Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis

Detalhes bibliográficos
Autor(a) principal: Wu, Henry M.
Data de Publicação: 2013
Outros Autores: Cordeiro, Soraia Machado, Harcourt, Brian H, Carvalho, Maria da Gloria S, Azevedo, Jailton, Oliveira, Tainara Queiroz, Leite, Mariela C, Salgado, Kátia M, Reis, Mitermayer Galvão dos, Plikaytis, Brian D., Clark, Thomas A, Mayer, Leonard W, Ko, Albert Icksang, Martin, Stacey W, Reis, Joice Neves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7495
Resumo: National Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USA
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spelling Wu, Henry M.Cordeiro, Soraia MachadoHarcourt, Brian HCarvalho, Maria da Gloria SAzevedo, JailtonOliveira, Tainara QueirozLeite, Mariela CSalgado, Kátia MReis, Mitermayer Galvão dosPlikaytis, Brian D.Clark, Thomas AMayer, Leonard WKo, Albert IcksangMartin, Stacey WReis, Joice Neves2014-04-04T13:45:45Z2014-04-04T13:45:45Z2013WU, H. M. et al. Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis. BMC Infectious Disease, v. 13, p. 26, 2013.1471-2334https://www.arca.fiocruz.br/handle/icict/749510.1186/1471-2334-13-26.engBioMed CentralAccuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilNational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USANational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFederal University of Bahia. Salvador, BA, BrasilFederal University of Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilNational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USANational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USANational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. New York, NY, USANational Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USAFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Federal University of Bahia. Salvador, BA, BrasilBACKGROUND: Although cerebrospinal fluid (CSF) culture is the diagnostic reference standard for bacterial meningitis, its sensitivity is limited, particularly when antibiotics were previously administered. CSF Gram staining and real-time PCR are theoretically less affected by antibiotics; however, it is difficult to evaluate these tests with an imperfect reference standard. METHODS AND FINDINGS: CSF from patients with suspected meningitis from Salvador, Brazil were tested with culture, Gram stain, and real-time PCR using S. pneumoniae, N. meningitidis, and H. influenzae specific primers and probes. An antibiotic detection disk bioassay was used to test for the presence of antibiotic activity in CSF. The diagnostic accuracy of tests were evaluated using multiple methods, including direct evaluation of Gram stain and real-time PCR against CSF culture, evaluation of real-time PCR against a composite reference standard, and latent class analysis modeling to evaluate all three tests simultaneously. RESULTS: Among 451 CSF specimens, 80 (17.7%) had culture isolation of one of the three pathogens (40 S. pneumoniae, 36 N. meningitidis, and 4 H. influenzae), and 113 (25.1%) were real-time PCR positive (51 S. pneumoniae, 57 N. meningitidis, and 5 H. influenzae). Compared to culture, real-time PCR sensitivity and specificity were 95.0% and 90.0%, respectively. In a latent class analysis model, the sensitivity and specificity estimates were: culture, 81.3% and 99.7%; Gram stain, 98.2% and 98.7%; and real-time PCR, 95.7% and 94.3%, respectively. Gram stain and real-time PCR sensitivity did not change significantly when there was antibiotic activity in the CSF. CONCLUSION: Real-time PCR and Gram stain were highly accurate in diagnosing meningitis caused by S. pneumoniae, N. meningitidis, and H. influenzae, though there were few cases of H. influenzae. Furthermore, real-time PCR and Gram staining were less affected by antibiotic presence and might be useful when antibiotics were previously administered. Gram staining, which is inexpensive and commonly available, should be encouraged in all clinical settings.Bacterial meningitisDiagnostic test evaluationReal-time PCRStreptococcus pneumoniaeNeisseria meningitidisHaemophilus influenzaeHaemophilus influenzae/genéticaMeningites Bacterianas/diagnósticoNeisseria meningitidis/genéticaStreptococcus pneumoniae/genéticaAdolescenteAdultoIdosoLíquido Cefalorraquidiano/microbiologiaCriançaPré-EscolarVioleta de GencianaHaemophilus influenzae/isolamento & purificaçãoHumanosLactenteRecém-NascidoMeia-IdadeNeisseria meningitidis/isolamento & purificaçãoFenazinasReação em Cadeia da Polimerase em Tempo RealSensibilidade e EspecificidadeStreptococcus pneumoniae/isolamento & purificaçãoAdulto Joveminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALWu HM Accuracy of real-time....pdfWu HM Accuracy of real-time....pdfapplication/pdf281795https://www.arca.fiocruz.br/bitstream/icict/7495/1/Wu%20HM%20Accuracy%20of%20real-time....pdf594f3384ea132f663567fb63c1ab1d4eMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7495/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTWu HM Accuracy of real-time....pdf.txtWu HM Accuracy of real-time....pdf.txtExtracted texttext/plain48251https://www.arca.fiocruz.br/bitstream/icict/7495/5/Wu%20HM%20Accuracy%20of%20real-time....pdf.txt2b2636c7535f19b48e289871097577c4MD55THUMBNAILWu HM Accuracy of real-time....pdf.jpgWu HM Accuracy of real-time....pdf.jpgGenerated Thumbnailimage/jpeg2040https://www.arca.fiocruz.br/bitstream/icict/7495/4/Wu%20HM%20Accuracy%20of%20real-time....pdf.jpg4caa89ed4d1080f18cfd7dd1789a64f6MD54icict/74952023-03-15 14:33:14.187oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
spellingShingle Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
Wu, Henry M.
