The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4850 |
Resumo: | p. 75–86 |
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Silva, Ana RitaPinheiro, Alexandre MoraesSouza, Cleide dos SantosFreitas, Sandra Regina Villas Bôas deVasconcellos, V.Freire, Songeli MenezesVelozo, Eudes da SilvaTardy, Marcienne BlochEl-Bachá, Ramon dos SantosCosta, Maria de Fátima DiasCosta, Silvia LimaSilva, Ana RitaPinheiro, Alexandre MoraesSouza, Cleide dos SantosFreitas, Sandra Regina Villas Bôas deVasconcellos, V.Freire, Songeli MenezesVelozo, Eudes da SilvaTardy, Marcienne BlochEl-Bachá, Ramon dos SantosCosta, Maria de Fátima DiasCosta, Silvia Lima2011-12-14T12:21:16Z2011-12-14T12:21:16Z20081573-6822http://www.repositorio.ufba.br/ri/handle/ri/4850v.24p. 75–86Astrocyte and microglia cells play an important role in the central nervous system (CNS). They react to various external aggressions by becoming reactive and releasing neurotrophic and/or neurotoxic factors. Rutin is a flavonoid found in many plants and has been shown to have some biological activities, but its direct effects on cells of the CNS have not been well studied. To investigate its potential effects on CNS glial cells, we used both astrocyte primary cultures and astrocyte/microglia mixed primary cell cultures derived from newborn rat cortical brain. The cultures were treated for 24 h with rutin (50 or 100 μmol/L) or vehicle (0.5% dimethyl sulfoxide). Mitochondrial function on glial cells was not evidenced by the MTT test. However, an increased lactate dehydrogenase activity was detected in the culture medium of both culture systems when treated with 100 μmol/L rutin, suggesting loss of cell membrane integrity. Astrocytes exposed to 50 μmol/L rutin became reactive as revealed by glial fibrillary acidic protein (GFAP) overexpression and showed a star-like phenotype revealed by Rosenfeld’s staining. The number of activated microglia expressing OX-42 increased in the presence of rutin. A significant increase of nitric oxide (NO) was observed only in mixed cultures exposed to 100 μmol/L rutin. Enhanced TNFα release was observed in astrocyte primary cultures treated with 100 μmol/L rutin and in mixed primary cultures treated with 50 and 100 μmol/L, suggesting different sensitivity of both activated cell types. These results demonstrated that rutin affects astrocytes and microglial cells in culture and has the capacity to induce NO and TNFα production in these cells. Hence, the impact of these effects on neurons in vitro and in vivo needs to be studied.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2011-12-14T12:21:16Z No. of bitstreams: 1 05 fulltext.pdf: 504718 bytes, checksum: a98643f4459f97229dfd797b26c570ea (MD5)Made available in DSpace on 2011-12-14T12:21:16Z (GMT). No. of bitstreams: 1 05 fulltext.pdf: 504718 bytes, checksum: a98643f4459f97229dfd797b26c570ea (MD5) Previous issue date: 2008AstrocyteFlavonoidMicrogliaNORutinTNF-αThe flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell culturesCell Biology and Toxicologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINAL05 fulltext.pdf05 fulltext.pdfapplication/pdf504718https://repositorio.ufba.br/bitstream/ri/4850/1/05%20fulltext.pdfa98643f4459f97229dfd797b26c570eaMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/4850/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT05 fulltext.pdf.txt05 fulltext.pdf.txtExtracted texttext/plain45257https://repositorio.ufba.br/bitstream/ri/4850/3/05%20fulltext.pdf.txt46b0af261ad11a6d09c740e7cfdc72bfMD53ri/48502022-07-05 14:03:15.904oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:15Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| dc.title.alternative.pt_BR.fl_str_mv |
Cell Biology and Toxicology |
| title |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| spellingShingle |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures Silva, Ana Rita Astrocyte Flavonoid Microglia NO Rutin TNF-α |
| title_short |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| title_full |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| title_fullStr |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| title_full_unstemmed |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| title_sort |
The flavonoid rutin induces astrocyte and microglia activation and regulates TNF-alpha and NO release in primary glial cell cultures |
| author |
Silva, Ana Rita |
| author_facet |
Silva, Ana Rita Pinheiro, Alexandre Moraes Souza, Cleide dos Santos Freitas, Sandra Regina Villas Bôas de Vasconcellos, V. Freire, Songeli Menezes Velozo, Eudes da Silva Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Costa, Silvia Lima |
| author_role |
author |
| author2 |
Pinheiro, Alexandre Moraes Souza, Cleide dos Santos Freitas, Sandra Regina Villas Bôas de Vasconcellos, V. Freire, Songeli Menezes Velozo, Eudes da Silva Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Costa, Silvia Lima |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Ana Rita Pinheiro, Alexandre Moraes Souza, Cleide dos Santos Freitas, Sandra Regina Villas Bôas de Vasconcellos, V. Freire, Songeli Menezes Velozo, Eudes da Silva Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Costa, Silvia Lima Silva, Ana Rita Pinheiro, Alexandre Moraes Souza, Cleide dos Santos Freitas, Sandra Regina Villas Bôas de Vasconcellos, V. Freire, Songeli Menezes Velozo, Eudes da Silva Tardy, Marcienne Bloch El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Costa, Silvia Lima |
| dc.subject.por.fl_str_mv |
Astrocyte Flavonoid Microglia NO Rutin TNF-α |
| topic |
Astrocyte Flavonoid Microglia NO Rutin TNF-α |
| description |
p. 75–86 |
| publishDate |
2008 |
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2008 |
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2011-12-14T12:21:16Z |
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2011-12-14T12:21:16Z |
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1573-6822 |
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v.24 |
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1573-6822 v.24 |
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