Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world

Detalhes bibliográficos
Autor(a) principal: Salay, G.
Data de Publicação: 2007
Outros Autores: Dorta, M. L., Santos, N. M., Mortara, R. A., Brodskyn, Claudia Ida, Oliveira, C. I., Barbiéri, C. L., Rodrigues, M. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14563
Resumo: Texto completo; acesso restrito. p. 1173-1181
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spelling Salay, G.Dorta, M. L.Santos, N. M.Mortara, R. A.Brodskyn, Claudia IdaOliveira, C. I.Barbiéri, C. L.Rodrigues, M. M.Salay, G.Dorta, M. L.Santos, N. M.Mortara, R. A.Brodskyn, Claudia IdaOliveira, C. I.Barbiéri, C. L.Rodrigues, M. M.2014-02-10T20:17:47Z20071556-6811http://repositorio.ufba.br/ri/handle/ri/14563v. 14, n. 9Texto completo; acesso restrito. p. 1173-1181We evaluated whether four recombinant antigens previously used for vaccination against experimental infection with Leishmania (Leishmania) major could also induce protective immunity against a challenge with Leishmania (Viannia) braziliensis, the species responsible for 90% of the 28,712 annual cases of cutaneous and mucocutaneous leishmaniasis recorded in Brazil during the year of 2004. Initially, we isolated the homolog genes encoding four L. (V.) braziliensis antigens: (i) homologue of receptor for activated C kinase, (ii) thiol-specific antioxidant, (iii) Leishmania elongation and initiation factor, and (iv) L. (L.) major stress-inducible protein 1. At the deduced amino acid level, all four open reading frames had a high degree of identity with the previously described genes of L. (L.) major being expressed on promastigotes and amastigotes of L. (V.) braziliensis. These genes were inserted into the vector pcDNA3 or expressed as bacterial recombinant proteins. After immunization with recombinant plasmids or proteins, BALB/c mice generated specific antibody or cell-mediated immune responses (gamma interferon production). After an intradermal challenge with L. (V.) braziliensis infective promastigotes, no significant reduction on the lesions was detected. We conclude that the protective immunity afforded by these four vaccine candidates against experimental cutaneous leishmaniasis caused by L. (L.) major could not be reproduced against a challenge with L. (V.) braziliensis. Although negative, we consider our results important since they suggest that studies aimed at the development of an effective vaccine against L. (V.) braziliensis, the main causative agent of cutaneous leishmaniasis in the New World, should be redirected toward distinct antigens or different vaccination strategies.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-20T15:25:56Z No. of bitstreams: 1 10.1128-CVI.00060-07.pdf: 700576 bytes, checksum: 749f6c26ef0d5f3f1b4a5a6e54072e2f (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-02-10T20:17:47Z (GMT) No. of bitstreams: 1 10.1128-CVI.00060-07.pdf: 700576 bytes, checksum: 749f6c26ef0d5f3f1b4a5a6e54072e2f (MD5)Made available in DSpace on 2014-02-10T20:17:47Z (GMT). No. of bitstreams: 1 10.1128-CVI.00060-07.pdf: 700576 bytes, checksum: 749f6c26ef0d5f3f1b4a5a6e54072e2f (MD5) Previous issue date: 2007http://dx.doi.org/10.1128/CVI.00060-07reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeishmaniaVacinas contra LeishmanioseTesting of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new worldClinical and Vaccine Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL10.1128-CVI.00060-07.pdf10.1128-CVI.00060-07.pdfapplication/pdf700576https://repositorio.ufba.br/bitstream/ri/14563/1/10.1128-CVI.00060-07.pdf749f6c26ef0d5f3f1b4a5a6e54072e2fMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14563/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14563/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT10.1128-CVI.00060-07.pdf.txt10.1128-CVI.00060-07.pdf.txtExtracted texttext/plain52510https://repositorio.ufba.br/bitstream/ri/14563/3/10.1128-CVI.00060-07.pdf.txtdb1c3e4dece060c6d3f1cf71458b9b74MD53ri/145632022-08-08 13:20:03.767oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:20:03Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
dc.title.alternative.pt_BR.fl_str_mv Clinical and Vaccine Immunology
title Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
spellingShingle Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
Salay, G.
Leishmania
Vacinas contra Leishmaniose
title_short Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
title_full Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
title_fullStr Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
title_full_unstemmed Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
title_sort Testing of four Leishmania vaccine candidates in a mouse model of infection with Leishmania (Viannia) braziliensis, the main causative agent of cutaneous leishmaniasis in the new world
author Salay, G.
author_facet Salay, G.
Dorta, M. L.
Santos, N. M.
Mortara, R. A.
Brodskyn, Claudia Ida
Oliveira, C. I.
Barbiéri, C. L.
Rodrigues, M. M.
author_role author
author2 Dorta, M. L.
Santos, N. M.
Mortara, R. A.
Brodskyn, Claudia Ida
Oliveira, C. I.
Barbiéri, C. L.
Rodrigues, M. M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Salay, G.
Dorta, M. L.
Santos, N. M.
Mortara, R. A.
Brodskyn, Claudia Ida
Oliveira, C. I.
Barbiéri, C. L.
Rodrigues, M. M.
Salay, G.
Dorta, M. L.
Santos, N. M.
Mortara, R. A.
Brodskyn, Claudia Ida
Oliveira, C. I.
Barbiéri, C. L.
Rodrigues, M. M.
dc.subject.por.fl_str_mv Leishmania
Vacinas contra Leishmaniose
topic Leishmania
Vacinas contra Leishmaniose
description Texto completo; acesso restrito. p. 1173-1181
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-02-10T20:17:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14563
dc.identifier.issn.none.fl_str_mv 1556-6811
dc.identifier.number.pt_BR.fl_str_mv v. 14, n. 9
identifier_str_mv 1556-6811
v. 14, n. 9
url http://repositorio.ufba.br/ri/handle/ri/14563
dc.language.iso.fl_str_mv eng
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