The oligomeric state of thyroid receptor regulates hormone binding kinetics

Detalhes bibliográficos
Autor(a) principal: Lima, Suzana Teles Cunha
Data de Publicação: 2011
Outros Autores: Rodrigues, Edson D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16306
Resumo: Texto completo: acesso restrito. p. 125-134
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spelling Lima, Suzana Teles CunhaRodrigues, Edson D.Lima, Suzana Teles CunhaRodrigues, Edson D.2014-10-03T19:22:22Z20110022-0795http://repositorio.ufba.br/ri/handle/ri/16306v. 210Texto completo: acesso restrito. p. 125-134We previously reported that mutations in the thyroid hormone receptor (TR) surface that mediates dimer and heterodimer formation do not alter affinity for cognate hormone (triiodothyronine (T3)) yet dramatically enhance T3 association and dissociation rates. This study aimed to show that TR oligomeric state influences binding and dissociation kinetics. We performed binding assays using marked hormone (125I-T3) and TRs expressed and purified by different methods. We find that T3 associates with TRs with biphasic kinetics in solution; a rapid step (half-life ±0.1 h) followed by a slower second step (half-life ±5 h) and that purification of monomers suggests that biphasic kinetics are due to the presence of monomers and dimers in our preparations. In support of this idea, incubation of TR ligand binding domain monomers with corepressor peptide induces dimer formation and decreases association rates and T3 binds to, and dissociates from, a TRβ mutant that only forms dimers (TRβD355R) with slow single-phase kinetics. In addition, heterodimer formation with retinoid X receptors also influences ligand binding kinetics. Together, these results suggest that the dimer/heterodimer surface is allosterically coupled to the hormone binding pocket and that different interactions at this surface exert different effects on ligand binding that may be relevant for TR actions in the cell.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-15T13:38:02Z No. of bitstreams: 1 Suzana T Cunha Lima.pdf: 270164 bytes, checksum: 47e8f3e8da2cae90fd284eb37eb67bc2 (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-10-03T19:22:22Z (GMT) No. of bitstreams: 1 Suzana T Cunha Lima.pdf: 270164 bytes, checksum: 47e8f3e8da2cae90fd284eb37eb67bc2 (MD5)Made available in DSpace on 2014-10-03T19:22:22Z (GMT). No. of bitstreams: 1 Suzana T Cunha Lima.pdf: 270164 bytes, checksum: 47e8f3e8da2cae90fd284eb37eb67bc2 (MD5) Previous issue date: 2011http://dx.doi.org/ 10.1530/JOE-11-0019reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAThe oligomeric state of thyroid receptor regulates hormone binding kineticsJournal of Endocrinologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengLICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16306/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52ORIGINALSuzana T Cunha Lima.pdfSuzana T Cunha Lima.pdfapplication/pdf270164https://repositorio.ufba.br/bitstream/ri/16306/1/Suzana%20T%20Cunha%20Lima.pdf47e8f3e8da2cae90fd284eb37eb67bc2MD51TEXTSuzana T Cunha Lima.pdf.txtSuzana T Cunha Lima.pdf.txtExtracted texttext/plain53607https://repositorio.ufba.br/bitstream/ri/16306/3/Suzana%20T%20Cunha%20Lima.pdf.txt98799a990817f4ede726780fab8b1bb0MD53ri/163062022-07-05 14:03:43.355oai:repositorio.ufba.br:ri/16306VGVybW8gZGUgTGljZW4/YSwgbj9vIGV4Y2x1c2l2bywgcGFyYSBvIGRlcD9zaXRvIG5vIFJlcG9zaXQ/cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZCQS4KCiBQZWxvIHByb2Nlc3NvIGRlIHN1Ym1pc3M/byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldSByZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIAplc3NlIHRlcm1vIGRlIGxpY2VuP2EsIGNvbmNlZGUgYW8gUmVwb3NpdD9yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSAKbyBkaXJlaXRvIGRlIG1hbnRlciB1bWEgYz9waWEgZW0gc2V1IHJlcG9zaXQ/cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2YT8/by4gCkVzc2VzIHRlcm1vcywgbj9vIGV4Y2x1c2l2b3MsIG1hbnQ/bSBvcyBkaXJlaXRvcyBkZSBhdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIApjb21vIHBhcnRlIGRvIGFjZXJ2byBpbnRlbGVjdHVhbCBkZXNzYSBVbml2ZXJzaWRhZGUuCgogUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVpPz9vLCBlc3NlIHRlcm1vIGRlIGxpY2VuP2EgCmVudGVuZGUgcXVlOgoKIE1hbnRlbmRvIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCByZXBhc3NhZG9zIGEgdGVyY2Vpcm9zLCBlbSBjYXNvIGRlIHB1YmxpY2E/P2VzLCBvIHJlcG9zaXQ/cmlvCnBvZGUgcmVzdHJpbmdpciBvIGFjZXNzbyBhbyB0ZXh0byBpbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1hPz9lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHU/P28gY2llbnQ/ZmljYSBjb20gCmFzIHJlc3RyaT8/ZXMgaW1wb3N0YXMgcGVsb3MgZWRpdG9yZXMgZGUgcGVyaT9kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2E/P2VzIHNlbSBpbmljaWF0aXZhcyBxdWUgc2VndWVtIGEgcG9sP3RpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVwP3NpdG9zIApjb21wdWxzP3Jpb3MgbmVzc2UgcmVwb3NpdD9yaW8gbWFudD9tIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudD9tIGFjZXNzbyBpcnJlc3RyaXRvIAphbyBtZXRhZGFkb3MgZSB0ZXh0byBjb21wbGV0by4gQXNzaW0sIGEgYWNlaXRhPz9vIGRlc3NlIHRlcm1vIG4/byBuZWNlc3NpdGEgZGUgY29uc2VudGltZW50bwogcG9yIHBhcnRlIGRlIGF1dG9yZXMvZGV0ZW50b3JlcyBkb3MgZGlyZWl0b3MsIHBvciBlc3RhcmVtIGVtIGluaWNpYXRpdmFzIGRlIGFjZXNzbyBhYmVydG8uCg==Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:43Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv The oligomeric state of thyroid receptor regulates hormone binding kinetics
dc.title.alternative.pt_BR.fl_str_mv Journal of Endocrinology
title The oligomeric state of thyroid receptor regulates hormone binding kinetics
spellingShingle The oligomeric state of thyroid receptor regulates hormone binding kinetics
Lima, Suzana Teles Cunha
title_short The oligomeric state of thyroid receptor regulates hormone binding kinetics
title_full The oligomeric state of thyroid receptor regulates hormone binding kinetics
title_fullStr The oligomeric state of thyroid receptor regulates hormone binding kinetics
title_full_unstemmed The oligomeric state of thyroid receptor regulates hormone binding kinetics
title_sort The oligomeric state of thyroid receptor regulates hormone binding kinetics
author Lima, Suzana Teles Cunha
author_facet Lima, Suzana Teles Cunha
Rodrigues, Edson D.
author_role author
author2 Rodrigues, Edson D.
author2_role author
dc.contributor.author.fl_str_mv Lima, Suzana Teles Cunha
Rodrigues, Edson D.
Lima, Suzana Teles Cunha
Rodrigues, Edson D.
description Texto completo: acesso restrito. p. 125-134
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-03T19:22:22Z
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16306
dc.identifier.issn.none.fl_str_mv 0022-0795
dc.identifier.number.pt_BR.fl_str_mv v. 210
identifier_str_mv 0022-0795
v. 210
url http://repositorio.ufba.br/ri/handle/ri/16306
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