Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses

Detalhes bibliográficos
Autor(a) principal: Gama, Bianca Ervatti
Data de Publicação: 2003
Outros Autores: Couceiro, José Nelson dos Santos Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/20325
Resumo: Artigo original(p.170-175)
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spelling Gama, Bianca ErvattiCouceiro, José Nelson dos Santos Silva2016-09-13T13:50:35Z2016-09-13T13:50:35Z2003-07GAMA, B. E.; COUCEIRO, J. N. dos S. Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses. R. Ci. méd. biol., Salvador, v. 2, n. 2, p. 170-175, jul./dez. 2003.2236-5222http://repositorio.ufba.br/ri/handle/ri/20325v.2, n.2Artigo original(p.170-175)Since the most ancient times, influenza viruses have caused lethal respiratory diseases. Their replication process occurs at the epithelial cells of the respiratory tract, where the surface-anchored glycoproteins hemagglutinin (HA) and hemagglutinin-esterase-fusion protein (HEF) of influenza A and C viruses respectively are responsible for the fusion process. The adsorption, sialidase and esterase activities developed by these same structures are inborn, while the fusion process is dependent of previous glycosylation and protein cleavage. Indeed, the glycosylation is strictly related to antigenicity and stability of fusion proteins. This work was designed to analyse the influence of the de-glycosylation for the development of fusion activity, using influenza A and C viruses as study models. The de-glycosylation provoked significative reduction in the fusogenic activity, inducing a reduction equal to 51.0%, 87.5%, 95.5% and 79.3% for A/Memphis/102/72, A/FM/1/47, C/Taylor/1233/47 and C/Paris/1/67 respectively. However, this activity was improved at certain pH values, 10.1% (pH 5.8), 59.4% (pH 5.8), 32.5% (pH 5.8) and 80.7% (pH 5.4) for the 95.7% at pH 5.2 for A/Memphis/102/72, A/FM/1/47, C/Taylor/1233/47 and C/Paris/1/67 of influenza viruses respectively. The fusogenic activity of certain samples was also improved at some pH values. These results permit to conclude that the level of glycosylation is closely related to the protein stability and the de-glycosylation process causes a significative influence on the fusion biological activity.Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-13T13:50:35Z No. of bitstreams: 1 R. Ci. méd. biol., v.2, n. 2-2003.pdf: 250796 bytes, checksum: 0e7df8df43b203405a14cb935bf99813 (MD5)Made available in DSpace on 2016-09-13T13:50:35Z (GMT). No. of bitstreams: 1 R. Ci. méd. biol., v.2, n. 2-2003.pdf: 250796 bytes, checksum: 0e7df8df43b203405a14cb935bf99813 (MD5) Previous issue date: 2003-07SalvadorInstituto de Ciências da Saúde/ Universidade Federal da Bahiahttp://www.portalseer.ufba.br/index.php/cmbio/issue/view/497reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAInfluenza virusesHemagglutinin (HA)Hemagglutinin-esterase-fusion protein (HEF)Fusion activityGlycosylationGlycosylation influencing on fusion activity of HA and HEF structures of influenza virusesRevista de Ciências Médicas e Biológicasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessporORIGINALR. Ci. méd. biol., v.2, n. 2-2003.pdfR. 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dc.title.pt_BR.fl_str_mv Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
dc.title.alternative.pt_BR.fl_str_mv Revista de Ciências Médicas e Biológicas
title Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
spellingShingle Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
Gama, Bianca Ervatti
Influenza viruses
Hemagglutinin (HA)
Hemagglutinin-esterase-fusion protein (HEF)
Fusion activity
Glycosylation
title_short Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
title_full Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
title_fullStr Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
title_full_unstemmed Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
title_sort Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses
author Gama, Bianca Ervatti
author_facet Gama, Bianca Ervatti
Couceiro, José Nelson dos Santos Silva
author_role author
author2 Couceiro, José Nelson dos Santos Silva
author2_role author
dc.contributor.author.fl_str_mv Gama, Bianca Ervatti
Couceiro, José Nelson dos Santos Silva
dc.subject.por.fl_str_mv Influenza viruses
Hemagglutinin (HA)
Hemagglutinin-esterase-fusion protein (HEF)
Fusion activity
Glycosylation
topic Influenza viruses
Hemagglutinin (HA)
Hemagglutinin-esterase-fusion protein (HEF)
Fusion activity
Glycosylation
description Artigo original(p.170-175)
publishDate 2003
dc.date.issued.fl_str_mv 2003-07
dc.date.accessioned.fl_str_mv 2016-09-13T13:50:35Z
dc.date.available.fl_str_mv 2016-09-13T13:50:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv GAMA, B. E.; COUCEIRO, J. N. dos S. Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses. R. Ci. méd. biol., Salvador, v. 2, n. 2, p. 170-175, jul./dez. 2003.
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/20325
dc.identifier.issn.none.fl_str_mv 2236-5222
dc.identifier.number.pt_BR.fl_str_mv v.2, n.2
identifier_str_mv GAMA, B. E.; COUCEIRO, J. N. dos S. Glycosylation influencing on fusion activity of HA and HEF structures of influenza viruses. R. Ci. méd. biol., Salvador, v. 2, n. 2, p. 170-175, jul./dez. 2003.
2236-5222
v.2, n.2
url http://repositorio.ufba.br/ri/handle/ri/20325
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
dc.source.pt_BR.fl_str_mv http://www.portalseer.ufba.br/index.php/cmbio/issue/view/497
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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