Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry

Detalhes bibliográficos
Autor(a) principal: Portela, Jicarla Gusmão
Data de Publicação: 2004
Outros Autores: Costa, Antonio Celso Spinola, Teixeira, Leonardo Sena Gomes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7887
Resumo: Texto completo: acesso restrito. p. 543-549
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spelling Portela, Jicarla GusmãoCosta, Antonio Celso SpinolaTeixeira, Leonardo Sena GomesPortela, Jicarla GusmãoCosta, Antonio Celso SpinolaTeixeira, Leonardo Sena Gomes2013-01-16T12:32:40Z20040731-7085http://www.repositorio.ufba.br/ri/handle/ri/7887v. 34, n. 3Texto completo: acesso restrito. p. 543-549In this work, a new solid phase spectrophotometric method in association with flow injection analysis for Vitamin B6 (pyridoxine) determination has been developed with direct measurement of light-absorption in C18 material. In the developed method, successive passage of the complex, previously formed in the flowing stream, and eluent through the flow cell and continuous monitoring of the process provided the analytical information needed to determine pyridoxine. Pharmaceutical samples containing Vitamin B6 were previously dissolved in 0.1 mol l−1 phosphate buffer solution (pH 7.5) and a sample volume of 235 μl was injected directly into carrier stream consisting of a mixture of methanol and 0.1 mol l−1 phosphate buffer solution adjusted to pH 7.0 (1+1, v/v). The blue indophenol dye produced from the reaction between pyridoxine and N,N-diethyl p-phenylenediamine after oxidation by potassium hexacyanoferrate(III) was quantitatively retained on C18 support and the spectrophotometric detection was performed simultaneously at 633 nm. The retained complex was quickly eluted from C18 material with the eluent stream consisting of a mixture of methanol and 0.01 mol l−1 HCl (6+4, v/v). The results showed that the proposed method is simple, rapid and the analytical response is linear in the concentration range of 0.5–10 and 0.2–4 mg l−1 using 235 and 860 μl of sample, respectively. The limits of detection are 0.15 and 0.060 mg l−1 and the R.S.D. are 3.6% (at 2 mg l−1 level) and 4.0% (at 1 mg l−1 level) using sample volume of 235 and 860 μl, respectively. The system presented an analytical throughput of 15 determinations per hour when a sample volume of 235 μl was utilized. The procedure was successfully applied to the determination of Vitamin B6 in pharmaceutical formulations containing vitamins of B group and others active principles such as Vitamin C and minerals.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2013-01-16T12:32:39Z No. of bitstreams: 1 1-s2.0-S0731708503005892-main.pdf: 91967 bytes, checksum: fcb935d26638d1987a35d0e45e0930d6 (MD5)Made available in DSpace on 2013-01-16T12:32:40Z (GMT). No. of bitstreams: 1 1-s2.0-S0731708503005892-main.pdf: 91967 bytes, checksum: fcb935d26638d1987a35d0e45e0930d6 (MD5) Previous issue date: 2004Elservierhttp://dx.doi.org/10.1016/S0731-7085(03)00589-2reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAVitamin B6Solid phase spectrophotometryFlow injection analysisDetermination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometryJournal of Pharmaceutical and Biomedical Analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S0731708503005892-main.pdf1-s2.0-S0731708503005892-main.pdfapplication/pdf91967https://repositorio.ufba.br/bitstream/ri/7887/1/1-s2.0-S0731708503005892-main.pdffcb935d26638d1987a35d0e45e0930d6MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7887/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S0731708503005892-main.pdf.txt1-s2.0-S0731708503005892-main.pdf.txtExtracted texttext/plain23806https://repositorio.ufba.br/bitstream/ri/7887/3/1-s2.0-S0731708503005892-main.pdf.txtae6330e687e7eb60647887f093075d62MD53ri/78872022-08-08 13:19:56.02oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:19:56Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
dc.title.alternative.pt_BR.fl_str_mv Journal of Pharmaceutical and Biomedical Analysis
title Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
spellingShingle Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
Portela, Jicarla Gusmão
Vitamin B6
Solid phase spectrophotometry
Flow injection analysis
title_short Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
title_full Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
title_fullStr Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
title_full_unstemmed Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
title_sort Determination of Vitamin B6 in pharmaceutical formulations by flow injection-solid phase spectrophotometry
author Portela, Jicarla Gusmão
author_facet Portela, Jicarla Gusmão
Costa, Antonio Celso Spinola
Teixeira, Leonardo Sena Gomes
author_role author
author2 Costa, Antonio Celso Spinola
Teixeira, Leonardo Sena Gomes
author2_role author
author
dc.contributor.author.fl_str_mv Portela, Jicarla Gusmão
Costa, Antonio Celso Spinola
Teixeira, Leonardo Sena Gomes
Portela, Jicarla Gusmão
Costa, Antonio Celso Spinola
Teixeira, Leonardo Sena Gomes
dc.subject.por.fl_str_mv Vitamin B6
Solid phase spectrophotometry
Flow injection analysis
topic Vitamin B6
Solid phase spectrophotometry
Flow injection analysis
description Texto completo: acesso restrito. p. 543-549
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2013-01-16T12:32:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7887
dc.identifier.issn.none.fl_str_mv 0731-7085
dc.identifier.number.pt_BR.fl_str_mv v. 34, n. 3
identifier_str_mv 0731-7085
v. 34, n. 3
url http://www.repositorio.ufba.br/ri/handle/ri/7887
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elservier
publisher.none.fl_str_mv Elservier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/S0731-7085(03)00589-2
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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