An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens

Detalhes bibliográficos
Autor(a) principal: Teixeira, Márcia Cristina Aquino
Data de Publicação: 2011
Outros Autores: Oliveira, Geraldo Gileno de Sá, Santos, Patrícia Oliveira Meira, Bahiense, Thiago Campanharo, Silva, Virgínia Maria Goes da, Rodrigues, Márcio Silva, Larangeira, Daniela Farias, dosSantos, Washington Luis Conrado, Pontes-de-Carvalho, Lain Carlos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6049
Resumo: Universidade Federal da Bahia. Salvador, Bahia, Brasil
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spelling Teixeira, Márcia Cristina AquinoOliveira, Geraldo Gileno de SáSantos, Patrícia Oliveira MeiraBahiense, Thiago CampanharoSilva, Virgínia Maria Goes daRodrigues, Márcio SilvaLarangeira, Daniela FariasdosSantos, Washington Luis ConradoPontes-de-Carvalho, Lain Carlos2012-12-20T19:35:38Z2012-12-20T19:35:38Z2011TEIXEIRA, M. C. A. et al. An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens. Memórias do Instituto Oswaldo Cruz, v. 106, n. 2, p. 182-189, mar. 2011.0074-0276https://www.arca.fiocruz.br/handle/icict/6049engAn experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigensinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Monte Tabor. Centro Ítalo Brasileiro de Promoção Sanitária. Salvador, BA, Brasil / Universidade Federal do Ceará. Fortaleza, CE, BrasilUniversidade Federal da Bahia. Salvador, Bahia, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / 4Universidade Estadual do Sudoeste da Bahia. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, Bahia, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Publica. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil 5Escola Bahiana de Medicina e Saúde Publica. Salvador, BA, BrasilDomestic dogs are considered to be the main reservoirs of zoonotic visceral leishmaniasis. In this work, we evaluated a protocol to induce Leishmania infantum/Leishmania chagasi-specific cellular and humoral immune responses in dogs, which consisted of two injections of Leishmania promastigote lysate followed by a subcutaneous inoculation of viable promastigotes. The primary objective was to establish a canine experimental model to provide positive controls for testing immune responses to Leishmania in laboratory conditions. After inoculation of viable promastigotes, specific proliferative responses of peripheral blood mononuclear cells (PBMCs) to either Leishmania lysate or recombinant proteins, the in vitro production of interferon-γ by antigen-stimulated PBMCs and a significant increase in circulating levels of anti-Leishmania antibodies were observed. The immunized dogs also displayed positive delayed-type hypersensitivity reactions to Leishmania crude antigens and to purified recombinant proteins. An important finding that supports the suitability of the dogs as positive controls is that they remained healthy for the entire observation period, i.e., more than seven years after infection. Following the Leishmania antigen lysate injections, the infection of dogs by the subcutaneous route appears to induce a sustained cellular immune response, leading to an asymptomatic infection. This provides a useful model for both the selection of immunogenic Leishmania antigens and for immunobiological studies on their possible immunoprotective activities.Leishmania infantumLeishmania chagasiDogsImmunizationCellular immune responseAnticorpos Antiprotozoários/sangueAntígenos de Protozoários/imunologiaDoenças do Cão/imunologiaImunidade Celular/imunologiaLeishmania infantum/imunologiaLeishmaniose Visceral/veterináriaAnticorpos Antiprotozoários/imunologiaProliferação de CélulasELISAHipersensibilidade Tardia/imunologiaInterferon gama/sangueInterferon gama/imunologiaLeishmania infantumLeishmaniose Visceral/imunologiaAtivação Linfocítica/imunologiaModelos AnimaisFatores de TempoAnimaisCãesResearch Support, Non-U.S. Gov'tinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALTeixeira, Márcia Cristina Aquino et al. 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dc.title.pt_BR.fl_str_mv An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
