Effects of perinatal asphyxia on rat striatal cytoskeleton

Detalhes bibliográficos
Autor(a) principal: Saraceno, Gustavo Ezequiel
Data de Publicação: 2012
Outros Autores: Ayala, M. V., Badorrey, Maria Sol, Holubiec, M., Romero, J. I., Galeano, P., Barreto, George Emílio Sampaio, Giraldez Alvárez, L. D., Fres, R. Kölliker, Coirini, H., Capani, Francisco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16505
Resumo: Texto completo: acesso restrito. p. 9-19
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spelling Saraceno, Gustavo EzequielAyala, M. V.Badorrey, Maria SolHolubiec, M.Romero, J. I.Galeano, P.Barreto, George Emílio SampaioGiraldez Alvárez, L. D.Fres, R. KöllikerCoirini, H.Capani, FranciscoSaraceno, Gustavo EzequielAyala, M. V.Badorrey, Maria SolHolubiec, M.Romero, J. I.Galeano, P.Barreto, George Emílio SampaioGiraldez Alvárez, L. D.Fres, R. KöllikerCoirini, H.Capani, Francisco2014-11-04T15:08:33Z20120887-4476http://repositorio.ufba.br/ri/handle/ri/16505v. 66, n. 1Texto completo: acesso restrito. p. 9-19Perinatal asphyxia (PA) is a medical condition associated with a high short-term morbimortality and different long-term neurological diseases. In previous works, we have shown that neuronal and synaptic changes in rat striatum lead to ubi-protein accumulation in post-synaptic density (PSD) after six months of sub-severe PA. However, very little is known about the synaptic and related structural modifications induced by PA in young rats. In the present work, we studied neuronal cytoskeleton modifications in striatum induced by subsevere PA in 30-day-old rats. We observed a significant decrease in the number of neurons, in particular calbindin immunoreactive neurons after PA. In addition, it was also observed that actin cytoskeleton was highly modified in the PSD as well as an increment of F-actin staining by Phalloidin-alexa488 in the striatum of PA rats. Using correlative fluorescence-electron microscopy photooxidation, we confirmed and extended confocal observations. F-actin staining augmentation was mostly related with an increment in the number of mushroom-shaped spines. Consistent with microscopic data, Western blot analysis revealed a β-actin increment in PSD in PA rats. On the other hand, MAP-2 immunostaining was decreased after PA, being NF-200 expression unmodified. Although neuronal death was observed, signs of generalized neurodegeneration were absent. Taken together these results showed early post-synaptic F-actin cytoskeleton changes induced by PA with slightly modifications in the other components of the neuronal cytoskeleton, suggesting that F-actin accumulation in the dendritic spines could be involved in the neuronal loss induced by PA.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-11-04T11:40:58Z No. of bitstreams: 1 G. E. Saraceno.pdf: 3247047 bytes, checksum: fee9d67d82352471a93e9554ef1f1be6 (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-11-04T15:08:33Z (GMT) No. of bitstreams: 1 G. E. Saraceno.pdf: 3247047 bytes, checksum: fee9d67d82352471a93e9554ef1f1be6 (MD5)Made available in DSpace on 2014-11-04T15:08:33Z (GMT). No. of bitstreams: 1 G. E. Saraceno.pdf: 3247047 bytes, checksum: fee9d67d82352471a93e9554ef1f1be6 (MD5) Previous issue date: 2012http://dx.doi.org/10.1002/syn.20978reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAStriatumPerinatal asphyxiaMap-2Neuronal cytoskeletonDendritic spinesEffects of perinatal asphyxia on rat striatal cytoskeletonSynapseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALG. E. Saraceno.pdfG. E. Saraceno.pdfapplication/pdf3247047https://repositorio.ufba.br/bitstream/ri/16505/1/G.%20E.%20Saraceno.pdffee9d67d82352471a93e9554ef1f1be6MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16505/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTG. E. Saraceno.pdf.txtG. E. 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dc.title.pt_BR.fl_str_mv Effects of perinatal asphyxia on rat striatal cytoskeleton
dc.title.alternative.pt_BR.fl_str_mv Synapse
title Effects of perinatal asphyxia on rat striatal cytoskeleton
spellingShingle Effects of perinatal asphyxia on rat striatal cytoskeleton
Saraceno, Gustavo Ezequiel
Striatum
Perinatal asphyxia
Map-2
Neuronal cytoskeleton
Dendritic spines
title_short Effects of perinatal asphyxia on rat striatal cytoskeleton
title_full Effects of perinatal asphyxia on rat striatal cytoskeleton
title_fullStr Effects of perinatal asphyxia on rat striatal cytoskeleton
title_full_unstemmed Effects of perinatal asphyxia on rat striatal cytoskeleton
title_sort Effects of perinatal asphyxia on rat striatal cytoskeleton
author Saraceno, Gustavo Ezequiel
author_facet Saraceno, Gustavo Ezequiel
Ayala, M. V.
Badorrey, Maria Sol
Holubiec, M.
Romero, J. I.
Galeano, P.
Barreto, George Emílio Sampaio
Giraldez Alvárez, L. D.
Fres, R. Kölliker
Coirini, H.
Capani, Francisco
author_role author
author2 Ayala, M. V.
Badorrey, Maria Sol
Holubiec, M.
Romero, J. I.
Galeano, P.
Barreto, George Emílio Sampaio
Giraldez Alvárez, L. D.
Fres, R. Kölliker
Coirini, H.
Capani, Francisco
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Saraceno, Gustavo Ezequiel
Ayala, M. V.
Badorrey, Maria Sol
Holubiec, M.
Romero, J. I.
Galeano, P.
Barreto, George Emílio Sampaio
Giraldez Alvárez, L. D.
Fres, R. Kölliker
Coirini, H.
Capani, Francisco
Saraceno, Gustavo Ezequiel
Ayala, M. V.
Badorrey, Maria Sol
Holubiec, M.
Romero, J. I.
Galeano, P.
Barreto, George Emílio Sampaio
Giraldez Alvárez, L. D.
Fres, R. Kölliker
Coirini, H.
Capani, Francisco
dc.subject.por.fl_str_mv Striatum
Perinatal asphyxia
Map-2
Neuronal cytoskeleton
Dendritic spines
topic Striatum
Perinatal asphyxia
Map-2
Neuronal cytoskeleton
Dendritic spines
description Texto completo: acesso restrito. p. 9-19
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-11-04T15:08:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16505
dc.identifier.issn.none.fl_str_mv 0887-4476
dc.identifier.number.pt_BR.fl_str_mv v. 66, n. 1
identifier_str_mv 0887-4476
v. 66, n. 1
url http://repositorio.ufba.br/ri/handle/ri/16505
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1002/syn.20978
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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collection Repositório Institucional da UFBA
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