Monitoring Leptospira strain collections: the need for quality control

Detalhes bibliográficos
Autor(a) principal: Cerqueira, Gustavo M.
Data de Publicação: 2010
Outros Autores: McBride, Alan J. A., Queiroz, Adriano, Pinto, Luciano S., Silva, Éverton F., Hartskeerl, Rudy A., Reis, Mitermayer G., Ko, Albert I., Dellagostin, Odir Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/12938
Resumo: Texto completo: acesso restrito. p.83-87
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spelling Cerqueira, Gustavo M.McBride, Alan J. A.Queiroz, AdrianoPinto, Luciano S.Silva, Éverton F.Hartskeerl, Rudy A.Reis, Mitermayer G.Ko, Albert I.Dellagostin, Odir AntonioCerqueira, Gustavo M.McBride, Alan J. A.Queiroz, AdrianoPinto, Luciano S.Silva, Éverton F.Hartskeerl, Rudy A.Reis, Mitermayer G.Ko, Albert I.Dellagostin, Odir Antonio2013-09-13T17:18:48Z20100002-9637http://www.repositorio.ufba.br/ri/handle/ri/12938v. 82, n. 1Texto completo: acesso restrito. p.83-87The purpose of this study was to perform a 16S sequence-based quality control of two Leptospira strain collections. 16S rRNA gene sequencing was used to verify two Leptospira reference collections provided by the World Health Organization and maintained at a reference laboratory for leptospirosis in Brazil. Among the 89 serovars evaluated, four conflicting strains were identified in one of the collections. Although 16S rRNA gene sequencing cannot identify Leptospira beyond the species level, it is suitable for the identification of contamination and quality control of leptospiral reference collections. This study highlights the importance of the availability of high-quality 16S rRNA sequences in public databases. In addition, it emphasizes the need for periodical verifications and quality control of Leptospira reference collections.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-09-11T13:14:58Z No. of bitstreams: 1 2010.09-0558.pdf: 485495 bytes, checksum: b03bac0e10bd69c6989e1ca198a5b1c1 (MD5)Approved for entry into archive by Vilma Conceição(vilmagc@ufba.br) on 2013-09-13T17:18:48Z (GMT) No. of bitstreams: 1 2010.09-0558.pdf: 485495 bytes, checksum: b03bac0e10bd69c6989e1ca198a5b1c1 (MD5)Made available in DSpace on 2013-09-13T17:18:48Z (GMT). No. of bitstreams: 1 2010.09-0558.pdf: 485495 bytes, checksum: b03bac0e10bd69c6989e1ca198a5b1c1 (MD5) Previous issue date: 2010http://dx.doi.org/10.4269/ajtmh.2010.09-0558reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALeptospiraLeptospiroseContaminaçãoLeptospirosisContaminationMonitoring Leptospira strain collections: the need for quality controlAmerican Journal of Tropical Medicine and Hygieneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12938/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52ORIGINAL2010.09-0558.pdf2010.09-0558.pdfapplication/pdf485495https://repositorio.ufba.br/bitstream/ri/12938/1/2010.09-0558.pdfb03bac0e10bd69c6989e1ca198a5b1c1MD51TEXT2010.09-0558.pdf.txt2010.09-0558.pdf.txtExtracted texttext/plain27684https://repositorio.ufba.br/bitstream/ri/12938/3/2010.09-0558.pdf.txt398853174631914d31dc638b87117df5MD53ri/129382022-07-05 14:03:44.279oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:44Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Monitoring Leptospira strain collections: the need for quality control
dc.title.alternative.pt_BR.fl_str_mv American Journal of Tropical Medicine and Hygiene
title Monitoring Leptospira strain collections: the need for quality control
spellingShingle Monitoring Leptospira strain collections: the need for quality control
Cerqueira, Gustavo M.
Leptospira
Leptospirose
Contaminação
Leptospirosis
Contamination
title_short Monitoring Leptospira strain collections: the need for quality control
title_full Monitoring Leptospira strain collections: the need for quality control
title_fullStr Monitoring Leptospira strain collections: the need for quality control
title_full_unstemmed Monitoring Leptospira strain collections: the need for quality control
title_sort Monitoring Leptospira strain collections: the need for quality control
author Cerqueira, Gustavo M.
author_facet Cerqueira, Gustavo M.
McBride, Alan J. A.
Queiroz, Adriano
Pinto, Luciano S.
Silva, Éverton F.
Hartskeerl, Rudy A.
Reis, Mitermayer G.
Ko, Albert I.
Dellagostin, Odir Antonio
author_role author
author2 McBride, Alan J. A.
Queiroz, Adriano
Pinto, Luciano S.
Silva, Éverton F.
Hartskeerl, Rudy A.
Reis, Mitermayer G.
Ko, Albert I.
Dellagostin, Odir Antonio
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cerqueira, Gustavo M.
McBride, Alan J. A.
Queiroz, Adriano
Pinto, Luciano S.
Silva, Éverton F.
Hartskeerl, Rudy A.
Reis, Mitermayer G.
Ko, Albert I.
Dellagostin, Odir Antonio
Cerqueira, Gustavo M.
McBride, Alan J. A.
Queiroz, Adriano
Pinto, Luciano S.
Silva, Éverton F.
Hartskeerl, Rudy A.
Reis, Mitermayer G.
Ko, Albert I.
Dellagostin, Odir Antonio
dc.subject.por.fl_str_mv Leptospira
Leptospirose
Contaminação
Leptospirosis
Contamination
topic Leptospira
Leptospirose
Contaminação
Leptospirosis
Contamination
description Texto completo: acesso restrito. p.83-87
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-09-13T17:18:48Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/12938
dc.identifier.issn.none.fl_str_mv 0002-9637
dc.identifier.number.pt_BR.fl_str_mv v. 82, n. 1
identifier_str_mv 0002-9637
v. 82, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/12938
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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