title An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
spellingShingle An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
Teixeira, Márcia Cristina Aquino
Leishmania infantum
Leishmania chagasi
Dogs
Immunization
Cellular immune response
Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/imunologia
Doenças do Cão/imunologia
Imunidade Celular/imunologia
Leishmania infantum/imunologia
Leishmaniose Visceral/veterinária
Anticorpos Antiprotozoários/imunologia
Proliferação de Células
ELISA
Hipersensibilidade Tardia/imunologia
Interferon gama/sangue
Interferon gama/imunologia
Leishmania infantum
Leishmaniose Visceral/imunologia
Ativação Linfocítica/imunologia
Modelos Animais
Fatores de Tempo
Animais
Cães
Research Support, Non-U.S. Gov't
title_short An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
title_full An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
title_fullStr An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
title_full_unstemmed An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
title_sort An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens
author Teixeira, Márcia Cristina Aquino
author_facet Teixeira, Márcia Cristina Aquino
Oliveira, Geraldo Gileno de Sá
Santos, Patrícia Oliveira Meira
Bahiense, Thiago Campanharo
Silva, Virgínia Maria Goes da
Rodrigues, Márcio Silva
Larangeira, Daniela Farias
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
author_role author
author2 Oliveira, Geraldo Gileno de Sá
Santos, Patrícia Oliveira Meira
Bahiense, Thiago Campanharo
Silva, Virgínia Maria Goes da
Rodrigues, Márcio Silva
Larangeira, Daniela Farias
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Teixeira, Márcia Cristina Aquino
Oliveira, Geraldo Gileno de Sá
Santos, Patrícia Oliveira Meira
Bahiense, Thiago Campanharo
Silva, Virgínia Maria Goes da
Rodrigues, Márcio Silva
Larangeira, Daniela Farias
dosSantos, Washington Luis Conrado
Pontes-de-Carvalho, Lain Carlos
dc.subject.en.pt_BR.fl_str_mv Leishmania infantum
Leishmania chagasi
Dogs
Immunization
Cellular immune response
topic Leishmania infantum
Leishmania chagasi
Dogs
Immunization
Cellular immune response
Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/imunologia
Doenças do Cão/imunologia
Imunidade Celular/imunologia
Leishmania infantum/imunologia
Leishmaniose Visceral/veterinária
Anticorpos Antiprotozoários/imunologia
Proliferação de Células
ELISA
Hipersensibilidade Tardia/imunologia
Interferon gama/sangue
Interferon gama/imunologia
Leishmania infantum
Leishmaniose Visceral/imunologia
Ativação Linfocítica/imunologia
Modelos Animais
Fatores de Tempo
Animais
Cães
Research Support, Non-U.S. Gov't
dc.subject.decs.pt_BR.fl_str_mv Anticorpos Antiprotozoários/sangue
Antígenos de Protozoários/imunologia
Doenças do Cão/imunologia
Imunidade Celular/imunologia
Leishmania infantum/imunologia
Leishmaniose Visceral/veterinária
Anticorpos Antiprotozoários/imunologia
Proliferação de Células
ELISA
Hipersensibilidade Tardia/imunologia
Interferon gama/sangue
Interferon gama/imunologia
Leishmania infantum
Leishmaniose Visceral/imunologia
Ativação Linfocítica/imunologia
Modelos Animais
Fatores de Tempo
Animais
Cães
Research Support, Non-U.S. Gov't
description Universidade Federal da Bahia. Salvador, Bahia, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-12-20T19:35:38Z
dc.date.available.fl_str_mv 2012-12-20T19:35:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TEIXEIRA, M. C. A. et al. An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens. Memórias do Instituto Oswaldo Cruz, v. 106, n. 2, p. 182-189, mar. 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6049
dc.identifier.issn.none.fl_str_mv 0074-0276
identifier_str_mv TEIXEIRA, M. C. A. et al. An experimental protocol for the establishment of dogs with long-term cellular immune reactions to Leishmania antigens. Memórias do Instituto Oswaldo Cruz, v. 106, n. 2, p. 182-189, mar. 2011.
0074-0276
url https://www.arca.fiocruz.br/handle/icict/6049
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language eng
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eu_rights_str_mv openAccess
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