Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis

Detalhes bibliográficos
Autor(a) principal: Keyburn, Anthony L.
Data de Publicação: 2013
Outros Autores: Portela, Ricardo Wagner Dias, Sproat, Kathy, Ford, Mark E., Bannam, Trudi L., Xuxia, Yan, Rood, Julian I., Moore, Robert J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/15218
Resumo: p. 1-8
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spelling Keyburn, Anthony L.Portela, Ricardo Wagner DiasSproat, KathyFord, Mark E.Bannam, Trudi L.Xuxia, YanRood, Julian I.Moore, Robert J.Keyburn, Anthony L.Portela, Ricardo Wagner DiasSproat, KathyFord, Mark E.Bannam, Trudi L.Xuxia, YanRood, Julian I.Moore, Robert J.2014-07-16T16:27:57Z2014-07-16T16:27:57Z20130928-4249http://repositorio.ufba.br/ri/handle/ri/15218v. 44, n. 1p. 1-8NetB toxin from Clostridium perfringens is a major virulence factor in necrotic enteritis in poultry. In this study the efficacy of NetB as a vaccine antigen to protect chickens from necrotic enteritis was examined. Broiler chickens were immunized subcutaneously with purified recombinant NetB (rNetB), formalin treated bacterin and cell free toxoid with or without rNetB supplementation. Intestinal lesion scores and NetB antibody levels were measured to determine protection after mild oral gavage, moderate in-feed and heavy in-feed challenges with virulent C. perfringens isolates. Birds immunized with rNetB were significantly protected against necrotic enteritis when challenged with a mild oral dose of virulent bacteria, but were not protected when a more robust challenge was used. Bacterin and cell free toxoid without rNetB supplementation did not protect birds from moderate and severe in-feed challenge. Only birds immunized with bacterin and cell free toxoid supplemented with rNetB showed significant protection against moderate and severe in-feed challenge, with the later giving the greatest protection. Higher NetB antibody titres were observed in birds immunized with rNetB compared to those vaccinated with bacterin or toxoid, suggesting that the in vitro levels of NetB produced by virulent C. perfringens isolates are too low to induce the development of a strong immune response. These results suggest that vaccination with NetB alone may not be sufficient to protect birds from necrotic enteritis in the field, but that in combination with other cellular or cell-free antigens it can significantly protect chickens from disease.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-05-15T13:48:07Z No. of bitstreams: 1 1297-9716-44-54.pdf: 554821 bytes, checksum: 53ecde51771fcb4438e4654bdc918137 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-07-16T16:27:57Z (GMT) No. of bitstreams: 1 1297-9716-44-54.pdf: 554821 bytes, checksum: 53ecde51771fcb4438e4654bdc918137 (MD5)Made available in DSpace on 2014-07-16T16:27:57Z (GMT). No. of bitstreams: 1 1297-9716-44-54.pdf: 554821 bytes, checksum: 53ecde51771fcb4438e4654bdc918137 (MD5) Previous issue date: 2013http://dx.doi.org/10.1186/1297-9716-44-54reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAVaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritisVeterinary Researchinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINAL1297-9716-44-54.pdf1297-9716-44-54.pdfapplication/pdf554821https://repositorio.ufba.br/bitstream/ri/15218/1/1297-9716-44-54.pdf53ecde51771fcb4438e4654bdc918137MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/15218/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT1297-9716-44-54.pdf.txt1297-9716-44-54.pdf.txtExtracted texttext/plain38883https://repositorio.ufba.br/bitstream/ri/15218/3/1297-9716-44-54.pdf.txtd251894df06e3beaf5fe4914643989daMD53ri/152182022-08-08 13:18:19.448oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:18:19Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
dc.title.alternative.pt_BR.fl_str_mv Veterinary Research
title Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
spellingShingle Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
Keyburn, Anthony L.
title_short Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
title_full Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
title_fullStr Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
title_full_unstemmed Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
title_sort Vaccination with recombinant NetB toxin partially protects broiler chickens from necrotic enteritis
author Keyburn, Anthony L.
author_facet Keyburn, Anthony L.
Portela, Ricardo Wagner Dias
Sproat, Kathy
Ford, Mark E.
Bannam, Trudi L.
Xuxia, Yan
Rood, Julian I.
Moore, Robert J.
author_role author
author2 Portela, Ricardo Wagner Dias
Sproat, Kathy
Ford, Mark E.
Bannam, Trudi L.
Xuxia, Yan
Rood, Julian I.
Moore, Robert J.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Keyburn, Anthony L.
Portela, Ricardo Wagner Dias
Sproat, Kathy
Ford, Mark E.
Bannam, Trudi L.
Xuxia, Yan
Rood, Julian I.
Moore, Robert J.
Keyburn, Anthony L.
Portela, Ricardo Wagner Dias
Sproat, Kathy
Ford, Mark E.
Bannam, Trudi L.
Xuxia, Yan
Rood, Julian I.
Moore, Robert J.
description p. 1-8
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-07-16T16:27:57Z
dc.date.available.fl_str_mv 2014-07-16T16:27:57Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/15218
dc.identifier.issn.none.fl_str_mv 0928-4249
dc.identifier.number.pt_BR.fl_str_mv v. 44, n. 1
identifier_str_mv 0928-4249
v. 44, n. 1
url http://repositorio.ufba.br/ri/handle/ri/15218
dc.language.iso.fl_str_mv eng
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