Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/15999 |
Resumo: | Texto completo: acesso restrito. p.197–205 |
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Rodrigues, Cleusa Alves TheodoroBatista, Luís Fábio da SilvaTeixeira, Márcia Cristina AquinoPereira, Andréa MendesSantos, Patrícia Oliveira MeiraOliveira, Geraldo Gileno de SáFreitas, Luiz Antônio Rodrigues deVeras, Patrícia Sampaio TavaresRodrigues, Cleusa Alves TheodoroBatista, Luís Fábio da SilvaTeixeira, Márcia Cristina AquinoPereira, Andréa MendesSantos, Patrícia Oliveira MeiraOliveira, Geraldo Gileno de SáFreitas, Luiz Antônio Rodrigues deVeras, Patrícia Sampaio Tavares2014-09-09T16:08:42Z20070304-4017http://repositorio.ufba.br/ri/handle/ri/15999v. 143, n. 3-4Texto completo: acesso restrito. p.197–205Leishmania chagasi is the causative agent of visceral leishmaniasis in both humans and dogs in the New World. The dog is the main domestic reservoir and its infection displays different clinical presentations, from asymptomatic to severe disease. Macrophages play an important role in the control of Leishmania infection. Although it is not an area of intense study, some data suggest a role for canine macrophages in parasite killing by a NO-dependent mechanism. It has been proposed that control of human disease could be possible with the development of an effective vaccine against canine visceral leishmaniasis. Development of a rapid in vitro test to predict animal responses to Leishmania infection or vaccination should be helpful. In this study, an in vitro model was established to test whether peripheral blood mononuclear cell (PBMC) supernatants from dogs immunized with promastigote lysates and infected with L. chagasi promastigotes could stimulate macrophages from healthy dogs in order to control parasite infection. PBMC from a majority of the immunized and experimentally infected dogs expressed IFN-γ mRNA and secreted IFN-γ when stimulated with soluble L. chagasi antigen (SLA) in vitro. Additionally, the supernatants from stimulated PBMC were able to reduce the percentage of infected donor macrophages. The results also indicate that parasite killing in this system is dependent on NO, since aminoguanidine (AMG) reversed this effect. This in vitro test appears to be useful for screening animal responses to parasite inoculation as well as studying the lymphocyte effector mechanisms involved in pathogen killing by canine macrophages.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-03-11T13:18:46Z No. of bitstreams: 1 1-s2.0-S030440170600522X-main.pdf: 361395 bytes, checksum: c6b1028ecc0c6970fc16323d9f1267cf (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2014-09-09T16:08:42Z (GMT) No. of bitstreams: 1 1-s2.0-S030440170600522X-main.pdf: 361395 bytes, checksum: c6b1028ecc0c6970fc16323d9f1267cf (MD5)Made available in DSpace on 2014-09-09T16:08:42Z (GMT). No. of bitstreams: 1 1-s2.0-S030440170600522X-main.pdf: 361395 bytes, checksum: c6b1028ecc0c6970fc16323d9f1267cf (MD5) Previous issue date: 2007http://dx.doi.org/10.1016/j.vetpar.2006.09.003reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmania chagasiDog macrophageParasite killingScreening in vitro testPeripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitroVeterinary Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S030440170600522X-main.pdf1-s2.0-S030440170600522X-main.pdfapplication/pdf361395https://repositorio.ufba.br/bitstream/ri/15999/1/1-s2.0-S030440170600522X-main.pdfc6b1028ecc0c6970fc16323d9f1267cfMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15999/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT1-s2.0-S030440170600522X-main.pdf.txt1-s2.0-S030440170600522X-main.pdf.txtExtracted texttext/plain37956https://repositorio.ufba.br/bitstream/ri/15999/3/1-s2.0-S030440170600522X-main.pdf.txt1c815b22a3dd23893b90f5c70f89c192MD53ri/159992022-07-05 14:03:21.628oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:21Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| dc.title.alternative.pt_BR.fl_str_mv |
Veterinary Parasitology |
| title |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| spellingShingle |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro Rodrigues, Cleusa Alves Theodoro Leishmania chagasi Dog macrophage Parasite killing Screening in vitro test |
| title_short |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| title_full |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| title_fullStr |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| title_full_unstemmed |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| title_sort |
Peripheral blood mononuclear cell supernatants from asymptomatic dogs immunized and experimentally challenged with Leishmania chagasi can stimulate canine macrophages to reduce infection in vitro |
| author |
Rodrigues, Cleusa Alves Theodoro |
| author_facet |
Rodrigues, Cleusa Alves Theodoro Batista, Luís Fábio da Silva Teixeira, Márcia Cristina Aquino Pereira, Andréa Mendes Santos, Patrícia Oliveira Meira Oliveira, Geraldo Gileno de Sá Freitas, Luiz Antônio Rodrigues de Veras, Patrícia Sampaio Tavares |
| author_role |
author |
| author2 |
Batista, Luís Fábio da Silva Teixeira, Márcia Cristina Aquino Pereira, Andréa Mendes Santos, Patrícia Oliveira Meira Oliveira, Geraldo Gileno de Sá Freitas, Luiz Antônio Rodrigues de Veras, Patrícia Sampaio Tavares |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rodrigues, Cleusa Alves Theodoro Batista, Luís Fábio da Silva Teixeira, Márcia Cristina Aquino Pereira, Andréa Mendes Santos, Patrícia Oliveira Meira Oliveira, Geraldo Gileno de Sá Freitas, Luiz Antônio Rodrigues de Veras, Patrícia Sampaio Tavares Rodrigues, Cleusa Alves Theodoro Batista, Luís Fábio da Silva Teixeira, Márcia Cristina Aquino Pereira, Andréa Mendes Santos, Patrícia Oliveira Meira Oliveira, Geraldo Gileno de Sá Freitas, Luiz Antônio Rodrigues de Veras, Patrícia Sampaio Tavares |
| dc.subject.por.fl_str_mv |
Leishmania chagasi Dog macrophage Parasite killing Screening in vitro test |
| topic |
Leishmania chagasi Dog macrophage Parasite killing Screening in vitro test |
| description |
Texto completo: acesso restrito. p.197–205 |
| publishDate |
2007 |
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2007 |
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2014-09-09T16:08:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://repositorio.ufba.br/ri/handle/ri/15999 |
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0304-4017 |
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v. 143, n. 3-4 |
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0304-4017 v. 143, n. 3-4 |
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http://repositorio.ufba.br/ri/handle/ri/15999 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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