IgG anti-Ige autoantibodies in visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Atta, Ajax Mercês
Data de Publicação: 2002
Outros Autores: Atta, Maria Luiza Brito de Sousa, D'Oliveira Junior, Argemiro, Almeida, Roque Pacheco de, Araujo, Maria Ilma Andrade Santos, Carvalho Filho, Edgar Marcelino de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7598
Resumo: p. 101-103
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spelling Atta, Ajax MercêsAtta, Maria Luiza Brito de SousaD'Oliveira Junior, ArgemiroAlmeida, Roque Pacheco deAraujo, Maria Ilma Andrade SantosCarvalho Filho, Edgar Marcelino deAtta, Ajax MercêsAtta, Maria Luiza Brito de SousaD'Oliveira Junior, ArgemiroAlmeida, Roque Pacheco deAraujo, Maria Ilma Andrade SantosCarvalho Filho, Edgar Marcelino de2012-12-17T15:34:45Z2012-12-17T15:34:45Z2002-010074-0276http://www.repositorio.ufba.br/ri/handle/ri/7598v. 97, n. 1p. 101-103Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 ± 0.43) than sera from healthy individuals (OD = 1.35 ± 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 ± 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa e-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-17T15:34:45Z No. of bitstreams: 1 4291.pdf: 74717 bytes, checksum: 58e675ae0f403132ba5858e62a9b23c7 (MD5)Made available in DSpace on 2012-12-17T15:34:45Z (GMT). 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dc.title.pt_BR.fl_str_mv IgG anti-Ige autoantibodies in visceral leishmaniasis
dc.title.alternative.pt_BR.fl_str_mv Memórias do Instituto Oswaldo Cruz
title IgG anti-Ige autoantibodies in visceral leishmaniasis
spellingShingle IgG anti-Ige autoantibodies in visceral leishmaniasis
Atta, Ajax Mercês
IgE
IgG anti-IgE
protein G
visceral leishmaniasis
schistosomiasis mansoni
title_short IgG anti-Ige autoantibodies in visceral leishmaniasis
title_full IgG anti-Ige autoantibodies in visceral leishmaniasis
title_fullStr IgG anti-Ige autoantibodies in visceral leishmaniasis
title_full_unstemmed IgG anti-Ige autoantibodies in visceral leishmaniasis
title_sort IgG anti-Ige autoantibodies in visceral leishmaniasis
author Atta, Ajax Mercês
author_facet Atta, Ajax Mercês
Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
author_role author
author2 Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Atta, Ajax Mercês
Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
Atta, Ajax Mercês
Atta, Maria Luiza Brito de Sousa
D'Oliveira Junior, Argemiro
Almeida, Roque Pacheco de
Araujo, Maria Ilma Andrade Santos
Carvalho Filho, Edgar Marcelino de
dc.subject.por.fl_str_mv IgE
IgG anti-IgE
protein G
visceral leishmaniasis
schistosomiasis mansoni
topic IgE
IgG anti-IgE
protein G
visceral leishmaniasis
schistosomiasis mansoni
description p. 101-103
publishDate 2002
dc.date.issued.fl_str_mv 2002-01
dc.date.accessioned.fl_str_mv 2012-12-17T15:34:45Z
dc.date.available.fl_str_mv 2012-12-17T15:34:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7598
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.number.pt_BR.fl_str_mv v. 97, n. 1
identifier_str_mv 0074-0276
v. 97, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/7598
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0074-02762002000100017
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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