Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12857 |
Resumo: | Texto completo: acesso restrito. p.530–541 |
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Spohr, Tânia Cristina Leite de Sampaio eStipursky, JoiceSasaki, Adriana CamposBarbosa, Pedro RochaMartins, VanessaBenjamim, Cláudia FariasRoque, Nídia FrancaCosta, Silvia LimaGomes, Flávia Carvalho AlcantaraSpohr, Tânia Cristina Leite de Sampaio eStipursky, JoiceSasaki, Adriana CamposBarbosa, Pedro RochaMartins, VanessaBenjamim, Cláudia FariasRoque, Nídia FrancaCosta, Silvia LimaGomes, Flávia Carvalho Alcantara2013-09-06T15:22:40Z20100360-4012http://www.repositorio.ufba.br/ri/handle/ri/12857v. 88, n. 3Texto completo: acesso restrito. p.530–541Neurodegenerative diseases are a major constraint on the social and economic development of many countries. Evidence has suggested that phytochemicals have an impact on brain pathology; however, both their mechanisms of action and their cell targets are incompletely known. Here, we investigated the effects of the flavonoid casticin, extracted from Croton betulaster, a common plant in the state of Bahia in Brazil, on rat cerebral cortex neurons in vitro. Treatment of neural progenitors with 10 μM casticin increased the neuronal population positive for the neuronal marker β-tubulin III and the neuronal transcriptional factor Tbr2 by approximately 20%. This event was followed by a 50% decrease in neuronal death. Pools of astrocyte (GFAP and S100β), neural (nestin), and oligodendrocyte (Olig2 and NG2) progenitors were not affected by casticin. Neither neuronal commitment nor proliferation of progenitors was affected by casticin, suggesting a neuroprotective effect of this compound. Culture of neural progenitors on casticin-treated astrocyte monolayers increased the neuronal population by 40%. This effect was reproduced by conditioned medium derived from casticin-treated astrocytes, suggesting the involvement of a soluble factor. ELISA assays of the conditioned medium revealed a 20% increase in interleukin-6 level in response to casticin. In contrast to the direct effect, neuronal death was unaffected, but a 52% decrease in the death of nestin-positive progenitors was observed. Together our data suggest that casticin influences the neuronal population by two mechanisms: 1) directly, by decreasing neuronal death, and 2) indirectly, via astrocytes, by modulating the pool of neuronal progenitors. © 2009 Wiley-Liss, Inc.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-05T14:59:26Z No. of bitstreams: 1 10.1002jnr.22218.pdf: 863061 bytes, checksum: 26dae6349dba39c894b9be4396add705 (MD5)Approved for entry into archive by Patricia Barroso(pbarroso@ufba.br) on 2013-09-06T15:22:39Z (GMT) No. of bitstreams: 1 10.1002jnr.22218.pdf: 863061 bytes, checksum: 26dae6349dba39c894b9be4396add705 (MD5)Made available in DSpace on 2013-09-06T15:22:40Z (GMT). No. of bitstreams: 1 10.1002jnr.22218.pdf: 863061 bytes, checksum: 26dae6349dba39c894b9be4396add705 (MD5) Previous issue date: 2010http://dx.doi.org/10.1002/jnr.22218reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBANeuron–glia interactionFlavonoidsAstrocyteNeurodegenerative diseasesNeurogenesisEffects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytesJournal of Neuroscience Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL10.1002jnr.22218.pdf10.1002jnr.22218.pdfapplication/pdf863061https://repositorio.ufba.br/bitstream/ri/12857/1/10.1002jnr.22218.pdf26dae6349dba39c894b9be4396add705MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12857/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT10.1002jnr.22218.pdf.txt10.1002jnr.22218.pdf.txtExtracted texttext/plain56421https://repositorio.ufba.br/bitstream/ri/12857/3/10.1002jnr.22218.pdf.txt3de230f744e40917674b2ad75483cd85MD53ri/128572022-07-05 14:03:03.559oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:03Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Neuroscience Research |
| title |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| spellingShingle |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes Spohr, Tânia Cristina Leite de Sampaio e Neuron–glia interaction Flavonoids Astrocyte Neurodegenerative diseases Neurogenesis |
| title_short |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| title_full |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| title_fullStr |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| title_full_unstemmed |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| title_sort |
Effects of the flavonoid casticin from Brazilian Croton betulaster in cerebral cortical progenitors in vitro: direct and indirect action through astrocytes |
| author |
Spohr, Tânia Cristina Leite de Sampaio e |
| author_facet |
Spohr, Tânia Cristina Leite de Sampaio e Stipursky, Joice Sasaki, Adriana Campos Barbosa, Pedro Rocha Martins, Vanessa Benjamim, Cláudia Farias Roque, Nídia Franca Costa, Silvia Lima Gomes, Flávia Carvalho Alcantara |
| author_role |
author |
| author2 |
Stipursky, Joice Sasaki, Adriana Campos Barbosa, Pedro Rocha Martins, Vanessa Benjamim, Cláudia Farias Roque, Nídia Franca Costa, Silvia Lima Gomes, Flávia Carvalho Alcantara |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Spohr, Tânia Cristina Leite de Sampaio e Stipursky, Joice Sasaki, Adriana Campos Barbosa, Pedro Rocha Martins, Vanessa Benjamim, Cláudia Farias Roque, Nídia Franca Costa, Silvia Lima Gomes, Flávia Carvalho Alcantara Spohr, Tânia Cristina Leite de Sampaio e Stipursky, Joice Sasaki, Adriana Campos Barbosa, Pedro Rocha Martins, Vanessa Benjamim, Cláudia Farias Roque, Nídia Franca Costa, Silvia Lima Gomes, Flávia Carvalho Alcantara |
| dc.subject.por.fl_str_mv |
Neuron–glia interaction Flavonoids Astrocyte Neurodegenerative diseases Neurogenesis |
| topic |
Neuron–glia interaction Flavonoids Astrocyte Neurodegenerative diseases Neurogenesis |
| description |
Texto completo: acesso restrito. p.530–541 |
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2010 |
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2010 |
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2013-09-06T15:22:40Z |
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0360-4012 |
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v. 88, n. 3 |
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0360-4012 v. 88, n. 3 |
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