Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells

Detalhes bibliográficos
Autor(a) principal: Santos, B. L.
Data de Publicação: 2011
Outros Autores: Silva, A. R., Pitanga, B. P. S., Sousa, C. S., Grangeiro, M. S., Fragomeni, B. O., Coelho, P. L. C., Oliveira, M. N., Menezes Filho, N. J., Costa, Maria de Fátima Dias, El-Bachá, Ramon dos Santos, Velozo, Eudes da Silva, Sampaio, G. P., Freire, S. M., Tardy, Marcienne Bloch, Costa, Silvia Lima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14748
Resumo: Texto completo: acesso restrito. p. 404–411
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spelling Santos, B. L.Silva, A. R.Pitanga, B. P. S.Sousa, C. S.Grangeiro, M. S.Fragomeni, B. O.Coelho, P. L. C.Oliveira, M. N.Menezes Filho, N. J.Costa, Maria de Fátima DiasEl-Bachá, Ramon dos SantosVelozo, Eudes da SilvaSampaio, G. P.Freire, S. M.Tardy, Marcienne BlochCosta, Silvia LimaSantos, B. L.Silva, A. R.Pitanga, B. P. S.Sousa, C. S.Grangeiro, M. S.Fragomeni, B. O.Coelho, P. L. C.Oliveira, M. N.Menezes Filho, N. J.Costa, Maria de Fátima DiasEl-Bachá, Ramon dos SantosVelozo, Eudes da SilvaSampaio, G. P.Freire, S. M.Tardy, Marcienne BlochCosta, Silvia Lima2014-03-13T18:23:05Z20110308-8146http://repositorio.ufba.br/ri/handle/ri/14748v. 127, n. 2Texto completo: acesso restrito. p. 404–411In this study, we investigated the effects of the flavonoid rutin (3,3′,4′,5,7-pentahydroxyflavone-3-rutinoside) on glioma cells, using the highly proliferative human cell line GL-15 as a model. We observed that rutin (50–100 μM) reduced proliferation and viability of GL-15 cells, leading to decreased levels of ERK1/2 phosphorylation (P-ERK1/2) and accumulation of cells in the G2 phase of the cell cycle. On the other hand, 87.4% of GL-15 cells exposed to 100 μM rutin entered apoptosis, as revealed by flow cytometry after AnnexinV/PI staining. Nuclear condensation and DNA fragmentation were also observed, further confirming that apoptosis had occurred. Moreover, the remaining cells that were treated with 50 μM rutin presented a morphological pattern of astroglial differentiation in culture, characterised by a condensed cell body and thin processes with overexpression of GFAP. Because of its capacity to induce differentiation and apoptosis in cultured human glioblastoma cells, rutin could be considered as a potential candidate for malignant gliomas treatment.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-03-12T14:10:41Z No. of bitstreams: 1 B. L. Santos.pdf: 890946 bytes, checksum: 60964c86955f5c907304011c08596b7a (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-03-13T18:23:05Z (GMT) No. of bitstreams: 1 B. L. Santos.pdf: 890946 bytes, checksum: 60964c86955f5c907304011c08596b7a (MD5)Made available in DSpace on 2014-03-13T18:23:05Z (GMT). No. of bitstreams: 1 B. L. Santos.pdf: 890946 bytes, checksum: 60964c86955f5c907304011c08596b7a (MD5) Previous issue date: 2011http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.foodchem.2010.12.131reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBARutinFlavonoidGliomasApoptosisDifferentiationP-ERKGFAPAntiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cellsFood Chemistryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALB. L. Santos.pdfB. L. 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dc.title.pt_BR.fl_str_mv Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
dc.title.alternative.pt_BR.fl_str_mv Food Chemistry
title Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
spellingShingle Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
Santos, B. L.
Rutin
Flavonoid
Gliomas
Apoptosis
Differentiation
P-ERK
GFAP
title_short Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
title_full Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
title_fullStr Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
title_full_unstemmed Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
title_sort Antiproliferative, proapoptotic and morphogenic effects of the flavonoid rutin on human glioblastoma cells
author Santos, B. L.
author_facet Santos, B. L.
Silva, A. R.
Pitanga, B. P. S.
Sousa, C. S.
Grangeiro, M. S.
Fragomeni, B. O.
Coelho, P. L. C.
Oliveira, M. N.
Menezes Filho, N. J.
Costa, Maria de Fátima Dias
El-Bachá, Ramon dos Santos
Velozo, Eudes da Silva
Sampaio, G. P.
Freire, S. M.
Tardy, Marcienne Bloch
Costa, Silvia Lima
author_role author
author2 Silva, A. R.
Pitanga, B. P. S.
Sousa, C. S.
Grangeiro, M. S.
Fragomeni, B. O.
Coelho, P. L. C.
Oliveira, M. N.
Menezes Filho, N. J.
Costa, Maria de Fátima Dias
El-Bachá, Ramon dos Santos
Velozo, Eudes da Silva
Sampaio, G. P.
Freire, S. M.
Tardy, Marcienne Bloch
Costa, Silvia Lima
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, B. L.
Silva, A. R.
Pitanga, B. P. S.
Sousa, C. S.
Grangeiro, M. S.
Fragomeni, B. O.
Coelho, P. L. C.
Oliveira, M. N.
Menezes Filho, N. J.
Costa, Maria de Fátima Dias
El-Bachá, Ramon dos Santos
Velozo, Eudes da Silva
Sampaio, G. P.
Freire, S. M.
Tardy, Marcienne Bloch
Costa, Silvia Lima
Santos, B. L.
Silva, A. R.
Pitanga, B. P. S.
Sousa, C. S.
Grangeiro, M. S.
Fragomeni, B. O.
Coelho, P. L. C.
Oliveira, M. N.
Menezes Filho, N. J.
Costa, Maria de Fátima Dias
El-Bachá, Ramon dos Santos
Velozo, Eudes da Silva
Sampaio, G. P.
Freire, S. M.
Tardy, Marcienne Bloch
Costa, Silvia Lima
dc.subject.por.fl_str_mv Rutin
Flavonoid
Gliomas
Apoptosis
Differentiation
P-ERK
GFAP
topic Rutin
Flavonoid
Gliomas
Apoptosis
Differentiation
P-ERK
GFAP
description Texto completo: acesso restrito. p. 404–411
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-03-13T18:23:05Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14748
dc.identifier.issn.none.fl_str_mv 0308-8146
dc.identifier.number.pt_BR.fl_str_mv v. 127, n. 2
identifier_str_mv 0308-8146
v. 127, n. 2
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dc.language.iso.fl_str_mv eng
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