Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting

Paule, Bruno Jean Adrien; Azevedo, Vasco Ariston de Carvalho; Regis, Lia Fernandes; Bahia, Robson Cerqueira; Carminati, Renato; Vale, Vera Lucia Costa; Costa, Lilia Ferreira Moura; Freire, Songeli Menezes; Schaer, Robert Eduard; Nascimento, Ivana Lúcia de Oliveira; Goes, Alfredo Miranda de; Nascimento, Roberto José Meyer

Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting

Detalhes bibliográficos
Autor(a) principal:	Paule, Bruno Jean Adrien
Data de Publicação:	2003
Outros Autores:	Azevedo, Vasco Ariston de Carvalho, Regis, Lia Fernandes, Bahia, Robson Cerqueira, Carminati, Renato, Vale, Vera Lucia Costa, Costa, Lilia Ferreira Moura, Freire, Songeli Menezes, Schaer, Robert Eduard, Nascimento, Ivana Lúcia de Oliveira, Goes, Alfredo Miranda de, Nascimento, Roberto José Meyer
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da UFBA
Texto Completo:	http://www.repositorio.ufba.br/ri/handle/ri/5248
Resumo:	p.129–139

Metadados do item

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spelling	Paule, Bruno Jean AdrienAzevedo, Vasco Ariston de CarvalhoRegis, Lia FernandesBahia, Robson CerqueiraCarminati, RenatoVale, Vera Lucia CostaCosta, Lilia Ferreira MouraFreire, Songeli MenezesSchaer, Robert EduardNascimento, Ivana Lúcia de OliveiraGoes, Alfredo Miranda deNascimento, Roberto José MeyerPaule, Bruno Jean AdrienAzevedo, Vasco Ariston de CarvalhoRegis, Lia FernandesBahia, Robson CerqueiraCarminati, RenatoVale, Vera Lucia CostaCosta, Lilia Ferreira MouraFreire, Songeli MenezesSchaer, Robert EduardNascimento, Ivana Lúcia de OliveiraGoes, Alfredo Miranda deNascimento, Roberto José Meyer2012-01-25T16:28:45Z2012-01-25T16:28:45Z20030165-2427http://www.repositorio.ufba.br/ri/handle/ri/5248v. 96.p.129–139Corynebacterium pseudotuberculosis is the cause of caseous lymphadenitis (CLA) in small ruminants, a chronic granulomatous disease that provokes significant zootechnics losses to ovine and goat breeders in northern Brazil. The present work was conducted to analyse aspects of humoral and cellular immune responses after experimental infection. Eight goats were infected intradermally with a single dose of virulent C. pseudotuberculosis strain and specific IgG, interferon-g (IFN-g) production as well as IgG avidity and antigens pattern recognition dynamics against an excreted–secreted antigen were recorded during 20 weeks. At the end of the follow-up, animals were slaughtered and necropsied. Although no animals showed apparent clinical signs of infection at the end of the trial, IFN-g response, even more so than the humoral response, differentiated animals into two groups of high or medium/low response. The time-course of IFN-g production presented a short-lived primary response on day 5 after infection of animals of both groups, and a strong and long lasting secondary response starting on day 16 after infection in the high response group. The indirect ELISA used was able to detect a positive antibody titre between 6 and 11 days after infection in the two groups. IgG avidity index oscillated initially between 15 and 45%, and showed approximately 5% units increment during the 20 follow-up weeks. With only one individual exception, the qualitative antigens pattern recognition showed on day 11 after infection remained constant through the experiment. IgG avidity is highly correlated with IgG production, but could not be related with specific immunodominant bands. Both humoral and cellular responses kinetics presented a similar pattern of activation/deactivation but necropsy results suggested that the IFN-g test would be a very specific marker of CLA status.