Detection of specific IgE antibodies in parasite diseases
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1999 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/7048 |
Resumo: | p. 1101-1105 |
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1932 |
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Atta, Maria Luiza Brito de SousaAraujo, Maria Ilma Andrade SantosD'Oliveira Junior, ArgemiroJesus, A. Ribeiro deAlmeida, Roque Pacheco deAtta, Ajax MercêsCarvalho Filho, Edgar Marcelino deAtta, Maria Luiza Brito de SousaAraujo, Maria Ilma Andrade SantosD'Oliveira Junior, ArgemiroJesus, A. Ribeiro deAlmeida, Roque Pacheco deAtta, Ajax MercêsCarvalho Filho, Edgar Marcelino de2012-10-24T18:07:09Z2012-10-24T18:07:09Z19991414-431Xhttp://www.repositorio.ufba.br/ri/handle/ri/7048v. 32, n. 9p. 1101-1105Activation of Th1 or Th2 cells is associated with production of specific immunoglobulin isotypes, offering the opportunity to use antibody measurement for evaluation of T cell function. Schistosomiasis and visceral leishmaniasis are diseases associated with Th2 activation. However, an IgE response is not always detected in these patients. In the present study we evaluated specific IgE antibodies to S. mansoni and L. chagasi antigens by ELISA after depletion of serum IgG with protein G immobilized on Sepharose beads or RF-absorbent (purified sheep IgG antibodies anti-human IgG). In schistosomiasis patients, specific IgE to SWAP antigen was demonstrable in only 10 of 21 patients (48%) (mean absorbance ± SD = 0.102 ± 0.195) when unabsorbed serum was used. Depletion of IgG with protein G increased the number of specific IgE-positive tests to 13 (62%) and the use of RF-absorbent increased the number of positive results to 20 (95%) (mean absorbances ± SD = 0.303 ± 0.455 and 0.374 ± 0.477, respectively). Specific IgE anti-L. chagasi antibodies were not detected in unabsorbed serum from visceral leishmaniasis patients. When IgG was depleted with protein G, IgE antibodies were detected in only 3 (11%) of 27 patients, and the use of RF-absorbent permitted the detection of this isotype in all 27 visceral leishmaniasis sera tested (mean absorbance ± SD = 0.104 ± 0.03). These data show that the presence of IgG antibodies may prevent the detection of a specific IgE response in these parasite diseases. RF-absorbent, a reagent that blocks IgG-binding sites and also removes rheumatoid factor, was more efficient than protein G for the demonstration of specific IgE antibodies.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2012-10-24T18:07:09Z No. of bitstreams: 1 Souza-Atta, M.L.B..pdf: 169960 bytes, checksum: 536c503cc92dd82e815a9c8339681322 (MD5)Made available in DSpace on 2012-10-24T18:07:09Z (GMT). No. of bitstreams: 1 Souza-Atta, M.L.B..pdf: 169960 bytes, checksum: 536c503cc92dd82e815a9c8339681322 (MD5) Previous issue date: 1999Ribeirão PretoBrazilian Journal of Medical and Biological Researchhttp://dx.doi.org/10.1590/S0100-879X1999000900008reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAIgE antibodiesimmunoassaysschistosomiasis mansonivisceral leishmaniasisKala-azarDetection of specific IgE antibodies in parasite diseasesBrazilian Journal of Medical and Biological Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALSouza-Atta, M.L.B..pdfSouza-Atta, M.L.B..pdfapplication/pdf169960https://repositorio.ufba.br/bitstream/ri/7048/1/Souza-Atta%2c%20M.L.B..pdf536c503cc92dd82e815a9c8339681322MD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/7048/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXTSouza-Atta, M.L.B..pdf.txtSouza-Atta, M.L.B..pdf.txtExtracted texttext/plain17727https://repositorio.ufba.br/bitstream/ri/7048/3/Souza-Atta%2c%20M.L.B..pdf.txt675e20e3e920547792a5a1a247ac2a38MD53ri/70482022-07-05 14:03:07.524oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:07Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Detection of specific IgE antibodies in parasite diseases |
| dc.title.alternative.pt_BR.fl_str_mv |
Brazilian Journal of Medical and Biological Research |
| title |
Detection of specific IgE antibodies in parasite diseases |
| spellingShingle |
Detection of specific IgE antibodies in parasite diseases Atta, Maria Luiza Brito de Sousa IgE antibodies immunoassays schistosomiasis mansoni visceral leishmaniasis Kala-azar |
| title_short |
Detection of specific IgE antibodies in parasite diseases |
| title_full |
Detection of specific IgE antibodies in parasite diseases |
| title_fullStr |
Detection of specific IgE antibodies in parasite diseases |
| title_full_unstemmed |
Detection of specific IgE antibodies in parasite diseases |
| title_sort |
Detection of specific IgE antibodies in parasite diseases |
| author |
Atta, Maria Luiza Brito de Sousa |
| author_facet |
Atta, Maria Luiza Brito de Sousa Araujo, Maria Ilma Andrade Santos D'Oliveira Junior, Argemiro Jesus, A. Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de |
| author_role |
author |
| author2 |
Araujo, Maria Ilma Andrade Santos D'Oliveira Junior, Argemiro Jesus, A. Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Atta, Maria Luiza Brito de Sousa Araujo, Maria Ilma Andrade Santos D'Oliveira Junior, Argemiro Jesus, A. Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de Atta, Maria Luiza Brito de Sousa Araujo, Maria Ilma Andrade Santos D'Oliveira Junior, Argemiro Jesus, A. Ribeiro de Almeida, Roque Pacheco de Atta, Ajax Mercês Carvalho Filho, Edgar Marcelino de |
| dc.subject.por.fl_str_mv |
IgE antibodies immunoassays schistosomiasis mansoni visceral leishmaniasis Kala-azar |
| topic |
IgE antibodies immunoassays schistosomiasis mansoni visceral leishmaniasis Kala-azar |
| description |
p. 1101-1105 |
| publishDate |
1999 |
| dc.date.issued.fl_str_mv |
1999 |
| dc.date.accessioned.fl_str_mv |
2012-10-24T18:07:09Z |
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2012-10-24T18:07:09Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/7048 |
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1414-431X |
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v. 32, n. 9 |
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1414-431X v. 32, n. 9 |
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http://www.repositorio.ufba.br/ri/handle/ri/7048 |
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Brazilian Journal of Medical and Biological Research |
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Brazilian Journal of Medical and Biological Research |
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http://dx.doi.org/10.1590/S0100-879X1999000900008 |
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