An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5042 |
Resumo: | p.182-189 |
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Teixeira, Márcia Cristina AquinoOliveira, Geraldo Gileno de SáSantos, Patrícia Oliveira MeiraBahiense, Thiago CampanharoSilva, Virginia Maria Goes daRodrigues, Márcio SilvaLarangeira, Daniela FariasSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes deTeixeira, Márcia Cristina AquinoOliveira, Geraldo Gileno de SáSantos, Patrícia Oliveira MeiraBahiense, Thiago CampanharoSilva, Virginia Maria Goes daRodrigues, Márcio SilvaLarangeira, Daniela FariasSantos, Washington Luis Conrado dosCarvalho, Lain Carlos Pontes de2012-01-06T11:55:31Z2012-01-06T11:55:31Z2011-031678-8060http://www.repositorio.ufba.br/ri/handle/ri/5042106(2)p.182-189Domestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum/Leishmania chagasi-specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-06T11:55:31Z No. of bitstreams: 1 oc11030.pdf: 873102 bytes, checksum: 8830ca1cfc00bdbd02159e868c42fa64 (MD5)Made available in DSpace on 2012-01-06T11:55:31Z (GMT). No. of bitstreams: 1 oc11030.pdf: 873102 bytes, checksum: 8830ca1cfc00bdbd02159e868c42fa64 (MD5) Previous issue date: 2011-03Leishmania infantumLeishmania chagasidogsimmunizationcellular immune responseAn experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigensMemórias do Instituto Oswaldo Cruzinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALoc11030.pdfoc11030.pdfapplication/pdf873102https://repositorio.ufba.br/bitstream/ri/5042/1/oc11030.pdf8830ca1cfc00bdbd02159e868c42fa64MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5042/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXToc11030.pdf.txtoc11030.pdf.txtExtracted texttext/plain47118https://repositorio.ufba.br/bitstream/ri/5042/3/oc11030.pdf.txt9b436de1332113b6d0295263f9818f66MD53ri/50422022-07-05 14:03:14.75oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:14Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| dc.title.alternative.pt_BR.fl_str_mv |
Memórias do Instituto Oswaldo Cruz |
| title |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| spellingShingle |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens Teixeira, Márcia Cristina Aquino Leishmania infantum Leishmania chagasi dogs immunization cellular immune response |
| title_short |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| title_full |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| title_fullStr |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| title_full_unstemmed |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| title_sort |
An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens |
| author |
Teixeira, Márcia Cristina Aquino |
| author_facet |
Teixeira, Márcia Cristina Aquino Oliveira, Geraldo Gileno de Sá Santos, Patrícia Oliveira Meira Bahiense, Thiago Campanharo Silva, Virginia Maria Goes da Rodrigues, Márcio Silva Larangeira, Daniela Farias Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author_role |
author |
| author2 |
Oliveira, Geraldo Gileno de Sá Santos, Patrícia Oliveira Meira Bahiense, Thiago Campanharo Silva, Virginia Maria Goes da Rodrigues, Márcio Silva Larangeira, Daniela Farias Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Teixeira, Márcia Cristina Aquino Oliveira, Geraldo Gileno de Sá Santos, Patrícia Oliveira Meira Bahiense, Thiago Campanharo Silva, Virginia Maria Goes da Rodrigues, Márcio Silva Larangeira, Daniela Farias Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de Teixeira, Márcia Cristina Aquino Oliveira, Geraldo Gileno de Sá Santos, Patrícia Oliveira Meira Bahiense, Thiago Campanharo Silva, Virginia Maria Goes da Rodrigues, Márcio Silva Larangeira, Daniela Farias Santos, Washington Luis Conrado dos Carvalho, Lain Carlos Pontes de |
| dc.subject.por.fl_str_mv |
Leishmania infantum Leishmania chagasi dogs immunization cellular immune response |
| topic |
Leishmania infantum Leishmania chagasi dogs immunization cellular immune response |
| description |
p.182-189 |
| publishDate |
2011 |
| dc.date.issued.fl_str_mv |
2011-03 |
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2012-01-06T11:55:31Z |
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2012-01-06T11:55:31Z |
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1678-8060 |
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106(2) |
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