physiological saline solution (control). The HPC method showed the lowest contamination rate (3%) when compared to control (88%, p<0.001), NaOH (33%, p<0.001) and H2SO4 (21.7%, p<0.002). Regarding the isolation success, the HPC method was better (40%) than the control (3%, p<0.001), NaOH (13%, p=0.001) and H2SO4 (1.7%, p<0.001) methods. These results indicate that HPC is an alternative to the Petroff method.Submitted by Texeira Ana (atanateixeira@gmail.com) on 2012-09-04T19:15:21Z No. of bitstreams: 1 Ambrosio, Simone Rodrigues.pdf: 390973 bytes, checksum: 220ec9ecf6182f8e810fcf19f9865009 (MD5)Made available in DSpace on 2012-09-04T19:15:21Z (GMT). No. of bitstreams: 1 Ambrosio, Simone Rodrigues.pdf: 390973 bytes, checksum: 220ec9ecf6182f8e810fcf19f9865009 (MD5) Previous issue date: 2008-04São PauloSociedade Brasileira de Microbiologiahttp://dx.doi.org/10.1590/S1517-83822008000200008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAMycobacterium bovisDecontaminationPetroffHPCComparison of three decontamination methods for Mycobacterium bovis isolationBrazilian Journal of Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALAmbrosio, Simone Rodrigues.pdfAmbrosio, Simone Rodrigues.pdfapplication/pdf390973https://repositorio.ufba.br/bitstream/ri/6692/1/Ambrosio%2c%20Simone%20Rodrigues.pdf220ec9ecf6182f8e810fcf19f9865009MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6692/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTAmbrosio, Simone Rodrigues.pdf.txtAmbrosio, Simone Rodrigues.pdf.txtExtracted texttext/plain15879https://repositorio.ufba.br/bitstream/ri/6692/3/Ambrosio%2c%20Simone%20Rodrigues.pdf.txtf0e40499663e7cd77a37fb27c63773d0MD53ri/66922022-08-08 13:17:27.75oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:27Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Comparison of three decontamination methods for Mycobacterium bovis isolation
dc.title.alternative.pt_BR.fl_str_mv Brazilian Journal of Microbiology
title Comparison of three decontamination methods for Mycobacterium bovis isolation
spellingShingle Comparison of three decontamination methods for Mycobacterium bovis isolation
Ambrosio, Simone Rodriguez
Mycobacterium bovis
Decontamination
Petroff
HPC
title_short Comparison of three decontamination methods for Mycobacterium bovis isolation
title_full Comparison of three decontamination methods for Mycobacterium bovis isolation
title_fullStr Comparison of three decontamination methods for Mycobacterium bovis isolation
title_full_unstemmed Comparison of three decontamination methods for Mycobacterium bovis isolation
title_sort Comparison of three decontamination methods for Mycobacterium bovis isolation
author Ambrosio, Simone Rodriguez
author_facet Ambrosio, Simone Rodriguez
Oliveira, Eugênia Márcia de Deus
Rosales Rodriguez, Cesar Alejandro
Ferreira Neto, José Soares
Amaku, Marcos
author_role author
author2 Oliveira, Eugênia Márcia de Deus
Rosales Rodriguez, Cesar Alejandro
Ferreira Neto, José Soares
Amaku, Marcos
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Ambrosio, Simone Rodriguez
Oliveira, Eugênia Márcia de Deus
Rosales Rodriguez, Cesar Alejandro
Ferreira Neto, José Soares
Amaku, Marcos
Ambrosio, Simone Rodriguez
Oliveira, Eugênia Márcia de Deus
Rosales Rodriguez, Cesar Alejandro
Ferreira Neto, José Soares
Amaku, Marcos
dc.subject.por.fl_str_mv Mycobacterium bovis
Decontamination
Petroff
HPC
topic Mycobacterium bovis
Decontamination
Petroff
HPC
description p.241-244
publishDate 2008
dc.date.issued.fl_str_mv 2008-04
dc.date.accessioned.fl_str_mv 2012-09-04T19:15:21Z
dc.date.available.fl_str_mv 2012-09-04T19:15:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6692
dc.identifier.issn.none.fl_str_mv 1517-8382
dc.identifier.number.pt_BR.fl_str_mv v. 39, n. 2
identifier_str_mv 1517-8382
v. 39, n. 2
url http://www.repositorio.ufba.br/ri/handle/ri/6692
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S1517-83822008000200008
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/6692/1/Ambrosio%2c%20Simone%20Rodrigues.pdf
https://repositorio.ufba.br/bitstream/ri/6692/2/license.txt
https://repositorio.ufba.br/bitstream/ri/6692/3/Ambrosio%2c%20Simone%20Rodrigues.pdf.txt
bitstream.checksum.fl_str_mv 220ec9ecf6182f8e810fcf19f9865009
1b89a9a0548218172d7c829f87a0eab9
f0e40499663e7cd77a37fb27c63773d0
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459405710065664

Registros relacionados