Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion

Detalhes bibliográficos
Autor(a) principal: Silva, Tânia Regina Marques da
Data de Publicação: 2002
Outros Autores: Freitas, Juliana Ribeiro de, Silva, Queilan Chagas, Figueira, Cláudio Pereira, Roxo, Eliana, Leão, Sylvia Cardoso, Freitas, Luiz Antônio Rodrigues de, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7457
Resumo: Texto completo: acesso restrito. p. 5628-5634
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spelling Silva, Tânia Regina Marques daFreitas, Juliana Ribeiro deSilva, Queilan ChagasFigueira, Cláudio PereiraRoxo, ElianaLeão, Sylvia CardosoFreitas, Luiz Antônio Rodrigues deVeras, Patrícia Sampaio TavaresSilva, Tânia Regina Marques daFreitas, Juliana Ribeiro deSilva, Queilan ChagasFigueira, Cláudio PereiraRoxo, ElianaLeão, Sylvia CardosoFreitas, Luiz Antônio Rodrigues deVeras, Patrícia Sampaio Tavares2012-12-12T15:19:35Z2002-100019-9567http://www.repositorio.ufba.br/ri/handle/ri/7457v. 70, n. 10Texto completo: acesso restrito. p. 5628-5634The virulence of different isolates of Mycobacterium has been associated with two morphologically distinguishable colonial variants: opaque (SmOp) and transparent (SmTr). In this report we used an in vitro assay to compare macrophage (Mφ) responses to SmOp and SmTr Mycobacterium fortuitum variants, taking advantage of the fact that these variants were derived from the same isolate. Cells preactivated or not with gamma interferon (IFN-γ) were infected with SmOp or SmTr M. fortuitum. We showed that SmOp and SmTr induced different levels of nitric oxide (NO) production by IFN-γ-stimulated Mφ. Indeed, the amount of IFN-γ-induced NO production by J774 cells was 4.8 to 9.0 times higher by SmOp (23.1 to 37.7 μM) compared to SmTr infection (3.9 to 4.8 μM) (P = 0.0332), indicating that virulent SmTr bacilli restricted NO production. In addition, IFN-γ-induced NO production by Mφ was higher when correlated with reduction of only avirulent SmOp bacillus viability. SNAP (S-nitroso-N-acetyl-dl-penicillamine) -induced NO production did not modify SmTr viability, indicating its resistance to nitrogen radicals. Electron microscopy studies were performed to evaluate the capacity of phagosomes to fuse with lysosomes labeled with bovine serum albumin-colloidal gold particles. By 24 h postinfection, 69% more phagosome-containing SmOp variant had fused with lysosomes compared to the SmTr-induced phagosomes. In conclusion, these data indicate that virulent SmTr bacilli may escape host defense by restricting IFN-γ-induced NO production, resisting nitrogen toxic radicals, and limiting phagosome fusion with lysosomes.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-12T15:19:35Z No. of bitstreams: 1 5628.full.pdf: 1147506 bytes, checksum: 4052954928ce04e0ed9567c72d31dee5 (MD5)Made available in DSpace on 2012-12-12T15:19:35Z (GMT). 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dc.title.pt_BR.fl_str_mv Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
dc.title.alternative.pt_BR.fl_str_mv Infection and Immunity
title Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
spellingShingle Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
Silva, Tânia Regina Marques da
title_short Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
title_full Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
title_fullStr Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
title_full_unstemmed Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
title_sort Virulent Mycobacterium fortuitum restricts no production by a gamma interferon-activated j774 cell line and phagosome-lysosome fusion
author Silva, Tânia Regina Marques da
author_facet Silva, Tânia Regina Marques da
Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antônio Rodrigues de
Veras, Patrícia Sampaio Tavares
author_role author
author2 Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antônio Rodrigues de
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Tânia Regina Marques da
Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antônio Rodrigues de
Veras, Patrícia Sampaio Tavares
Silva, Tânia Regina Marques da
Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antônio Rodrigues de
Veras, Patrícia Sampaio Tavares
description Texto completo: acesso restrito. p. 5628-5634
publishDate 2002
dc.date.issued.fl_str_mv 2002-10
dc.date.accessioned.fl_str_mv 2012-12-12T15:19:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7457
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.number.pt_BR.fl_str_mv v. 70, n. 10
identifier_str_mv 0019-9567
v. 70, n. 10
url http://www.repositorio.ufba.br/ri/handle/ri/7457
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1128/IAI.70.10.5628-5634.2002
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