DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7306 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil / Faculdade de Medicina. Instituto de Ciências da Saúde. Salvador, BA, Brasil |
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Silva, Robson Amaro Augusto daTavares, Natalia MachadoCosta, Dirceu JoaquimPitombo, Maiana ABarbosa, LarissaFukutani, Kiyoshi FerreiraMiranda, José CarlosOliveira, Camila Indiani deValenzuela, Jesus GBarral, Aldina Maria PradoSoto, ManuelBarral Netto, ManoelBrodskyn, Claudia Ida2014-02-10T14:08:38Z2014-02-10T14:08:38Z2011SILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/730610.1016/j.actatropica.2011.08.007engElsevierDNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil / Faculdade de Medicina. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilNational Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilUniversidad Autónoma de Madrid. Departamento de Biologia Molecular. Centro de Biologia Molecular Severo Ochoa. Madrid, SpainFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilInstituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. (iii-INCT). São Paulo, SP, BrasilIt was recently shown that immunization of hamsters with DNA plasmids coding LJM19, a sand fly salivary protein, partially protected against a challenge with Leishmania chagasi, whereas immunization with KMP11 DNA plasmid, a Leishmania antigen, induced protection against L. donovani infection. In the present study, we evaluated the protective effect of immunization with both LJM19 and KMP11 DNA plasmid together. Concerning the protection against an infection by L. chagasi, immunization with DNA plasmids coding LJM19 or KMP11, as well as with both plasmids combined, induced IFN-γ production in draining lymph nodes at 7, 14 and 21 days post-immunization. Immunized hamsters challenged with L. chagasi plus Salivary Gland Sonicate (SGS) from Lutzomyia longipalpis showed an enhancement of IFN-γ/IL-10 and IFN-γ/TGF-ß in draining lymph nodes after 7 and 14 days of infection. Two and five months after challenge, immunized animals showed reduced parasite load in the liver and spleen, as well as increased IFN-γ/IL-10 and IFN-γ/TGF-ß ratios in the spleen. Furthermore, immunized animals remained with a normal hematological profile even five months after the challenge, whereas L. chagasi in unimmunized hamsters lead to a significant anemia. The protection observed with LJM19 or KMP11 DNA plasmids used alone was very similar to the protection obtained by the combination of both plasmidsHamsterLeishmania chagasiVisceral leishmaniasisSalivaDNA plasmidsProtectionProteínas de Insetos/imunologiaLeishmaniose Visceral/prevenção & controleGlicoproteínas de Membrana/imunologiaProteínas de Protozoários/imunologiaPsychodidae/imunologiaProteínas e Peptídeos Salivares/imunologiaVacinas de DNA/imunologiaAnimaisCricetinaeFemininoProteínas de Insetos/genéticaInterferon gama/secreçãoInterleucina-10/secreçãoLeishmaniose Visceral/imunologiaFígado/parasitologiaLinfonodos/imunologiaMasculinoGlicoproteínas de Membrana/genéticaMesocricetusProteínas Recombinantes/administração & dosagemBaço/imunologiaBaço/parasitologiaFator de Crescimento Transformador beta/secreçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva Robson AA da DNA vaccination with....pdfSilva Robson AA da DNA vaccination with....pdfapplication/pdf593151https://www.arca.fiocruz.br/bitstream/icict/7306/1/Silva%20Robson%20AA%20da%20DNA%20vaccination%20with....pdfd065a962f1c7d43afb10f853b27393b5MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7306/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSilva Robson AA da DNA vaccination with....pdf.txtSilva Robson AA da DNA vaccination with....pdf.txtExtracted texttext/plain36146https://www.arca.fiocruz.br/bitstream/icict/7306/5/Silva%20Robson%20AA%20da%20DNA%20vaccination%20with....pdf.txt99efa10cdff4991d5b4ef970688d21d4MD55THUMBNAILSilva Robson AA da DNA vaccination with....pdf.jpgSilva Robson AA da DNA vaccination with....pdf.jpgGenerated Thumbnailimage/jpeg2113https://www.arca.fiocruz.br/bitstream/icict/7306/4/Silva%20Robson%20AA%20da%20DNA%20vaccination%20with....pdf.jpg22be7c9ebb485fa2664cc4ba3f9fbfefMD54icict/73062023-03-15 14:33:15.932oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| spellingShingle |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis Silva, Robson Amaro Augusto da Hamster Leishmania chagasi Visceral leishmaniasis Saliva DNA plasmids Protection Proteínas de Insetos/imunologia Leishmaniose Visceral/prevenção & controle Glicoproteínas de Membrana/imunologia Proteínas de Protozoários/imunologia Psychodidae/imunologia Proteínas e Peptídeos Salivares/imunologia Vacinas de DNA/imunologia Animais Cricetinae Feminino Proteínas de Insetos/genética Interferon gama/secreção Interleucina-10/secreção Leishmaniose Visceral/imunologia Fígado/parasitologia Linfonodos/imunologia Masculino Glicoproteínas de Membrana/genética Mesocricetus Proteínas Recombinantes/administração & dosagem Baço/imunologia Baço/parasitologia Fator de Crescimento Transformador beta/secreção |
