A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4952 |
Resumo: | p. 1710-1717. |
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Leite, Oldair DonizetiVieira, Heberth J.Fatibello-Filho, OrlandoRocha, Fábio Rodrigo PiovezaniLeite, Oldair DonizetiVieira, Heberth J.Fatibello-Filho, OrlandoRocha, Fábio Rodrigo Piovezani2011-12-21T11:47:11Z2011-12-21T11:47:11Z20100103-5053http://www.repositorio.ufba.br/ri/handle/ri/4952v. 21, n. 9.p. 1710-1717.Um sistema de análises em fluxo com multicomutação com detecção por quimiluminescência foi desenvolvido para a determinação de colesterol livre e total sem alterações no módulo de análises. Colesterol estearase e colesterol oxidase foram imobilizadas em esferas de vidro via glutaraldeído/3-aminopropiletoxisilano e mini-colunas contendo as enzimas foram usadas para tratamento das amostras em linha. Ésteres de colesterol foram clivados a colesterol e ácidos graxos na coluna empacotada com colesterol estearase. No reator contendo colesterol oxidase, o analito foi convertido a 4-colesten-3-ona e peróxido de hidrogênio. A detecção foi baseada na quimiluminescência produzida por H2O2 no sistema hexacianoferrato(III)-luminol. Os efeitos de variáveis químicas e hidrodinâmicas sobre o sinal de quimiluminescência foram avaliados. As curvas analíticas foram lineares entre 250 e 2500 mg L-1 e 500 e 4000 mg L-1, para colesterol livre e total, respectivamente. Limites de detecção para ambos os analitos foram estimados em 60 mg L-1 (99,7% de confiança). A frequência de amostragem foi de 55 h-1 e o consumo de reagentes foi de 350 µg de luminol e 2,6 mg de hexacianoferrato(III) de potássio por determinação. O procedimento desenvolvido foi aplicado à determinação de colesterol em ovos e soro sanguíneo humano gerando resultados concordantes com o procedimento espectrofotométrico de referência com nível de confiança de 95%.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2011-12-21T11:47:10Z No. of bitstreams: 1 v21n9a17.pdf: 521237 bytes, checksum: d611778389e4cc1f8e273e711fdf02ee (MD5)Made available in DSpace on 2011-12-21T11:47:11Z (GMT). No. of bitstreams: 1 v21n9a17.pdf: 521237 bytes, checksum: d611778389e4cc1f8e273e711fdf02ee (MD5) Previous issue date: 2010São Paulohttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000900017&lng=en&nrm=isoreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAFlow analysisMulticommutationChemiluminescenceCholesterolCholesterol esteraseCholesterolOxidaseA multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescenceJournal of the Brazilian Chemical Societyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALv21n9a17.pdfv21n9a17.pdfapplication/pdf521237https://repositorio.ufba.br/bitstream/ri/4952/1/v21n9a17.pdfd611778389e4cc1f8e273e711fdf02eeMD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/4952/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXTv21n9a17.pdf.txtv21n9a17.pdf.txtExtracted texttext/plain31287https://repositorio.ufba.br/bitstream/ri/4952/3/v21n9a17.pdf.txtdff35b4fb629f96a1e598c3887f6b1feMD53ri/49522022-07-05 14:03:18.052oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:18Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
dc.title.pt_BR.fl_str_mv |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
dc.title.alternative.pt_BR.fl_str_mv |
Journal of the Brazilian Chemical Society |
title |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
spellingShingle |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence Leite, Oldair Donizeti Flow analysis Multicommutation Chemiluminescence Cholesterol Cholesterol esterase Cholesterol Oxidase |
title_short |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
title_full |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
title_fullStr |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
title_full_unstemmed |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
title_sort |
A multicommuted flow procedure for the determination of total and free cholesterol in eggs and human blood serum by chemiluminescence |
author |
Leite, Oldair Donizeti |
author_facet |
Leite, Oldair Donizeti Vieira, Heberth J. Fatibello-Filho, Orlando Rocha, Fábio Rodrigo Piovezani |
author_role |
author |
author2 |
Vieira, Heberth J. Fatibello-Filho, Orlando Rocha, Fábio Rodrigo Piovezani |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Leite, Oldair Donizeti Vieira, Heberth J. Fatibello-Filho, Orlando Rocha, Fábio Rodrigo Piovezani Leite, Oldair Donizeti Vieira, Heberth J. Fatibello-Filho, Orlando Rocha, Fábio Rodrigo Piovezani |
dc.subject.por.fl_str_mv |
Flow analysis Multicommutation Chemiluminescence Cholesterol Cholesterol esterase Cholesterol Oxidase |
topic |
Flow analysis Multicommutation Chemiluminescence Cholesterol Cholesterol esterase Cholesterol Oxidase |
description |
p. 1710-1717. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2011-12-21T11:47:11Z |
dc.date.available.fl_str_mv |
2011-12-21T11:47:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.repositorio.ufba.br/ri/handle/ri/4952 |
dc.identifier.issn.none.fl_str_mv |
0103-5053 |
dc.identifier.number.pt_BR.fl_str_mv |
v. 21, n. 9. |
identifier_str_mv |
0103-5053 v. 21, n. 9. |
url |
http://www.repositorio.ufba.br/ri/handle/ri/4952 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-50532010000900017&lng=en&nrm=iso |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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Universidade Federal da Bahia (UFBA) |
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UFBA |
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UFBA |
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Repositório Institucional da UFBA |
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Repositório Institucional da UFBA |
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