Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/20341 |
Resumo: | Artigo original (p.13-19) |
| id |
UFBA-2_6279bc53430d3e97797f8955d05f4179 |
|---|---|
| oai_identifier_str |
oai:repositorio.ufba.br:ri/20341 |
| network_acronym_str |
UFBA-2 |
| network_name_str |
Repositório Institucional da UFBA |
| repository_id_str |
1932 |
| spelling |
Couto, Alessanda Abdo doBarros, José Júnior França deCouceiro, José Nelson dos Santos SilvaCouto, Alessanda Abdo doBarros, José Júnior França deCouceiro, José Nelson dos Santos Silva2016-09-13T15:08:36Z2016-09-13T15:08:36Z2004COUTO, A. A. do. Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 13-19, jan./jun. 2004.2236-5222http://repositorio.ufba.br/ri/handle/ri/20341v.3, n.1Artigo original (p.13-19)Influenza virus sialidase develops an essential activity on cellular glycoproteins, then permitting the dissemination of the virus infections by preventing virus-virus self aggregation and virus-cell rebinding. Two purified variant samples of influenza A/Memphis/102/72 (H3N2) viruses, which are recognized for their receptor-binding activity to a-2,6 or a- 2,3-sialyllactose structures, were analysed for their sialidase activity on different natural and artificial substrates. The M1/ 5 sample exhibited higher sialidase activity on fetuin (O.D.=0.226), MPN (O.D.=0.110) and human erythrocytes (10,240 HAU/ml), while the activity of the M1/5HS8 sample on these substrates was expressed by O.D.=0.129, O.D.=0.065 and 2,560 HAU/ml when using fetuin, MPN and human erythrocytes as substrates, respectively. However, the M1/5HS8 sample showed more significative sialidase activity on mucin when compared to the M1/5 sample: the enzyme activity of first sample was responsible for liberation of 3.5 nmol of free sialic acids while the last one produced 16.5 nmol of free sialic acidsSubmitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-13T15:08:36Z No. of bitstreams: 1 R. Ci. méd. biol., v.3, n. 1-2004.pdf: 68489 bytes, checksum: ec482474a61fcf3e7bff2d7bf75ee293 (MD5)Made available in DSpace on 2016-09-13T15:08:36Z (GMT). No. of bitstreams: 1 R. Ci. méd. biol., v.3, n. 1-2004.pdf: 68489 bytes, checksum: ec482474a61fcf3e7bff2d7bf75ee293 (MD5) Previous issue date: 2004-01SalvadorInstituto de Ciências da Saúde/ Universidade Federal da Bahiahttp://www.portalseer.ufba.br/index.php/cmbio/issue/view/498/showTocreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAInfluenza virus.Receptor-binding variants.Neuraminidase (NA).Natural and artificial substrates.Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substratesRevista de Ciências Médicas e Biológicasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessporORIGINALR. Ci. méd. biol., v.3, n. 1-2004.pdfR. Ci. méd. biol., v.3, n. 1-2004.pdfapplication/pdf68489https://repositorio.ufba.br/bitstream/ri/20341/1/R.%20%20Ci.%20m%c3%a9d.%20biol.%2c%20v.3%2c%20n.%201-2004.pdfec482474a61fcf3e7bff2d7bf75ee293MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/20341/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTR. Ci. méd. biol., v.3, n. 1-2004.pdf.txtR. Ci. méd. biol., v.3, n. 1-2004.pdf.txtExtracted texttext/plain21050https://repositorio.ufba.br/bitstream/ri/20341/3/R.%20%20Ci.%20m%c3%a9d.%20biol.%2c%20v.3%2c%20n.%201-2004.pdf.txt869d9e19fa35c1a7a1e743d19b619621MD53ri/203412022-10-10 15:51:26.964oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-10-10T18:51:26Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| dc.title.alternative.pt_BR.fl_str_mv |
Revista de Ciências Médicas e Biológicas |
| title |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| spellingShingle |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates Couto, Alessanda Abdo do Influenza virus. Receptor-binding variants. Neuraminidase (NA). Natural and artificial substrates. |
| title_short |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| title_full |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| title_fullStr |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| title_full_unstemmed |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| title_sort |
Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates |
| author |
Couto, Alessanda Abdo do |
| author_facet |
Couto, Alessanda Abdo do Barros, José Júnior França de Couceiro, José Nelson dos Santos Silva |
| author_role |
author |
| author2 |
Barros, José Júnior França de Couceiro, José Nelson dos Santos Silva |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Couto, Alessanda Abdo do Barros, José Júnior França de Couceiro, José Nelson dos Santos Silva Couto, Alessanda Abdo do Barros, José Júnior França de Couceiro, José Nelson dos Santos Silva |
| dc.subject.por.fl_str_mv |
Influenza virus. Receptor-binding variants. Neuraminidase (NA). Natural and artificial substrates. |
| topic |
Influenza virus. Receptor-binding variants. Neuraminidase (NA). Natural and artificial substrates. |
| description |
Artigo original (p.13-19) |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004 |
| dc.date.accessioned.fl_str_mv |
2016-09-13T15:08:36Z |
| dc.date.available.fl_str_mv |
2016-09-13T15:08:36Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
COUTO, A. A. do. Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 13-19, jan./jun. 2004. |
| dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/20341 |
| dc.identifier.issn.none.fl_str_mv |
2236-5222 |
| dc.identifier.number.pt_BR.fl_str_mv |
v.3, n.1 |
| identifier_str_mv |
COUTO, A. A. do. Receptor-binding variants of H3N2 influenza A viruses: characterization of their sialidase activity towards different substrates. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 13-19, jan./jun. 2004. 2236-5222 v.3, n.1 |
| url |
http://repositorio.ufba.br/ri/handle/ri/20341 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Instituto de Ciências da Saúde/ Universidade Federal da Bahia |
| publisher.none.fl_str_mv |
Instituto de Ciências da Saúde/ Universidade Federal da Bahia |
| dc.source.pt_BR.fl_str_mv |
http://www.portalseer.ufba.br/index.php/cmbio/issue/view/498/showToc |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
| instname_str |
Universidade Federal da Bahia (UFBA) |
| instacron_str |
UFBA |
| institution |
UFBA |
| reponame_str |
Repositório Institucional da UFBA |
| collection |
Repositório Institucional da UFBA |
| bitstream.url.fl_str_mv |
https://repositorio.ufba.br/bitstream/ri/20341/1/R.%20%20Ci.%20m%c3%a9d.%20biol.%2c%20v.3%2c%20n.%201-2004.pdf https://repositorio.ufba.br/bitstream/ri/20341/2/license.txt https://repositorio.ufba.br/bitstream/ri/20341/3/R.%20%20Ci.%20m%c3%a9d.%20biol.%2c%20v.3%2c%20n.%201-2004.pdf.txt |
| bitstream.checksum.fl_str_mv |
ec482474a61fcf3e7bff2d7bf75ee293 ff6eaa8b858ea317fded99f125f5fcd0 869d9e19fa35c1a7a1e743d19b619621 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA) |
| repository.mail.fl_str_mv |
|
| _version_ |
1808459500973195264 |