Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile

Detalhes bibliográficos
Autor(a) principal: Reis, E. A. G.
Data de Publicação: 2008
Outros Autores: Carmo, Theomira Mauadie Azevedo, Athanazio, Rodrigo Abensur, Reis, M. G., Harn Jr, D. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6087
Resumo: Acesso restrito: Texto completo. p. 169-176
id UFBA-2_af2c660728b9ca91ddb12280f52e7c00
oai_identifier_str oai:repositorio.ufba.br:ri/6087
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Reis, E. A. G.Carmo, Theomira Mauadie AzevedoAthanazio, Rodrigo AbensurReis, M. G.Harn Jr, D. A.Reis, E. A. G.Carmo, Theomira Mauadie AzevedoAthanazio, Rodrigo AbensurReis, M. G.Harn Jr, D. A.2012-06-06T18:33:24Z20080300-9475http://www.repositorio.ufba.br/ri/handle/ri/6087v. 68, n. 2Acesso restrito: Texto completo. p. 169-176We evaluated the ability of naïve monocyte-derived dendritic cells (DC) to sensitize autologous peripheral blood mononuclear cells (PBMC) to the schistosome vaccine candidate MAP4 using a priming in vitro (PIV) assay. MAP4 is a multiple antigen peptide containing B- and T-cell epitopes derived from the glycolytic enzyme triose phosphate isomerase. PBMC primed and restimulated with MAP4 first and secondary recalls (MAP4 PIV cells) were examined for cell phenotype and cytokine production. We found that after the first recall stimulation with MAP4, the major cell population was predominantly CD4+ T-cell subsets (68.5%), CD8+high (16%) and CD19+ (10%). Additionally, MAP4 PIV cells significantly expressed CD4+-HLA-DR+, -CD54+, -CD45RO+ (P < 0.0001) and -CD25+ (P < 0.0004) together with significant expression of CD80+ on CD19+ B cells (P < 0.007). Cytokine production from activated MAP4 PIV cells was predominantly Th1-like, consisting mainly of IFN-γ. Interestingly, IFN-γ production was suppressed when Schistosoma mansoni-soluble egg antigen (SEA) was added to a MAP4 PIV cell culture. Furthermore, addition of MAP4 to a SEA PIV cell culture significantly reduced secretion of IL-10. The present findings add to the knowledge gained from studies in the mouse model, and our results show that naïve donor DC, sensitized with MAP4, were able to prime and clonally expand MAP4-specific T cells towards a Th1-type response.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-06-06T18:33:24Z No. of bitstreams: 1 __onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf: 255099 bytes, checksum: 9db9d0414043f04c135a1df520808854 (MD5)Made available in DSpace on 2012-06-06T18:33:24Z (GMT). No. of bitstreams: 1 __onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf: 255099 bytes, checksum: 9db9d0414043f04c135a1df520808854 (MD5) Previous issue date: 2008http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2008.02131.x/pdfreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBASchistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine ProfileScandinavian Journal of Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdfapplication/pdf255099https://repositorio.ufba.br/bitstream/ri/6087/1/__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf9db9d0414043f04c135a1df520808854MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6087/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf.txt__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf.txtExtracted texttext/plain35994https://repositorio.ufba.br/bitstream/ri/6087/3/__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf.txt6d78f758dc5aaf094ab80e3cf1d95a50MD53ri/60872022-07-05 14:03:51.839oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:51Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
dc.title.alternative.pt_BR.fl_str_mv Scandinavian Journal of Immunology
title Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
spellingShingle Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
Reis, E. A. G.
title_short Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
title_full Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
title_fullStr Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
title_full_unstemmed Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
title_sort Schistosoma mansoni Triose Phosphate Isomerase Peptide MAP4 is Able to Trigger Naı¨ve Donor Immune Response Towards a Type-1 Cytokine Profile
author Reis, E. A. G.
author_facet Reis, E. A. G.
Carmo, Theomira Mauadie Azevedo
Athanazio, Rodrigo Abensur
Reis, M. G.
Harn Jr, D. A.
author_role author
author2 Carmo, Theomira Mauadie Azevedo
Athanazio, Rodrigo Abensur
Reis, M. G.
Harn Jr, D. A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reis, E. A. G.
Carmo, Theomira Mauadie Azevedo
Athanazio, Rodrigo Abensur
Reis, M. G.
Harn Jr, D. A.
Reis, E. A. G.
Carmo, Theomira Mauadie Azevedo
Athanazio, Rodrigo Abensur
Reis, M. G.
Harn Jr, D. A.
description Acesso restrito: Texto completo. p. 169-176
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2012-06-06T18:33:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6087
dc.identifier.issn.none.fl_str_mv 0300-9475
dc.identifier.number.pt_BR.fl_str_mv v. 68, n. 2
identifier_str_mv 0300-9475
v. 68, n. 2
url http://www.repositorio.ufba.br/ri/handle/ri/6087
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://onlinelibrary.wiley.com/doi/10.1111/j.1365-3083.2008.02131.x/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/6087/1/__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf
https://repositorio.ufba.br/bitstream/ri/6087/2/license.txt
https://repositorio.ufba.br/bitstream/ri/6087/3/__onlinelibrary.wiley.co...067846220482bd2874cd2b3d7abbf.pdf.txt
bitstream.checksum.fl_str_mv 9db9d0414043f04c135a1df520808854
1b89a9a0548218172d7c829f87a0eab9
6d78f758dc5aaf094ab80e3cf1d95a50
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459400469282816

Registros relacionados