Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans

Detalhes bibliográficos
Autor(a) principal: Trindade, Soraya Castro
Data de Publicação: 2012
Outros Autores: Olczak, T., Gomes Filho, I. S., Costa, L. F. Moura, Cerqueira, E. M. M., Galdino Neto, Milton, Alves, Heidiane, Carvalho Filho, Paulo Cirino de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15692
Resumo: Texto completo: acesso restrito. p. 27–32
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spelling Trindade, Soraya CastroOlczak, T.Gomes Filho, I. S.Costa, L. F. MouraCerqueira, E. M. M.Galdino Neto, MiltonAlves, HeidianeCarvalho Filho, Paulo Cirino deTrindade, Soraya CastroOlczak, T.Gomes Filho, I. S.Costa, L. F. MouraCerqueira, E. M. M.Galdino Neto, MiltonAlves, HeidianeCarvalho Filho, Paulo Cirino de2014-08-25T14:26:45Z20120022-3484http://repositorio.ufba.br/ri/handle/ri/15692v. 47, n. 1Texto completo: acesso restrito. p. 27–32Background and Objective: Porphyromonas gingivalis, an anaerobic gram-negative bacterium, is associated with chronic periodontitis. This study was undertaken to evaluate the production of interleukin (IL)-1β, IL-8 and IL-10 by human peripheral blood mononuclear cells (PBMC) stimulated with P. gingivalis antigens and to assess the levels of serum immunoglobulin (Ig)G, IgA and IgG subclasses raised against P. gingivalis HmuY protein. Material and Methods:  PBMC from patients with chronic periodontitis (CP) and from nonperiodontitis (NP) control subjects were stimulated with P. gingivalis antigens, and the cytokine levels in the culture supernatants were determined by ELISA. The specificity of serum antibodies raised against HmuY was analyzed by Western blotting and by ELISA. Results:  Compared with the NP controls, the CP patients produced higher levels of total serum IgG and IgG1 specific for P. gingivalis HmuY. No differences were found between CP and NP groups in the production of IL-1β and IL-8 by PBMC stimulated with total P. gingivalis antigens. Only P. gingivalis lipopolysaccharide (LPS) induced higher levels of IL-10 in the CP group. Higher levels of IL-1β and IL-10 were induced by HmuY than by other antigens derived from the wild-type P. gingivalis strains. In contrast, total antigens derived from the hmuY-deletion mutant strain induced the production of significantly higher levels of IL-8 and significantly lower levels of IL-1β. Conclusion:  Our data suggest that P. gingivalis HmuY may be considered an immunogenic protein associated with host–pathogen interactions.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-08-25T14:26:45Z No. of bitstreams: 1 S. C. Trindade.pdf: 247017 bytes, checksum: cd5ba94528cb482345dfa2f397fc8eb9 (MD5)Made available in DSpace on 2014-08-25T14:26:45Z (GMT). No. of bitstreams: 1 S. C. Trindade.pdf: 247017 bytes, checksum: cd5ba94528cb482345dfa2f397fc8eb9 (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1111/j.1600-0765.2011.01401.xreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAChronic periodontitisCytokinesHmuYImmunoglobulin GPorphyromonas gingivalisInduction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humansJournal of Periodontal Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALS. C. Trindade.pdfS. C. Trindade.pdfapplication/pdf247017https://repositorio.ufba.br/bitstream/ri/15692/1/S.%20C.%20Trindade.pdfcd5ba94528cb482345dfa2f397fc8eb9MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15692/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTS. C. Trindade.pdf.txtS. C. 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dc.title.pt_BR.fl_str_mv Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
dc.title.alternative.pt_BR.fl_str_mv Journal of Periodontal Research
title Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
spellingShingle Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
Trindade, Soraya Castro
Chronic periodontitis
Cytokines
HmuY
Immunoglobulin G
Porphyromonas gingivalis
title_short Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
title_full Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
title_fullStr Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
title_full_unstemmed Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
title_sort Induction of interleukin (IL)-1β, IL-10, IL-8 and immunoglobulin G by Porphyromonas gingivalis HmuY in humans
author Trindade, Soraya Castro
author_facet Trindade, Soraya Castro
Olczak, T.
Gomes Filho, I. S.
Costa, L. F. Moura
Cerqueira, E. M. M.
Galdino Neto, Milton
Alves, Heidiane
Carvalho Filho, Paulo Cirino de
author_role author
author2 Olczak, T.
Gomes Filho, I. S.
Costa, L. F. Moura
Cerqueira, E. M. M.
Galdino Neto, Milton
Alves, Heidiane
Carvalho Filho, Paulo Cirino de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Trindade, Soraya Castro
Olczak, T.
Gomes Filho, I. S.
Costa, L. F. Moura
Cerqueira, E. M. M.
Galdino Neto, Milton
Alves, Heidiane
Carvalho Filho, Paulo Cirino de
Trindade, Soraya Castro
Olczak, T.
Gomes Filho, I. S.
Costa, L. F. Moura
Cerqueira, E. M. M.
Galdino Neto, Milton
Alves, Heidiane
Carvalho Filho, Paulo Cirino de
dc.subject.por.fl_str_mv Chronic periodontitis
Cytokines
HmuY
Immunoglobulin G
Porphyromonas gingivalis
topic Chronic periodontitis
Cytokines
HmuY
Immunoglobulin G
Porphyromonas gingivalis
description Texto completo: acesso restrito. p. 27–32
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-08-25T14:26:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15692
dc.identifier.issn.none.fl_str_mv 0022-3484
dc.identifier.number.pt_BR.fl_str_mv v. 47, n. 1
identifier_str_mv 0022-3484
v. 47, n. 1
url http://repositorio.ufba.br/ri/handle/ri/15692
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1111/j.1600-0765.2011.01401.x
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