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-25T16:28:44Z No. of bitstreams: 1 S0165242703001466-main.pdf: 235186 bytes, checksum: 731a4741820d0d959992597c856df2fd (MD5)Made available in DSpace on 2012-01-25T16:28:45Z (GMT). No. of bitstreams: 1 S0165242703001466-main.pdf: 235186 bytes, checksum: 731a4741820d0d959992597c856df2fd (MD5) Previous issue date: 2003GoatsCorynebacterium pseudotuberculosisInterferon-gSerologyKineticCaseous lymphadenitisExperimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blottingVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALS0165242703001466-main.pdfS0165242703001466-main.pdfapplication/pdf235186https://repositorio.ufba.br/bitstream/ri/5248/1/S0165242703001466-main.pdf731a4741820d0d959992597c856df2fdMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5248/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTS0165242703001466-main.pdf.txtS0165242703001466-main.pdf.txtExtracted texttext/plain39544https://repositorio.ufba.br/bitstream/ri/5248/3/S0165242703001466-main.pdf.txtaa8bb1e7f7953918e0f20afc5287060cMD53ri/52482022-07-05 14:03:30.758oai:repositorio.ufba.br:ri/5248VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:30Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
dc.title.alternative.pt_BR.fl_str_mv	Veterinary Immunology and Immunopathology
title	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
spellingShingle	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting Paule, Bruno Jean Adrien Goats Corynebacterium pseudotuberculosis Interferon-g Serology Kinetic Caseous lymphadenitis
title_short	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
title_full	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
title_fullStr	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
title_full_unstemmed	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
title_sort	Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting
author	Paule, Bruno Jean Adrien
author_facet	Paule, Bruno Jean Adrien Azevedo, Vasco Ariston de Carvalho Regis, Lia Fernandes Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lucia Costa Costa, Lilia Ferreira Moura Freire, Songeli Menezes Schaer, Robert Eduard Nascimento, Ivana Lúcia de Oliveira Goes, Alfredo Miranda de Nascimento, Roberto José Meyer
author_role	author
author2	Azevedo, Vasco Ariston de Carvalho Regis, Lia Fernandes Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lucia Costa Costa, Lilia Ferreira Moura Freire, Songeli Menezes Schaer, Robert Eduard Nascimento, Ivana Lúcia de Oliveira Goes, Alfredo Miranda de Nascimento, Roberto José Meyer
author2_role	author author author author author author author author author author author
dc.contributor.author.fl_str_mv	Paule, Bruno Jean Adrien Azevedo, Vasco Ariston de Carvalho Regis, Lia Fernandes Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lucia Costa Costa, Lilia Ferreira Moura Freire, Songeli Menezes Schaer, Robert Eduard Nascimento, Ivana Lúcia de Oliveira Goes, Alfredo Miranda de Nascimento, Roberto José Meyer Paule, Bruno Jean Adrien Azevedo, Vasco Ariston de Carvalho Regis, Lia Fernandes Bahia, Robson Cerqueira Carminati, Renato Vale, Vera Lucia Costa Costa, Lilia Ferreira Moura Freire, Songeli Menezes Schaer, Robert Eduard Nascimento, Ivana Lúcia de Oliveira Goes, Alfredo Miranda de Nascimento, Roberto José Meyer
dc.subject.por.fl_str_mv	Goats Corynebacterium pseudotuberculosis Interferon-g Serology Kinetic Caseous lymphadenitis
topic	Goats Corynebacterium pseudotuberculosis Interferon-g Serology Kinetic Caseous lymphadenitis
description	p.129–139
publishDate	2003
dc.date.issued.fl_str_mv	2003
dc.date.accessioned.fl_str_mv	2012-01-25T16:28:45Z
dc.date.available.fl_str_mv	2012-01-25T16:28:45Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.uri.fl_str_mv	http://www.repositorio.ufba.br/ri/handle/ri/5248
dc.identifier.issn.none.fl_str_mv	0165-2427
dc.identifier.number.pt_BR.fl_str_mv	v. 96.
identifier_str_mv	0165-2427 v. 96.
url	http://www.repositorio.ufba.br/ri/handle/ri/5248
dc.language.iso.fl_str_mv	eng
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dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
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collection	Repositório Institucional da UFBA
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Experimental Corynebacterium pseudotuberculosis primary infection in goats: kinetics of IgG and interferon-g production, IgG avidity and antigen recognition by Western blotting

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