Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.

Detalhes bibliográficos
Autor(a) principal: Reis, Eliana Almeida Gomes
Data de Publicação: 2008
Outros Autores: Carmo, Theomira Mauadie de Azevedo, Athanazio, Rodrigo, Reis, Mitermayer Galvão dos, Harn Junior, Donald A
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7530
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Reis, Eliana Almeida GomesCarmo, Theomira Mauadie de AzevedoAthanazio, RodrigoReis, Mitermayer Galvão dosHarn Junior, Donald A2014-04-24T11:49:58Z2014-04-24T11:49:58Z2008REIS, E. A. G. et al. Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile. Scandinavian Journal of Immunology, v. 68, n. 2, p. 169-176, 2008.1365-3083https://www.arca.fiocruz.br/handle/icict/753010.1111/j.1365-3083.2008.02131.xengBlackwell Publishing LtdSchistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilHarvard School of Public Health. Department of Immunology and Infectious Diseases. Boston, MA, USAWe evaluated the ability of naïve monocyte-derived dendritic cells (DC) to sensitize autologous peripheral blood mononuclear cells (PBMC) to the schistosome vaccine candidate MAP4 using a priming in vitro (PIV) assay. MAP4 is a multiple antigen peptide containing B- and T-cell epitopes derived from the glycolytic enzyme triose phosphate isomerase. PBMC primed and restimulated with MAP4 first and secondary recalls (MAP4 PIV cells) were examined for cell phenotype and cytokine production. We found that after the first recall stimulation with MAP4, the major cell population was predominantly CD4(+) T-cell subsets (68.5%), CD8(+high) (16%) and CD19(+) (10%). Additionally, MAP4 PIV cells significantly expressed CD4(+)-HLA-DR(+), -CD54(+), -CD45RO(+) (P < 0.0001) and -CD25(+) (P < 0.0004) together with significant expression of CD80(+) on CD19(+) B cells (P < 0.007). Cytokine production from activated MAP4 PIV cells was predominantly Th1-like, consisting mainly of IFN-gamma. Interestingly, IFN-gamma production was suppressed when Schistosoma mansoni-soluble egg antigen (SEA) was added to a MAP4 PIV cell culture. Furthermore, addition of MAP4 to a SEA PIV cell culture significantly reduced secretion of IL-10. The present findings add to the knowledge gained from studies in the mouse model, and our results show that naïve donor DC, sensitized with MAP4, were able to prime and clonally expand MAP4-specific T cells towards a Th1-type response.Antígenos de Helmintos/imunologiaCitocinas/imunologiaSchistosoma mansoni/imunologiaSubpopulações de Linfócitos T/imunologiaCélulas Th1/imunologiaTriose-Fosfato Isomerase/imunologiaAnimaisCitocinas/biossínteseCélulas Dendríticas/imunologiaEnsaio de Imunoadsorção EnzimáticaCitometria de FluxoHumanosAtivação Linfocitária/imunologiaPeptídeos/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALReis EA Schistosoma mansoni....pdfReis EA Schistosoma mansoni....pdfapplication/pdf326165https://www.arca.fiocruz.br/bitstream/icict/7530/1/Reis%20EA%20Schistosoma%20mansoni....pdf8827f2d6171ac6e455a7120a9bab6e84MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7530/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTReis EA Schistosoma mansoni....pdf.txtReis EA Schistosoma mansoni....pdf.txtExtracted texttext/plain36232https://www.arca.fiocruz.br/bitstream/icict/7530/5/Reis%20EA%20Schistosoma%20mansoni....pdf.txtcccd49778839f4d476f78235450b1193MD55THUMBNAILReis EA Schistosoma mansoni....pdf.jpgReis EA Schistosoma mansoni....pdf.jpgGenerated Thumbnailimage/jpeg2335https://www.arca.fiocruz.br/bitstream/icict/7530/4/Reis%20EA%20Schistosoma%20mansoni....pdf.jpg82cc8b977faf5e2e7f09e5f8d1e654a7MD54icict/75302023-03-15 14:33:54.989oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
title Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
spellingShingle Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
Reis, Eliana Almeida Gomes
Antígenos de Helmintos/imunologia
Citocinas/imunologia
Schistosoma mansoni/imunologia
Subpopulações de Linfócitos T/imunologia
Células Th1/imunologia
Triose-Fosfato Isomerase/imunologia
Animais
Citocinas/biossíntese
Células Dendríticas/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Humanos
Ativação Linfocitária/imunologia
Peptídeos/imunologia
title_short Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
title_full Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
title_fullStr Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
title_full_unstemmed Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
title_sort Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile.
author Reis, Eliana Almeida Gomes
author_facet Reis, Eliana Almeida Gomes
Carmo, Theomira Mauadie de Azevedo
Athanazio, Rodrigo
Reis, Mitermayer Galvão dos
Harn Junior, Donald A
author_role author
author2 Carmo, Theomira Mauadie de Azevedo
Athanazio, Rodrigo
Reis, Mitermayer Galvão dos
Harn Junior, Donald A
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Reis, Eliana Almeida Gomes
Carmo, Theomira Mauadie de Azevedo
Athanazio, Rodrigo
Reis, Mitermayer Galvão dos
Harn Junior, Donald A
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Helmintos/imunologia
Citocinas/imunologia
Schistosoma mansoni/imunologia
Subpopulações de Linfócitos T/imunologia
Células Th1/imunologia
Triose-Fosfato Isomerase/imunologia
Animais
Citocinas/biossíntese
Células Dendríticas/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Humanos
Ativação Linfocitária/imunologia
Peptídeos/imunologia
topic Antígenos de Helmintos/imunologia
Citocinas/imunologia
Schistosoma mansoni/imunologia
Subpopulações de Linfócitos T/imunologia
Células Th1/imunologia
Triose-Fosfato Isomerase/imunologia
Animais
Citocinas/biossíntese
Células Dendríticas/imunologia
Ensaio de Imunoadsorção Enzimática
Citometria de Fluxo
Humanos
Ativação Linfocitária/imunologia
Peptídeos/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2014-04-24T11:49:58Z
dc.date.available.fl_str_mv 2014-04-24T11:49:58Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv REIS, E. A. G. et al. Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile. Scandinavian Journal of Immunology, v. 68, n. 2, p. 169-176, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7530
dc.identifier.issn.none.fl_str_mv 1365-3083
dc.identifier.doi.none.fl_str_mv 10.1111/j.1365-3083.2008.02131.x
identifier_str_mv REIS, E. A. G. et al. Schistosoma mansoni triose phosphate isomerase peptide MAP4 is able to trigger naïve donor immune response towards a type-1 cytokine profile. Scandinavian Journal of Immunology, v. 68, n. 2, p. 169-176, 2008.
1365-3083
10.1111/j.1365-3083.2008.02131.x
url https://www.arca.fiocruz.br/handle/icict/7530
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Blackwell Publishing Ltd
publisher.none.fl_str_mv Blackwell Publishing Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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