Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells

Detalhes bibliográficos
Autor(a) principal: Freitas, E. S.
Data de Publicação: 2006
Outros Autores: Leite, E. D., Lima, Alessandra Estrela da Silva, Ocarino, Natália de Melo, Ferreira, E., Gomes, M. G., Cassali, Geovanni Dantas, Serakides, R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5352
Resumo: The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effect.
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spelling Freitas, E. S.Leite, E. D.Lima, Alessandra Estrela da SilvaOcarino, Natália de MeloFerreira, E.Gomes, M. G.Cassali, Geovanni DantasSerakides, R.Freitas, E. S.Leite, E. D.Lima, Alessandra Estrela da SilvaOcarino, Natália de MeloFerreira, E.Gomes, M. G.Cassali, Geovanni DantasSerakides, R.2012-02-08T22:04:04Z2012-02-08T22:04:04Z20060717-9502http://www.repositorio.ufba.br/ri/handle/ri/5352v. 24, n. 4The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effect.Submitted by Alessandra Estrela-Lima (aestrela@ufba.br) on 2012-02-08T22:04:04Z No. of bitstreams: 1 art24.pdf: 549109 bytes, checksum: 0942357e1126d82cc6868300d06fa802 (MD5)Made available in DSpace on 2012-02-08T22:04:04Z (GMT). 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dc.title.pt_BR.fl_str_mv Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
dc.title.alternative.pt_BR.fl_str_mv International Journal of Morphology
title Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
spellingShingle Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
Freitas, E. S.
Cytology
Ehrlich tumor
Mouse
NORs
Propylthiouracil
thyroxine
title_short Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
title_full Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
title_fullStr Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
title_full_unstemmed Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
title_sort Effect of thyroxine and propylthiouracil in Ehrlich ascitic tumor cells
author Freitas, E. S.
author_facet Freitas, E. S.
Leite, E. D.
Lima, Alessandra Estrela da Silva
Ocarino, Natália de Melo
Ferreira, E.
Gomes, M. G.
Cassali, Geovanni Dantas
Serakides, R.
author_role author
author2 Leite, E. D.
Lima, Alessandra Estrela da Silva
Ocarino, Natália de Melo
Ferreira, E.
Gomes, M. G.
Cassali, Geovanni Dantas
Serakides, R.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freitas, E. S.
Leite, E. D.
Lima, Alessandra Estrela da Silva
Ocarino, Natália de Melo
Ferreira, E.
Gomes, M. G.
Cassali, Geovanni Dantas
Serakides, R.
Freitas, E. S.
Leite, E. D.
Lima, Alessandra Estrela da Silva
Ocarino, Natália de Melo
Ferreira, E.
Gomes, M. G.
Cassali, Geovanni Dantas
Serakides, R.
dc.subject.por.fl_str_mv Cytology
Ehrlich tumor
Mouse
NORs
Propylthiouracil
thyroxine
topic Cytology
Ehrlich tumor
Mouse
NORs
Propylthiouracil
thyroxine
description The main purpose of this study was to investigate the effect of thyroxine and PTU in ascitic Ehrlich tumor cells. Tumor was implanted in 30 female mice distributed in three groups: treated with PTU, treated with thyroxine and control. Each group received an intraperitoneal injection of neoplastic cells, pre-incubated with sterile solutions of PTU, thyroxine and distilled water, respectively. On the fifth and seventh days after inoculation, animals received an intraperitoneal injection of the respective solutions. On the tenth day after inoculation, animals were sacrificed. Volume of ascitic liquid, number of neoplastic cells/ml and percentage of viable cells were determined. Ascitic liquid smears were carried out for tumor cytological evaluation. There was no difference among groups regarding ascitic liquid and as for the number and viability of tumor cells. However, cells under the effect of thyroxine presented significantly larger mean of nuclear diameter, size and number of nucleolus organizer regions. In this group, there was a predominance of clear, round cells with abundant eosinophilic and very vacuolated cytoplasm with little defined edges. Under the PTU effect, tumor cells were small with hyperchromatic nucleus and the same number of NORs as the control group. It was concluded that PTU and thyroxine have not changed the number and viability of cells after 10 days of tumor inoculation but they changed significantly cell characteristics. Whilst thyroxine increases cell size and the number of NORs of ascitic Ehrlich tumor cells, PTU causes an opposite effect.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2012-02-08T22:04:04Z
dc.date.available.fl_str_mv 2012-02-08T22:04:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5352
dc.identifier.issn.none.fl_str_mv 0717-9502
dc.identifier.number.pt_BR.fl_str_mv v. 24, n. 4
identifier_str_mv 0717-9502
v. 24, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/5352
dc.language.iso.fl_str_mv eng
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