A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence

Detalhes bibliográficos
Autor(a) principal: Leite, Oldair Donizeti
Data de Publicação: 2010
Outros Autores: Vieira, Heberth J., Fatibello-Filho, Orlando, Rocha, Fábio Rodrigo Piovezani
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/4952
Resumo: p. 1710-1717.
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spelling Leite, Oldair DonizetiVieira, Heberth J.Fatibello-Filho, OrlandoRocha, Fábio Rodrigo PiovezaniLeite, Oldair DonizetiVieira, Heberth J.Fatibello-Filho, OrlandoRocha, Fábio Rodrigo Piovezani2011-12-21T11:47:11Z2011-12-21T11:47:11Z20100103-5053http://www.repositorio.ufba.br/ri/handle/ri/4952v. 21, n. 9.p. 1710-1717.Um sistema de análises em fluxo com multicomutação com detecção por quimiluminescência foi desenvolvido para a determinação de colesterol livre e total sem alterações no módulo de análises. Colesterol estearase e colesterol oxidase foram imobilizadas em esferas de vidro via glutaraldeído/3-aminopropiletoxisilano e mini-colunas contendo as enzimas foram usadas para tratamento das amostras em linha. Ésteres de colesterol foram clivados a colesterol e ácidos graxos na coluna empacotada com colesterol estearase. No reator contendo colesterol oxidase, o analito foi convertido a 4-colesten-3-ona e peróxido de hidrogênio. A detecção foi baseada na quimiluminescência produzida por H2O2 no sistema hexacianoferrato(III)-luminol. Os efeitos de variáveis químicas e hidrodinâmicas sobre o sinal de quimiluminescência foram avaliados. As curvas analíticas foram lineares entre 250 e 2500 mg L-1 e 500 e 4000 mg L-1, para colesterol livre e total, respectivamente. Limites de detecção para ambos os analitos foram estimados em 60 mg L-1 (99,7% de confiança). A frequência de amostragem foi de 55 h-1 e o consumo de reagentes foi de 350 µg de luminol e 2,6 mg de hexacianoferrato(III) de potássio por determinação. O procedimento desenvolvido foi aplicado à determinação de colesterol em ovos e soro sanguíneo humano gerando resultados concordantes com o procedimento espectrofotométrico de referência com nível de confiança de 95%.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2011-12-21T11:47:10Z No. of bitstreams: 1 v21n9a17.pdf: 521237 bytes, checksum: d611778389e4cc1f8e273e711fdf02ee (MD5)Made available in DSpace on 2011-12-21T11:47:11Z (GMT). No. of bitstreams: 1 v21n9a17.pdf: 521237 bytes, checksum: d611778389e4cc1f8e273e711fdf02ee (MD5) Previous issue date: 2010São Paulohttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000900017&lng=en&nrm=isoreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAFlow analysisMulticommutationChemiluminescenceCholesterolCholesterol esteraseCholesterolOxidaseA multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescenceJournal of the Brazilian Chemical Societyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALv21n9a17.pdfv21n9a17.pdfapplication/pdf521237https://repositorio.ufba.br/bitstream/ri/4952/1/v21n9a17.pdfd611778389e4cc1f8e273e711fdf02eeMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/4952/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXTv21n9a17.pdf.txtv21n9a17.pdf.txtExtracted texttext/plain31287https://repositorio.ufba.br/bitstream/ri/4952/3/v21n9a17.pdf.txtdff35b4fb629f96a1e598c3887f6b1feMD53ri/49522022-07-05 14:03:18.052oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:18Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
dc.title.alternative.pt_BR.fl_str_mv Journal of the Brazilian Chemical Society
title A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
spellingShingle A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
Leite, Oldair Donizeti
Flow analysis
Multicommutation
Chemiluminescence
Cholesterol
Cholesterol esterase
Cholesterol
Oxidase
title_short A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
title_full A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
title_fullStr A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
title_full_unstemmed A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
title_sort A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
author Leite, Oldair Donizeti
author_facet Leite, Oldair Donizeti
Vieira, Heberth J.
Fatibello-Filho, Orlando
Rocha, Fábio Rodrigo Piovezani
author_role author
author2 Vieira, Heberth J.
Fatibello-Filho, Orlando
Rocha, Fábio Rodrigo Piovezani
author2_role author
author
author
dc.contributor.author.fl_str_mv Leite, Oldair Donizeti
Vieira, Heberth J.
Fatibello-Filho, Orlando
Rocha, Fábio Rodrigo Piovezani
Leite, Oldair Donizeti
Vieira, Heberth J.
Fatibello-Filho, Orlando
Rocha, Fábio Rodrigo Piovezani
dc.subject.por.fl_str_mv Flow analysis
Multicommutation
Chemiluminescence
Cholesterol
Cholesterol esterase
Cholesterol
Oxidase
topic Flow analysis
Multicommutation
Chemiluminescence
Cholesterol
Cholesterol esterase
Cholesterol
Oxidase
description p. 1710-1717.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2011-12-21T11:47:11Z
dc.date.available.fl_str_mv 2011-12-21T11:47:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/4952
dc.identifier.issn.none.fl_str_mv 0103-5053
dc.identifier.number.pt_BR.fl_str_mv v. 21, n. 9.
identifier_str_mv 0103-5053
v. 21, n. 9.
url http://www.repositorio.ufba.br/ri/handle/ri/4952
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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