DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5305 |
Resumo: | texto completo: acesso restrito. p. 185– 190. |
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Silva, Robson Amaro Augusto daTavares, Natália MachadoCosta, Dirceu JoaquimPitombo, Maiana AlbuquerqueBarbosa, Larissa P.Fukutani, Kiyoshi FerreiraMiranda, José CarlosOliveira, Camila Indiani deValenzuela, Jesus G.Barral, Aldina Maria PradoSoto, ManuelBarral-Netto, ManoelBrodskyn, Claudia IdaSilva, Robson Amaro Augusto daTavares, Natália MachadoCosta, Dirceu JoaquimPitombo, Maiana AlbuquerqueBarbosa, Larissa P.Fukutani, Kiyoshi FerreiraMiranda, José CarlosOliveira, Camila Indiani deValenzuela, Jesus G.Barral, Aldina Maria PradoSoto, ManuelBarral-Netto, ManoelBrodskyn, Claudia Ida2012-02-06T18:09:59Z20111873-6254http://www.repositorio.ufba.br/ri/handle/ri/5305v. 120.texto completo: acesso restrito. p. 185– 190.It was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN g production in draining lymph nodes at 7, 14 and 21 days postimmunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN g/IL10 and IFN g/TGF b in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN g/IL10 and IFN g/TGF b ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmids.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-06T18:09:59Z No. of bitstreams: 1 DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.pdf: 319546 bytes, checksum: e00f74fae48c26105c01a9a13276d3f0 (MD5)Made available in DSpace on 2012-02-06T18:09:59Z (GMT). No. of bitstreams: 1 DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.pdf: 319546 bytes, checksum: e00f74fae48c26105c01a9a13276d3f0 (MD5) Previous issue date: 2011HamsterLeishmania chagasiVisceral leishmaniasisSalivaDNA plasmidsProtectionDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasisActa Tropicainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01engreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.pdfDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.pdfapplication/pdf319546https://repositorio.ufba.br/bitstream/ri/5305/1/DNA%20vaccination%20with%20KMP11%20and%20Lutzomyia%20longipalpis%20salivary%20protein%20protects%20hamsters%20against%20visceral%20leishmaniasis.pdfe00f74fae48c26105c01a9a13276d3f0MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5305/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.pdf.txtDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis.pdf.txtExtracted texttext/plain35665https://repositorio.ufba.br/bitstream/ri/5305/3/DNA%20vaccination%20with%20KMP11%20and%20Lutzomyia%20longipalpis%20salivary%20protein%20protects%20hamsters%20against%20visceral%20leishmaniasis.pdf.txt17bc9ccff7880b7612b4d9905ac4f6ccMD53ri/53052022-07-05 14:03:03.757oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:03Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| dc.title.alternative.pt_BR.fl_str_mv |
Acta Tropica |
| title |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| spellingShingle |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis Silva, Robson Amaro Augusto da Hamster Leishmania chagasi Visceral leishmaniasis Saliva DNA plasmids Protection |
| title_short |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_full |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_fullStr |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_full_unstemmed |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_sort |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| author |
Silva, Robson Amaro Augusto da |
| author_facet |
Silva, Robson Amaro Augusto da Tavares, Natália Machado Costa, Dirceu Joaquim Pitombo, Maiana Albuquerque Barbosa, Larissa P. Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G. Barral, Aldina Maria Prado Soto, Manuel Barral-Netto, Manoel Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Tavares, Natália Machado Costa, Dirceu Joaquim Pitombo, Maiana Albuquerque Barbosa, Larissa P. Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G. Barral, Aldina Maria Prado Soto, Manuel Barral-Netto, Manoel Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Robson Amaro Augusto da Tavares, Natália Machado Costa, Dirceu Joaquim Pitombo, Maiana Albuquerque Barbosa, Larissa P. Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G. Barral, Aldina Maria Prado Soto, Manuel Barral-Netto, Manoel Brodskyn, Claudia Ida Silva, Robson Amaro Augusto da Tavares, Natália Machado Costa, Dirceu Joaquim Pitombo, Maiana Albuquerque Barbosa, Larissa P. Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G. Barral, Aldina Maria Prado Soto, Manuel Barral-Netto, Manoel Brodskyn, Claudia Ida |
| dc.subject.por.fl_str_mv |
Hamster Leishmania chagasi Visceral leishmaniasis Saliva DNA plasmids Protection |
| topic |
Hamster Leishmania chagasi Visceral leishmaniasis Saliva DNA plasmids Protection |
| description |
texto completo: acesso restrito. p. 185– 190. |
| publishDate |
2011 |
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2011 |
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2012-02-06T18:09:59Z |
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info:eu-repo/semantics/publishedVersion |
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1873-6254 |
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v. 120. |
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1873-6254 v. 120. |
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