Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação

Detalhes bibliográficos
Autor(a) principal: Zeraik, Ana Eliza
Data de Publicação: 2008
Outros Autores: Souza, Fernanda Sant'Ana de, Fatibello-Filho, Orlando, Leite, Oldair Donizeti
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/3884
Resumo: p. 731-734.
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spelling Zeraik, Ana ElizaSouza, Fernanda Sant'Ana deFatibello-Filho, OrlandoLeite, Oldair DonizetiZeraik, Ana ElizaSouza, Fernanda Sant'Ana deFatibello-Filho, OrlandoLeite, Oldair Donizeti2011-11-25T18:07:04Z2011-11-25T18:07:04Z20080100-4042http://www.repositorio.ufba.br/ri/handle/ri/3884v. 31, n. 4.p. 731-734.In this work we describe both a chromatographic purification procedure and a spot test for the enzyme peroxidase (POD: EC 1.11.1.7). The enzyme was obtained from crude extracts of sweet potatoes and the chromatographic enzyme purification procedure resulted in several fractions. Therefore a simple, fast and economic spot test for monitoring peroxidase during the purification procedure was developed. The spot test is based on the reaction of hydrogen peroxide and guaiacol, which is catalyzed by the presence of peroxidase yielding the colored tetraguaiacol.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2011-11-25T18:07:04Z No. of bitstreams: 1 a03v31n4.pdf: 153170 bytes, checksum: 9bd0d2486e8503ba59cffe32ec23ef63 (MD5)Made available in DSpace on 2011-11-25T18:07:04Z (GMT). No. of bitstreams: 1 a03v31n4.pdf: 153170 bytes, checksum: 9bd0d2486e8503ba59cffe32ec23ef63 (MD5) Previous issue date: 2008São PauloPurification procedurePeroxidaseSpot testDesenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificaçãoQuímica Novainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleporreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALa03v31n4.pdfa03v31n4.pdfapplication/pdf153170https://repositorio.ufba.br/bitstream/ri/3884/1/a03v31n4.pdf9bd0d2486e8503ba59cffe32ec23ef63MD51LICENSElicense.txtlicense.txttext/plain1906https://repositorio.ufba.br/bitstream/ri/3884/2/license.txtf312e2f45d1f6794aa5997ce60216324MD52TEXTa03v31n4.pdf.txta03v31n4.pdf.txtExtracted texttext/plain21536https://repositorio.ufba.br/bitstream/ri/3884/3/a03v31n4.pdf.txtd27c1ee47b22a77ac0bd39ca1189bd59MD53ri/38842022-07-05 14:03:18.973oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:18Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
dc.title.alternative.pt_BR.fl_str_mv Química Nova
title Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
spellingShingle Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
Zeraik, Ana Eliza
Purification procedure
Peroxidase
Spot test
title_short Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
title_full Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
title_fullStr Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
title_full_unstemmed Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
title_sort Desenvolvimento de um spot test para o monitoramento da atividade da peroxidase em um procedimento de purificação
author Zeraik, Ana Eliza
author_facet Zeraik, Ana Eliza
Souza, Fernanda Sant'Ana de
Fatibello-Filho, Orlando
Leite, Oldair Donizeti
author_role author
author2 Souza, Fernanda Sant'Ana de
Fatibello-Filho, Orlando
Leite, Oldair Donizeti
author2_role author
author
author
dc.contributor.author.fl_str_mv Zeraik, Ana Eliza
Souza, Fernanda Sant'Ana de
Fatibello-Filho, Orlando
Leite, Oldair Donizeti
Zeraik, Ana Eliza
Souza, Fernanda Sant'Ana de
Fatibello-Filho, Orlando
Leite, Oldair Donizeti
dc.subject.por.fl_str_mv Purification procedure
Peroxidase
Spot test
topic Purification procedure
Peroxidase
Spot test
description p. 731-734.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2011-11-25T18:07:04Z
dc.date.available.fl_str_mv 2011-11-25T18:07:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/3884
dc.identifier.issn.none.fl_str_mv 0100-4042
dc.identifier.number.pt_BR.fl_str_mv v. 31, n. 4.
identifier_str_mv 0100-4042
v. 31, n. 4.
url http://www.repositorio.ufba.br/ri/handle/ri/3884
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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