Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients

Detalhes bibliográficos
Autor(a) principal: Carvalho, Lain Carlos Pontes de
Data de Publicação: 2005
Outros Autores: Passos, S., Dutra, Walderez Ornelas, Soto, Manuel, Alonso, Carlos, Gollob, K. J., Carvalho Filho, Edgar Marcelino de, Jesus, A. Ribeiro de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6562
Resumo: Trabalho completo: acesso restrito, p. 337–342
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spelling Carvalho, Lain Carlos Pontes dePassos, S.Dutra, Walderez OrnelasSoto, ManuelAlonso, CarlosGollob, K. J.Carvalho Filho, Edgar Marcelino deJesus, A. Ribeiro deCarvalho, Lain Carlos Pontes dePassos, S.Dutra, Walderez OrnelasSoto, ManuelAlonso, CarlosGollob, K. J.Carvalho Filho, Edgar Marcelino deJesus, A. Ribeiro de2012-08-07T18:51:11Z2005-040300-9475http://www.repositorio.ufba.br/ri/handle/ri/6562v. 61, n. 4Trabalho completo: acesso restrito, p. 337–342The immune modulatory properties of recombinant antigens Kinetoplasmid membrane protein-11 (KMP11) and Leishmania homologue of receptors for activated C kinase (LACK) in cutaneous leishmaniasis (CL) and mucosal leishmaniasis (ML) patients were evaluated. The mean levels of interferon-γ (IFN-γ) in soluble leishmania antigen (SLA) stimulated peripheral blood mononuclear cells (PBMC) of ML and CL patients were 5625 ± 2333 pg/ml and 4422 ± 3665 pg/ml, respectively. IFN-γ was not detected in cultures stimulated with KMP11 or LACK. Interleukin-10 (IL-10) concentration in SLA, KMP11 and LACK-stimulated PBMC of ML patients was 13 ± 12 pg/ml, 285 ± 388 pg/ml and 802 ± 483 pg/ml, respectively. Addition of KMP11 or LACK to SLA-stimulated PBMC of CL and ML patients enhanced IL-10 production (P < 0.05). Addition of KMP11 decreased IFN-γ levels by 52% in CL patients and by 19% in ML patients. Addition of LACK to SLA-stimulated cultures decreased IFN-γ levels by 58% in CL patients and by 30% in ML patients. Neutralization of IL-10 abrogated the downregulatory effect of LACK and KMP11. The modulatory properties of LACK and KMP11 are due to induction of IL-10 production and may be helpful for attenuating chronic inflammatory diseases. However, in some clinical conditions, as demonstrated for ML, these molecules are not able to suppress the IFN-γ response, even inducing IL-10 production.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-08-07T18:51:11Z No. of bitstreams: 1 (153).pdf: 115873 bytes, checksum: 425c9ea05fcf40266c358853a1471e5b (MD5)Made available in DSpace on 2012-08-07T18:51:11Z (GMT). No. of bitstreams: 1 (153).pdf: 115873 bytes, checksum: 425c9ea05fcf40266c358853a1471e5b (MD5) Previous issue date: 2005-04Blackwell Publishinghttp://dx.doi.org/10.1111/j.1365-3083.2005.01581.xreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAEffect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis PatientsScandinavian Journal of Immunologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/6562/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINAL(153).pdf(153).pdfapplication/pdf115873https://repositorio.ufba.br/bitstream/ri/6562/1/%28153%29.pdf425c9ea05fcf40266c358853a1471e5bMD51TEXT(153).pdf.txt(153).pdf.txtExtracted texttext/plain29951https://repositorio.ufba.br/bitstream/ri/6562/3/%28153%29.pdf.txtee25b2316b08d402ac0a7d05e6cb7f60MD53ri/65622022-07-05 14:03:10.579oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:10Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
dc.title.alternative.pt_BR.fl_str_mv Scandinavian Journal of Immunology
title Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
spellingShingle Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
Carvalho, Lain Carlos Pontes de
title_short Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
title_full Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
title_fullStr Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
title_full_unstemmed Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
title_sort Effect of LACK and KMP11 on IFN-γ Production by Peripheral Blood Mononuclear Cells from Cutaneous and Mucosal Leishmaniasis Patients
author Carvalho, Lain Carlos Pontes de
author_facet Carvalho, Lain Carlos Pontes de
Passos, S.
Dutra, Walderez Ornelas
Soto, Manuel
Alonso, Carlos
Gollob, K. J.
Carvalho Filho, Edgar Marcelino de
Jesus, A. Ribeiro de
author_role author
author2 Passos, S.
Dutra, Walderez Ornelas
Soto, Manuel
Alonso, Carlos
Gollob, K. J.
Carvalho Filho, Edgar Marcelino de
Jesus, A. Ribeiro de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Lain Carlos Pontes de
Passos, S.
Dutra, Walderez Ornelas
Soto, Manuel
Alonso, Carlos
Gollob, K. J.
Carvalho Filho, Edgar Marcelino de
Jesus, A. Ribeiro de
Carvalho, Lain Carlos Pontes de
Passos, S.
Dutra, Walderez Ornelas
Soto, Manuel
Alonso, Carlos
Gollob, K. J.
Carvalho Filho, Edgar Marcelino de
Jesus, A. Ribeiro de
description Trabalho completo: acesso restrito, p. 337–342
publishDate 2005
dc.date.issued.fl_str_mv 2005-04
dc.date.accessioned.fl_str_mv 2012-08-07T18:51:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6562
dc.identifier.issn.none.fl_str_mv 0300-9475
dc.identifier.number.pt_BR.fl_str_mv v. 61, n. 4
identifier_str_mv 0300-9475
v. 61, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/6562
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1111/j.1365-3083.2005.01581.x
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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