Bacterial meningitis
Diagnostic test evaluation
Real-time PCR
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
Haemophilus influenzae/genética
Meningites Bacterianas/diagnóstico
Neisseria meningitidis/genética
Streptococcus pneumoniae/genética
Adolescente
Adulto
Idoso
Líquido Cefalorraquidiano/microbiologia
Criança
Pré-Escolar
Violeta de Genciana
Haemophilus influenzae/isolamento & purificação
Humanos
Lactente
Recém-Nascido
Meia-Idade
Neisseria meningitidis/isolamento & purificação
Fenazinas
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Streptococcus pneumoniae/isolamento & purificação
Adulto Jovem
title_short Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_full Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_fullStr Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_full_unstemmed Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_sort Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
author Wu, Henry M.
author_facet Wu, Henry M.
Cordeiro, Soraia Machado
Harcourt, Brian H
Carvalho, Maria da Gloria S
Azevedo, Jailton
Oliveira, Tainara Queiroz
Leite, Mariela C
Salgado, Kátia M
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A
Mayer, Leonard W
Ko, Albert Icksang
Martin, Stacey W
Reis, Joice Neves
author_role author
author2 Cordeiro, Soraia Machado
Harcourt, Brian H
Carvalho, Maria da Gloria S
Azevedo, Jailton
Oliveira, Tainara Queiroz
Leite, Mariela C
Salgado, Kátia M
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A
Mayer, Leonard W
Ko, Albert Icksang
Martin, Stacey W
Reis, Joice Neves
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wu, Henry M.
Cordeiro, Soraia Machado
Harcourt, Brian H
Carvalho, Maria da Gloria S
Azevedo, Jailton
Oliveira, Tainara Queiroz
Leite, Mariela C
Salgado, Kátia M
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A
Mayer, Leonard W
Ko, Albert Icksang
Martin, Stacey W
Reis, Joice Neves
dc.subject.en.pt_BR.fl_str_mv Bacterial meningitis
Diagnostic test evaluation
Real-time PCR
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
topic Bacterial meningitis
Diagnostic test evaluation
Real-time PCR
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
Haemophilus influenzae/genética
Meningites Bacterianas/diagnóstico
Neisseria meningitidis/genética
Streptococcus pneumoniae/genética
Adolescente
Adulto
Idoso
Líquido Cefalorraquidiano/microbiologia
Criança
Pré-Escolar
Violeta de Genciana
Haemophilus influenzae/isolamento & purificação
Humanos
Lactente
Recém-Nascido
Meia-Idade
Neisseria meningitidis/isolamento & purificação
Fenazinas
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Streptococcus pneumoniae/isolamento & purificação
Adulto Jovem
dc.subject.decs.pt_BR.fl_str_mv Haemophilus influenzae/genética
Meningites Bacterianas/diagnóstico
Neisseria meningitidis/genética
Streptococcus pneumoniae/genética
Adolescente
Adulto
Idoso
Líquido Cefalorraquidiano/microbiologia
Criança
Pré-Escolar
Violeta de Genciana
Haemophilus influenzae/isolamento & purificação
Humanos
Lactente
Recém-Nascido
Meia-Idade
Neisseria meningitidis/isolamento & purificação
Fenazinas
Reação em Cadeia da Polimerase em Tempo Real
Sensibilidade e Especificidade
Streptococcus pneumoniae/isolamento & purificação
Adulto Jovem
description National Center for Immunization and Respiratory Diseases CDC. Division of Bacterial Diseases. Atlanta, GA, USA
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-04-04T13:45:45Z
dc.date.available.fl_str_mv 2014-04-04T13:45:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WU, H. M. et al. Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis. BMC Infectious Disease, v. 13, p. 26, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7495
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2334-13-26.
identifier_str_mv WU, H. M. et al. Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis. BMC Infectious Disease, v. 13, p. 26, 2013.
1471-2334
10.1186/1471-2334-13-26.
url https://www.arca.fiocruz.br/handle/icict/7495
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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