| title_short |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_full |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_fullStr |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_full_unstemmed |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| title_sort |
DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis |
| author |
Silva, Robson Amaro Augusto da |
| author_facet |
Silva, Robson Amaro Augusto da Tavares, Natalia Machado Costa, Dirceu Joaquim Pitombo, Maiana A Barbosa, Larissa Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G Barral, Aldina Maria Prado Soto, Manuel Barral Netto, Manoel Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Tavares, Natalia Machado Costa, Dirceu Joaquim Pitombo, Maiana A Barbosa, Larissa Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G Barral, Aldina Maria Prado Soto, Manuel Barral Netto, Manoel Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Robson Amaro Augusto da Tavares, Natalia Machado Costa, Dirceu Joaquim Pitombo, Maiana A Barbosa, Larissa Fukutani, Kiyoshi Ferreira Miranda, José Carlos Oliveira, Camila Indiani de Valenzuela, Jesus G Barral, Aldina Maria Prado Soto, Manuel Barral Netto, Manoel Brodskyn, Claudia Ida |
| dc.subject.en.pt_BR.fl_str_mv |
Hamster Leishmania chagasi Visceral leishmaniasis Saliva DNA plasmids Protection |
| topic |
Hamster Leishmania chagasi Visceral leishmaniasis Saliva DNA plasmids Protection Proteínas de Insetos/imunologia Leishmaniose Visceral/prevenção & controle Glicoproteínas de Membrana/imunologia Proteínas de Protozoários/imunologia Psychodidae/imunologia Proteínas e Peptídeos Salivares/imunologia Vacinas de DNA/imunologia Animais Cricetinae Feminino Proteínas de Insetos/genética Interferon gama/secreção Interleucina-10/secreção Leishmaniose Visceral/imunologia Fígado/parasitologia Linfonodos/imunologia Masculino Glicoproteínas de Membrana/genética Mesocricetus Proteínas Recombinantes/administração & dosagem Baço/imunologia Baço/parasitologia Fator de Crescimento Transformador beta/secreção |
| dc.subject.decs.pt_BR.fl_str_mv |
Proteínas de Insetos/imunologia Leishmaniose Visceral/prevenção & controle Glicoproteínas de Membrana/imunologia Proteínas de Protozoários/imunologia Psychodidae/imunologia Proteínas e Peptídeos Salivares/imunologia Vacinas de DNA/imunologia Animais Cricetinae Feminino Proteínas de Insetos/genética Interferon gama/secreção Interleucina-10/secreção Leishmaniose Visceral/imunologia Fígado/parasitologia Linfonodos/imunologia Masculino Glicoproteínas de Membrana/genética Mesocricetus Proteínas Recombinantes/administração & dosagem Baço/imunologia Baço/parasitologia Fator de Crescimento Transformador beta/secreção |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto Multidisciplinar em Saúde. Vitória da Conquista, BA, Brasil / Faculdade de Medicina. Instituto de Ciências da Saúde. Salvador, BA, Brasil |
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2011 |
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2011 |
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2014-02-10T14:08:38Z |
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2014-02-10T14:08:38Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011. |
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https://www.arca.fiocruz.br/handle/icict/7306 |
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0001-706X |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.actatropica.2011.08.007 |
| identifier_str_mv |
SILVA, R. R. A. da et al. DNA vaccination with KMP11 and Lutzomyia longipalpis salivary protein protects hamsters against visceral leishmaniasis. Acta Tropica, v. 120, n. 3, p. 185-190, 2011. 0001-706X 10.1016/j.actatropica.2011.08.007 |
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